A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation
The increasing rate of antimicrobial resistance (AMR) in pathogenic bacteria is a global threat to human and veterinary medicine. Beyond antibiotics, antimicrobial peptides (AMPs) might be an alternative to inhibit the growth of bacteria, including AMR pathogens, on different surfaces. Biofilm forma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/21/8/2771 |
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| author | Tobias Wieland Julia Assmann Astrid Bethe Christian Fidelak Helena Gmoser Traute Janßen Krishan Kotthaus Antina Lübke-Becker Lothar H. Wieler Gerald A. Urban |
| author_facet | Tobias Wieland Julia Assmann Astrid Bethe Christian Fidelak Helena Gmoser Traute Janßen Krishan Kotthaus Antina Lübke-Becker Lothar H. Wieler Gerald A. Urban |
| author_sort | Tobias Wieland |
| collection | DOAJ |
| description | The increasing rate of antimicrobial resistance (AMR) in pathogenic bacteria is a global threat to human and veterinary medicine. Beyond antibiotics, antimicrobial peptides (AMPs) might be an alternative to inhibit the growth of bacteria, including AMR pathogens, on different surfaces. Biofilm formation, which starts out as bacterial adhesion, poses additional challenges for antibiotics targeting bacterial cells. The objective of this study was to establish a real-time method for the monitoring of the inhibition of (a) bacterial adhesion to a defined substrate and (b) biofilm formation by AMPs using an innovative thermal sensor. We provide evidence that the thermal sensor enables continuous monitoring of the effect of two potent AMPs, protamine and OH-CATH-30, on surface colonization of bovine mastitis-associated <i>Escherichia</i> (<i>E.</i>) <i>coli</i> and <i>Staphylococcus</i> (<i>S.</i>) <i>aureus</i>. The bacteria were grown under static conditions on the surface of the sensor membrane, on which temperature oscillations generated by a heater structure were detected by an amorphous germanium thermistor. Bacterial adhesion, which was confirmed by white light interferometry, caused a detectable amplitude change and phase shift. To our knowledge, the thermal measurement system has never been used to assess the effect of AMPs on bacterial adhesion in real time before. The system could be used to screen and evaluate bacterial adhesion inhibition of both known and novel AMPs. |
| first_indexed | 2024-03-10T12:19:10Z |
| format | Article |
| id | doaj.art-0582db2cb0f545cea8d599e8ce67afec |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T12:19:10Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-0582db2cb0f545cea8d599e8ce67afec2023-11-21T15:35:31ZengMDPI AGSensors1424-82202021-04-01218277110.3390/s21082771A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm FormationTobias Wieland0Julia Assmann1Astrid Bethe2Christian Fidelak3Helena Gmoser4Traute Janßen5Krishan Kotthaus6Antina Lübke-Becker7Lothar H. Wieler8Gerald A. Urban9Department of Microsystems Engineering (IMTEK)—Laboratory of Sensors, University of Freiburg, 79110 Freiburg, GermanyInstitute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, GermanyInstitute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, GermanyBovicare GmbH, 14473 Potsdam, GermanyDepartment of Microsystems Engineering (IMTEK)—Laboratory of Sensors, University of Freiburg, 79110 Freiburg, GermanyRIPAC-Labor GmbH, 14476 Potsdam-Golm, GermanyDepartment of Microsystems Engineering (IMTEK)—Laboratory of Sensors, University of Freiburg, 79110 Freiburg, GermanyInstitute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, GermanyInstitute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, GermanyDepartment of Microsystems Engineering (IMTEK)—Laboratory of Sensors, University of Freiburg, 79110 Freiburg, GermanyThe increasing rate of antimicrobial resistance (AMR) in pathogenic bacteria is a global threat to human and veterinary medicine. Beyond antibiotics, antimicrobial peptides (AMPs) might be an alternative to inhibit the growth of bacteria, including AMR pathogens, on different surfaces. Biofilm formation, which starts out as bacterial adhesion, poses additional challenges for antibiotics targeting bacterial cells. The objective of this study was to establish a real-time method for the monitoring of the inhibition of (a) bacterial adhesion to a defined substrate and (b) biofilm formation by AMPs using an innovative thermal sensor. We provide evidence that the thermal sensor enables continuous monitoring of the effect of two potent AMPs, protamine and OH-CATH-30, on surface colonization of bovine mastitis-associated <i>Escherichia</i> (<i>E.</i>) <i>coli</i> and <i>Staphylococcus</i> (<i>S.</i>) <i>aureus</i>. The bacteria were grown under static conditions on the surface of the sensor membrane, on which temperature oscillations generated by a heater structure were detected by an amorphous germanium thermistor. Bacterial adhesion, which was confirmed by white light interferometry, caused a detectable amplitude change and phase shift. To our knowledge, the thermal measurement system has never been used to assess the effect of AMPs on bacterial adhesion in real time before. The system could be used to screen and evaluate bacterial adhesion inhibition of both known and novel AMPs.https://www.mdpi.com/1424-8220/21/8/2771thermal biosensorAMPsmeasurement in real timewhite light interferometry |
| spellingShingle | Tobias Wieland Julia Assmann Astrid Bethe Christian Fidelak Helena Gmoser Traute Janßen Krishan Kotthaus Antina Lübke-Becker Lothar H. Wieler Gerald A. Urban A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation Sensors thermal biosensor AMPs measurement in real time white light interferometry |
| title | A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation |
| title_full | A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation |
| title_fullStr | A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation |
| title_full_unstemmed | A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation |
| title_short | A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation |
| title_sort | real time thermal sensor system for quantifying the inhibitory effect of antimicrobial peptides on bacterial adhesion and biofilm formation |
| topic | thermal biosensor AMPs measurement in real time white light interferometry |
| url | https://www.mdpi.com/1424-8220/21/8/2771 |
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