Dissecting the role of CSF2RB expression in human regulatory T cells
Colony stimulating factor 2 receptor subunit beta (CSF2RB; CD131) is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. Interestingly, FOXP3+ regulatory T cells (Tregs), which play a pivotal role in preventi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1005965/full |
| _version_ | 1828097698665857024 |
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| author | Beatriz F. Côrte-Real Beatriz F. Côrte-Real Rebeca Arroyo Hornero Rebeca Arroyo Hornero Aleksandra Dyczko Aleksandra Dyczko Ibrahim Hamad Ibrahim Hamad Markus Kleinewietfeld Markus Kleinewietfeld Markus Kleinewietfeld |
| author_facet | Beatriz F. Côrte-Real Beatriz F. Côrte-Real Rebeca Arroyo Hornero Rebeca Arroyo Hornero Aleksandra Dyczko Aleksandra Dyczko Ibrahim Hamad Ibrahim Hamad Markus Kleinewietfeld Markus Kleinewietfeld Markus Kleinewietfeld |
| author_sort | Beatriz F. Côrte-Real |
| collection | DOAJ |
| description | Colony stimulating factor 2 receptor subunit beta (CSF2RB; CD131) is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. Interestingly, FOXP3+ regulatory T cells (Tregs), which play a pivotal role in prevention of autoimmunity have been demonstrated to highly overexpress CSF2RB and genome-wide association studies (GWAS) identified CSF2RB as being linked to autoimmune diseases like multiple sclerosis (MS). However, the exact biological role of CD131 in human Tregs has not been defined yet. Here we investigated CD131 importance on Treg phenotype and function in a broad range of in vitro studies. Although we could not recognize a specific function of CSF2RB; CD131 in human Tregs, our data show that CD131 expression is vastly restricted to Tregs even under stimulatory conditions, indicating that CD131 could aid as a potential marker to identify Treg subpopulations from pools of activated CD4+ T cells. Importantly, our analysis further demonstrate the overexpression of CSF2RB in Tregs of patients with autoimmune diseases like MS and systemic lupus erythematosus (SLE) in comparison to healthy controls, thereby indicating that CSF2RB expression in Tregs could serve as a potential novel biomarker for disease. |
| first_indexed | 2024-04-11T07:50:05Z |
| format | Article |
| id | doaj.art-057b25e05551434a8810ffc4712b8def |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T07:50:05Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-057b25e05551434a8810ffc4712b8def2022-12-22T04:36:06ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-12-011310.3389/fimmu.2022.10059651005965Dissecting the role of CSF2RB expression in human regulatory T cellsBeatriz F. Côrte-Real0Beatriz F. Côrte-Real1Rebeca Arroyo Hornero2Rebeca Arroyo Hornero3Aleksandra Dyczko4Aleksandra Dyczko5Ibrahim Hamad6Ibrahim Hamad7Markus Kleinewietfeld8Markus Kleinewietfeld9Markus Kleinewietfeld10Vlaams Instituut voor Biotechnologie (VIB) Laboratory of Translational Immunomodulation, Vlaams Instituut voor Biotechnologie (VIB) Center for Inflammation Research (IRC), Hasselt University, Diepenbeek, BelgiumDepartment of Immunology, Biomedical Research Institute, Hasselt University, Diepenbeek, BelgiumVlaams Instituut voor Biotechnologie (VIB) Laboratory of Translational Immunomodulation, Vlaams Instituut voor Biotechnologie (VIB) Center for Inflammation Research (IRC), Hasselt University, Diepenbeek, BelgiumDepartment of Immunology, Biomedical Research Institute, Hasselt University, Diepenbeek, BelgiumVlaams Instituut voor Biotechnologie (VIB) Laboratory of Translational Immunomodulation, Vlaams Instituut voor Biotechnologie (VIB) Center for Inflammation Research (IRC), Hasselt University, Diepenbeek, BelgiumDepartment of Immunology, Biomedical Research Institute, Hasselt University, Diepenbeek, BelgiumVlaams Instituut voor Biotechnologie (VIB) Laboratory of Translational Immunomodulation, Vlaams Instituut voor Biotechnologie (VIB) Center for Inflammation Research (IRC), Hasselt University, Diepenbeek, BelgiumDepartment of Immunology, Biomedical Research Institute, Hasselt University, Diepenbeek, BelgiumVlaams Instituut voor Biotechnologie (VIB) Laboratory of Translational Immunomodulation, Vlaams Instituut voor Biotechnologie (VIB) Center for Inflammation Research (IRC), Hasselt University, Diepenbeek, BelgiumDepartment of Immunology, Biomedical Research Institute, Hasselt University, Diepenbeek, BelgiumUniversity Mulpitle Sclerosis Center (UMSC), Hasselt University (UHasselt)/Campus, Diepenbeek, BelgiumColony stimulating factor 2 receptor subunit beta (CSF2RB; CD131) is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. Interestingly, FOXP3+ regulatory T cells (Tregs), which play a pivotal role in prevention of autoimmunity have been demonstrated to highly overexpress CSF2RB and genome-wide association studies (GWAS) identified CSF2RB as being linked to autoimmune diseases like multiple sclerosis (MS). However, the exact biological role of CD131 in human Tregs has not been defined yet. Here we investigated CD131 importance on Treg phenotype and function in a broad range of in vitro studies. Although we could not recognize a specific function of CSF2RB; CD131 in human Tregs, our data show that CD131 expression is vastly restricted to Tregs even under stimulatory conditions, indicating that CD131 could aid as a potential marker to identify Treg subpopulations from pools of activated CD4+ T cells. Importantly, our analysis further demonstrate the overexpression of CSF2RB in Tregs of patients with autoimmune diseases like MS and systemic lupus erythematosus (SLE) in comparison to healthy controls, thereby indicating that CSF2RB expression in Tregs could serve as a potential novel biomarker for disease.https://www.frontiersin.org/articles/10.3389/fimmu.2022.1005965/fullCSF2RBCD4 + T cellsFOXP3 + TregsautoimmunityMS |
| spellingShingle | Beatriz F. Côrte-Real Beatriz F. Côrte-Real Rebeca Arroyo Hornero Rebeca Arroyo Hornero Aleksandra Dyczko Aleksandra Dyczko Ibrahim Hamad Ibrahim Hamad Markus Kleinewietfeld Markus Kleinewietfeld Markus Kleinewietfeld Dissecting the role of CSF2RB expression in human regulatory T cells Frontiers in Immunology CSF2RB CD4 + T cells FOXP3 + Tregs autoimmunity MS |
| title | Dissecting the role of CSF2RB expression in human regulatory T cells |
| title_full | Dissecting the role of CSF2RB expression in human regulatory T cells |
| title_fullStr | Dissecting the role of CSF2RB expression in human regulatory T cells |
| title_full_unstemmed | Dissecting the role of CSF2RB expression in human regulatory T cells |
| title_short | Dissecting the role of CSF2RB expression in human regulatory T cells |
| title_sort | dissecting the role of csf2rb expression in human regulatory t cells |
| topic | CSF2RB CD4 + T cells FOXP3 + Tregs autoimmunity MS |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1005965/full |
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