Ginkgo biloba extract (EGb761) inhibits autophagy and apoptosis in a rat model of vascular dementia via the AMPK-mTOR signalling pathway
Vascular dementia (VD), a chronic syndrome of acquired intellectual impairment resulting from cerebrovascular diseases, is closely linked to neuronal autophagy and apoptosis. As a standardized extract from Ginkgo biloba L. leaves, EGb761 is widely applied for treating cerebrovascular diseases owing...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624001701 |
| _version_ | 1797215934064099328 |
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| author | Yue Yin Chunyan Yan Rui Zhang Yuliang Wang Yang Song Shujuan Hu Xin Zhao Ruofan Liu Minghao Guo Yaxuan Wang Xin Cai Dexiu Wang |
| author_facet | Yue Yin Chunyan Yan Rui Zhang Yuliang Wang Yang Song Shujuan Hu Xin Zhao Ruofan Liu Minghao Guo Yaxuan Wang Xin Cai Dexiu Wang |
| author_sort | Yue Yin |
| collection | DOAJ |
| description | Vascular dementia (VD), a chronic syndrome of acquired intellectual impairment resulting from cerebrovascular diseases, is closely linked to neuronal autophagy and apoptosis. As a standardized extract from Ginkgo biloba L. leaves, EGb761 is widely applied for treating cerebrovascular diseases owing to its neuroprotective effects, with on-going research exploring its therapeutic mechanisms. Rat model of VD and SH-SY5Y cell model with OGD/R injury was applied for this study. Results showed that EGb761 reduces OGD/R-elicited apoptosis and autophagosome production, whereas improving the viability of hippocampal neurons. EGb761 treatment led to a decrease in the ratios of p-AMPK/AMPK, Bax/Bcl-2, cleaved caspase-3/caspase-3, cleaved caspase-9/caspase-9, LC3-II/LC3-I, and level of Beciln-1, as well as enhancing the proportion of p-mTOR/mTOR. Additionally, EGb761 indicated a protective effect on rats' cognitive function. These results demonstrated EGb761′s specific therapeutic mechanism in preventing apoptosis and autophagy in VD models and improving cognitive functioning in VD rats through AMPK-mTOR signalling. |
| first_indexed | 2024-04-24T11:37:57Z |
| format | Article |
| id | doaj.art-057420e74c4a45d38e7792df68f34fea |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-24T11:37:57Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-057420e74c4a45d38e7792df68f34fea2024-04-10T04:28:51ZengElsevierJournal of Functional Foods1756-46462024-05-01116106168Ginkgo biloba extract (EGb761) inhibits autophagy and apoptosis in a rat model of vascular dementia via the AMPK-mTOR signalling pathwayYue Yin0Chunyan Yan1Rui Zhang2Yuliang Wang3Yang Song4Shujuan Hu5Xin Zhao6Ruofan Liu7Minghao Guo8Yaxuan Wang9Xin Cai10Dexiu Wang11School of Basic Medical Sciences, Shandong Second Medical University, Weifang 261053, ChinaSchool of Basic Medical Sciences, Shandong Second Medical University, Weifang 261053, ChinaEncephalopathy Intensive Emergency Department, Weifang Traditional Chinese Hospital, Weifang 261000, ChinaSchool of Basic Medical Sciences, Shandong Second Medical University, Weifang 261053, ChinaSchool of Clinical Medicine, Shandong Second Medical University, Weifang 261053, ChinaSchool of Basic Medical Sciences, Shandong Second Medical University, Weifang 261053, ChinaGeriatric Medicine Department, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, ChinaGeriatric Medicine Department, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, ChinaGeriatric Medicine Department, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, ChinaSchool of Clinical Medicine, Shandong Second Medical University, Weifang 261053, ChinaSchool of Basic Medical Sciences, Shandong Second Medical University, Weifang 261053, China; Corresponding authors at: Shandong Second Medical University, No.7166 Baotong West Street, Weifang 261053, China.School of Basic Medical Sciences, Shandong Second Medical University, Weifang 261053, China; Corresponding authors at: Shandong Second Medical University, No.7166 Baotong West Street, Weifang 261053, China.Vascular dementia (VD), a chronic syndrome of acquired intellectual impairment resulting from cerebrovascular diseases, is closely linked to neuronal autophagy and apoptosis. As a standardized extract from Ginkgo biloba L. leaves, EGb761 is widely applied for treating cerebrovascular diseases owing to its neuroprotective effects, with on-going research exploring its therapeutic mechanisms. Rat model of VD and SH-SY5Y cell model with OGD/R injury was applied for this study. Results showed that EGb761 reduces OGD/R-elicited apoptosis and autophagosome production, whereas improving the viability of hippocampal neurons. EGb761 treatment led to a decrease in the ratios of p-AMPK/AMPK, Bax/Bcl-2, cleaved caspase-3/caspase-3, cleaved caspase-9/caspase-9, LC3-II/LC3-I, and level of Beciln-1, as well as enhancing the proportion of p-mTOR/mTOR. Additionally, EGb761 indicated a protective effect on rats' cognitive function. These results demonstrated EGb761′s specific therapeutic mechanism in preventing apoptosis and autophagy in VD models and improving cognitive functioning in VD rats through AMPK-mTOR signalling.http://www.sciencedirect.com/science/article/pii/S1756464624001701Ginkgo biloba extract (EGb761)Vascular dementiaAutophagyApoptosisAMPK-mTOR pathway |
| spellingShingle | Yue Yin Chunyan Yan Rui Zhang Yuliang Wang Yang Song Shujuan Hu Xin Zhao Ruofan Liu Minghao Guo Yaxuan Wang Xin Cai Dexiu Wang Ginkgo biloba extract (EGb761) inhibits autophagy and apoptosis in a rat model of vascular dementia via the AMPK-mTOR signalling pathway Journal of Functional Foods Ginkgo biloba extract (EGb761) Vascular dementia Autophagy Apoptosis AMPK-mTOR pathway |
| title | Ginkgo biloba extract (EGb761) inhibits autophagy and apoptosis in a rat model of vascular dementia via the AMPK-mTOR signalling pathway |
| title_full | Ginkgo biloba extract (EGb761) inhibits autophagy and apoptosis in a rat model of vascular dementia via the AMPK-mTOR signalling pathway |
| title_fullStr | Ginkgo biloba extract (EGb761) inhibits autophagy and apoptosis in a rat model of vascular dementia via the AMPK-mTOR signalling pathway |
| title_full_unstemmed | Ginkgo biloba extract (EGb761) inhibits autophagy and apoptosis in a rat model of vascular dementia via the AMPK-mTOR signalling pathway |
| title_short | Ginkgo biloba extract (EGb761) inhibits autophagy and apoptosis in a rat model of vascular dementia via the AMPK-mTOR signalling pathway |
| title_sort | ginkgo biloba extract egb761 inhibits autophagy and apoptosis in a rat model of vascular dementia via the ampk mtor signalling pathway |
| topic | Ginkgo biloba extract (EGb761) Vascular dementia Autophagy Apoptosis AMPK-mTOR pathway |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624001701 |
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