Proteomics-based vaccine targets annotation and design of multi-epitope vaccine against antibiotic-resistant Streptococcus gallolyticus
Abstract Streptococcus gallolyticus is a non-motile, gram-positive bacterium that causes infective endocarditis. S. gallolyticus has developed resistance to existing antibiotics, and no vaccine is currently available. Therefore, it is essential to develop an effective S. gallolyticus vaccine. Core p...
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-55372-3 |
| _version_ | 1797274552488689664 |
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| author | Peng Chao Xueqin Zhang Lei Zhang Aiping Yang Yong Wang Xiaoyang Chen |
| author_facet | Peng Chao Xueqin Zhang Lei Zhang Aiping Yang Yong Wang Xiaoyang Chen |
| author_sort | Peng Chao |
| collection | DOAJ |
| description | Abstract Streptococcus gallolyticus is a non-motile, gram-positive bacterium that causes infective endocarditis. S. gallolyticus has developed resistance to existing antibiotics, and no vaccine is currently available. Therefore, it is essential to develop an effective S. gallolyticus vaccine. Core proteomics was used in this study together with subtractive proteomics and reverse vaccinology approach to find antigenic proteins that could be utilized for the design of the S. gallolyticus multi-epitope vaccine. The pipeline identified two antigenic proteins as potential vaccine targets: penicillin-binding protein and the ATP synthase subunit. T and B cell epitopes from the specific proteins were forecasted employing several immunoinformatics and bioinformatics resources. A vaccine (360 amino acids) was created using a combination of seven cytotoxic T cell lymphocyte (CTL), three helper T cell lymphocyte (HTL), and five linear B cell lymphocyte (LBL) epitopes. To increase immune responses, the vaccine was paired with a cholera enterotoxin subunit B (CTB) adjuvant. The developed vaccine was highly antigenic, non-allergenic, and stable for human use. The vaccine's binding affinity and molecular interactions with the human immunological receptor TLR4 were studied using molecular mechanics/generalized Born surface area (MMGBSA), molecular docking, and molecular dynamic (MD) simulation analyses. Escherichia coli (strain K12) plasmid vector pET-28a ( +) was used to examine the ability of the vaccine to be expressed. According to the outcomes of these computer experiments, the vaccine is quite promising in terms of developing a protective immunity against diseases. However, in vitro and animal research are required to validate our findings. |
| first_indexed | 2024-03-07T15:00:01Z |
| format | Article |
| id | doaj.art-057b73d582f546029a1a1945c0238a44 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:00:01Z |
| publishDate | 2024-02-01 |
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| series | Scientific Reports |
| spelling | doaj.art-057b73d582f546029a1a1945c0238a442024-03-05T19:11:02ZengNature PortfolioScientific Reports2045-23222024-02-0114111510.1038/s41598-024-55372-3Proteomics-based vaccine targets annotation and design of multi-epitope vaccine against antibiotic-resistant Streptococcus gallolyticusPeng Chao0Xueqin Zhang1Lei Zhang2Aiping Yang3Yong Wang4Xiaoyang Chen5Department of Cardiology, People’s Hospital of Xinjiang Uygur Autonomous RegionDepartment of Nephrology, People’s Hospital of Xinjiang Uygur Autonomous RegionDepartment of Cardiology, People’s Hospital of Xinjiang Uygur Autonomous RegionDepartment of Traditional Chinese Medicine, People’s Hospital of Xinjiang Uygur Autonomous RegionDepartment of Cardiology, People’s Hospital of Xinjiang Uygur Autonomous RegionDepartment of Cardiology, People’s Hospital of Xinjiang Uygur Autonomous RegionAbstract Streptococcus gallolyticus is a non-motile, gram-positive bacterium that causes infective endocarditis. S. gallolyticus has developed resistance to existing antibiotics, and no vaccine is currently available. Therefore, it is essential to develop an effective S. gallolyticus vaccine. Core proteomics was used in this study together with subtractive proteomics and reverse vaccinology approach to find antigenic proteins that could be utilized for the design of the S. gallolyticus multi-epitope vaccine. The pipeline identified two antigenic proteins as potential vaccine targets: penicillin-binding protein and the ATP synthase subunit. T and B cell epitopes from the specific proteins were forecasted employing several immunoinformatics and bioinformatics resources. A vaccine (360 amino acids) was created using a combination of seven cytotoxic T cell lymphocyte (CTL), three helper T cell lymphocyte (HTL), and five linear B cell lymphocyte (LBL) epitopes. To increase immune responses, the vaccine was paired with a cholera enterotoxin subunit B (CTB) adjuvant. The developed vaccine was highly antigenic, non-allergenic, and stable for human use. The vaccine's binding affinity and molecular interactions with the human immunological receptor TLR4 were studied using molecular mechanics/generalized Born surface area (MMGBSA), molecular docking, and molecular dynamic (MD) simulation analyses. Escherichia coli (strain K12) plasmid vector pET-28a ( +) was used to examine the ability of the vaccine to be expressed. According to the outcomes of these computer experiments, the vaccine is quite promising in terms of developing a protective immunity against diseases. However, in vitro and animal research are required to validate our findings.https://doi.org/10.1038/s41598-024-55372-3ImmunoinformaticsReverse vaccinologyPan-genomeMulti-epitope vaccine |
| spellingShingle | Peng Chao Xueqin Zhang Lei Zhang Aiping Yang Yong Wang Xiaoyang Chen Proteomics-based vaccine targets annotation and design of multi-epitope vaccine against antibiotic-resistant Streptococcus gallolyticus Scientific Reports Immunoinformatics Reverse vaccinology Pan-genome Multi-epitope vaccine |
| title | Proteomics-based vaccine targets annotation and design of multi-epitope vaccine against antibiotic-resistant Streptococcus gallolyticus |
| title_full | Proteomics-based vaccine targets annotation and design of multi-epitope vaccine against antibiotic-resistant Streptococcus gallolyticus |
| title_fullStr | Proteomics-based vaccine targets annotation and design of multi-epitope vaccine against antibiotic-resistant Streptococcus gallolyticus |
| title_full_unstemmed | Proteomics-based vaccine targets annotation and design of multi-epitope vaccine against antibiotic-resistant Streptococcus gallolyticus |
| title_short | Proteomics-based vaccine targets annotation and design of multi-epitope vaccine against antibiotic-resistant Streptococcus gallolyticus |
| title_sort | proteomics based vaccine targets annotation and design of multi epitope vaccine against antibiotic resistant streptococcus gallolyticus |
| topic | Immunoinformatics Reverse vaccinology Pan-genome Multi-epitope vaccine |
| url | https://doi.org/10.1038/s41598-024-55372-3 |
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