Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in <i>Citrus reticulate</i> ‘Chachi’ Peel
Portable and low-cost analytical devices are essential for rapid detection of bioactive substrates in agricultural products. This study presents the first highly integrated microelectrochemical sensor based on pencil graphite for rapid and sensitive detection of hesperidin in <i>Citrus reticul...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/14/5316 |
| _version_ | 1827732218316849152 |
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| author | Hong-Qi Xia Wanbing Chen Diyang Qiu Jiwu Zeng |
| author_facet | Hong-Qi Xia Wanbing Chen Diyang Qiu Jiwu Zeng |
| author_sort | Hong-Qi Xia |
| collection | DOAJ |
| description | Portable and low-cost analytical devices are essential for rapid detection of bioactive substrates in agricultural products. This study presents the first highly integrated microelectrochemical sensor based on pencil graphite for rapid and sensitive detection of hesperidin in <i>Citrus reticulate</i> ‘Chachi’ peel. The surface morphology and characterization as well as the electrochemical property of pencil graphite was investigated and discussed. A high electrocatalytic efficiency of hesperidin has been found at used pencil graphite-based microelectrodes. Kinetic analysis was carried out to further understand the electrochemical process of hesperidin at a pencil graphite microelectrode. Consequently, a portable and highly-integrated microelectrochemical sensor exhibits a sensitivity of 0.7251 μA cm<sup>−2</sup> μM<sup>−1</sup> and a detection limit as low as 25 nM (S/N = 3), and high selectivity was fabricated. Proposed microelectrochemical sensors were applied to electrochemically determinate the hesperidin content in the extract of <i>Citrus reticulata</i> “chachi” peel. As a result, the concentration of hesperidin in the actual real sample detected electrochemically with the proposed portable and low-cost microelectrochemical sensors is highly consistent to that obtained with a common chromatographic method, thus indicating the good reliability and that it can be used in practical applications. |
| first_indexed | 2024-03-11T00:47:31Z |
| format | Article |
| id | doaj.art-05805573b6a74a2daf8d4d122d4b4f16 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:47:31Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-05805573b6a74a2daf8d4d122d4b4f162023-11-18T20:40:18ZengMDPI AGMolecules1420-30492023-07-012814531610.3390/molecules28145316Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in <i>Citrus reticulate</i> ‘Chachi’ PeelHong-Qi Xia0Wanbing Chen1Diyang Qiu2Jiwu Zeng3Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (MARA), Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaKey Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (MARA), Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaKey Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (MARA), Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaKey Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (MARA), Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaPortable and low-cost analytical devices are essential for rapid detection of bioactive substrates in agricultural products. This study presents the first highly integrated microelectrochemical sensor based on pencil graphite for rapid and sensitive detection of hesperidin in <i>Citrus reticulate</i> ‘Chachi’ peel. The surface morphology and characterization as well as the electrochemical property of pencil graphite was investigated and discussed. A high electrocatalytic efficiency of hesperidin has been found at used pencil graphite-based microelectrodes. Kinetic analysis was carried out to further understand the electrochemical process of hesperidin at a pencil graphite microelectrode. Consequently, a portable and highly-integrated microelectrochemical sensor exhibits a sensitivity of 0.7251 μA cm<sup>−2</sup> μM<sup>−1</sup> and a detection limit as low as 25 nM (S/N = 3), and high selectivity was fabricated. Proposed microelectrochemical sensors were applied to electrochemically determinate the hesperidin content in the extract of <i>Citrus reticulata</i> “chachi” peel. As a result, the concentration of hesperidin in the actual real sample detected electrochemically with the proposed portable and low-cost microelectrochemical sensors is highly consistent to that obtained with a common chromatographic method, thus indicating the good reliability and that it can be used in practical applications.https://www.mdpi.com/1420-3049/28/14/5316microelectrochemical sensorhesperidin<i>Citrus reticulata</i> “chachi” |
| spellingShingle | Hong-Qi Xia Wanbing Chen Diyang Qiu Jiwu Zeng Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in <i>Citrus reticulate</i> ‘Chachi’ Peel Molecules microelectrochemical sensor hesperidin <i>Citrus reticulata</i> “chachi” |
| title | Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in <i>Citrus reticulate</i> ‘Chachi’ Peel |
| title_full | Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in <i>Citrus reticulate</i> ‘Chachi’ Peel |
| title_fullStr | Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in <i>Citrus reticulate</i> ‘Chachi’ Peel |
| title_full_unstemmed | Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in <i>Citrus reticulate</i> ‘Chachi’ Peel |
| title_short | Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in <i>Citrus reticulate</i> ‘Chachi’ Peel |
| title_sort | portable microelectrochemical sensors for rapid and sensitive determination of hesperidin in i citrus reticulate i chachi peel |
| topic | microelectrochemical sensor hesperidin <i>Citrus reticulata</i> “chachi” |
| url | https://www.mdpi.com/1420-3049/28/14/5316 |
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