Transcriptional profiling of <it>Medicago truncatula </it>under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses
<p>Abstract</p> <p>Background</p> <p>Salt stress hinders the growth of plants and reduces crop production worldwide. However, different plant species might possess different adaptive mechanisms to mitigate salt stress. We conducted a detailed pathway analysis of transcr...
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| Format: | Article |
| Language: | English |
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BMC
2011-07-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/11/109 |
| _version_ | 1811311390957240320 |
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| author | Su Zhen Ma Lei Shen Xiaoye Hu Xiaona Zhang Yunqin Li Daofeng Wang Tao Dong Jiangli |
| author_facet | Su Zhen Ma Lei Shen Xiaoye Hu Xiaona Zhang Yunqin Li Daofeng Wang Tao Dong Jiangli |
| author_sort | Su Zhen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Salt stress hinders the growth of plants and reduces crop production worldwide. However, different plant species might possess different adaptive mechanisms to mitigate salt stress. We conducted a detailed pathway analysis of transcriptional dynamics in the roots of <it>Medicago truncatula </it>seedlings under salt stress and selected a transcription factor gene, <it>MtCBF4</it>, for experimental validation.</p> <p>Results</p> <p>A microarray experiment was conducted using root samples collected 6, 24, and 48 h after application of 180 mM NaCl. Analysis of 11 statistically significant expression profiles revealed different behaviors between primary and secondary metabolism pathways in response to external stress. Secondary metabolism that helps to maintain osmotic balance was induced. One of the highly induced transcription factor genes was successfully cloned, and was named <it>MtCBF4</it>. Phylogenetic analysis revealed that MtCBF4, which belongs to the AP2-EREBP transcription factor family, is a novel member of the CBF transcription factor in <it>M. truncatula</it>. MtCBF4 is shown to be a nuclear-localized protein. Expression of <it>MtCBF4 </it>in <it>M. truncatula </it>was induced by most of the abiotic stresses, including salt, drought, cold, and abscisic acid, suggesting crosstalk between these abiotic stresses. Transgenic <it>Arabidopsis </it>over-expressing <it>MtCBF4 </it>enhanced tolerance to drought and salt stress, and activated expression of downstream genes that contain DRE elements. Over-expression of <it>MtCBF4 </it>in <it>M. truncatula </it>also enhanced salt tolerance and induced expression level of corresponding downstream genes.</p> <p>Conclusion</p> <p>Comprehensive transcriptomic analysis revealed complex mechanisms exist in plants in response to salt stress. The novel transcription factor gene <it>MtCBF4 </it>identified here played an important role in response to abiotic stresses, indicating that it might be a good candidate gene for genetic improvement to produce stress-tolerant plants.</p> |
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| issn | 1471-2229 |
| language | English |
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| spelling | doaj.art-0583616f53d443bb853127640556e3512022-12-22T02:50:39ZengBMCBMC Plant Biology1471-22292011-07-0111110910.1186/1471-2229-11-109Transcriptional profiling of <it>Medicago truncatula </it>under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responsesSu ZhenMa LeiShen XiaoyeHu XiaonaZhang YunqinLi DaofengWang TaoDong Jiangli<p>Abstract</p> <p>Background</p> <p>Salt stress hinders the growth of plants and reduces crop production worldwide. However, different plant species might possess different adaptive mechanisms to mitigate salt stress. We conducted a detailed pathway analysis of transcriptional dynamics in the roots of <it>Medicago truncatula </it>seedlings under salt stress and selected a transcription factor gene, <it>MtCBF4</it>, for experimental validation.</p> <p>Results</p> <p>A microarray experiment was conducted using root samples collected 6, 24, and 48 h after application of 180 mM NaCl. Analysis of 11 statistically significant expression profiles revealed different behaviors between primary and secondary metabolism pathways in response to external stress. Secondary metabolism that helps to maintain osmotic balance was induced. One of the highly induced transcription factor genes was successfully cloned, and was named <it>MtCBF4</it>. Phylogenetic analysis revealed that MtCBF4, which belongs to the AP2-EREBP transcription factor family, is a novel member of the CBF transcription factor in <it>M. truncatula</it>. MtCBF4 is shown to be a nuclear-localized protein. Expression of <it>MtCBF4 </it>in <it>M. truncatula </it>was induced by most of the abiotic stresses, including salt, drought, cold, and abscisic acid, suggesting crosstalk between these abiotic stresses. Transgenic <it>Arabidopsis </it>over-expressing <it>MtCBF4 </it>enhanced tolerance to drought and salt stress, and activated expression of downstream genes that contain DRE elements. Over-expression of <it>MtCBF4 </it>in <it>M. truncatula </it>also enhanced salt tolerance and induced expression level of corresponding downstream genes.</p> <p>Conclusion</p> <p>Comprehensive transcriptomic analysis revealed complex mechanisms exist in plants in response to salt stress. The novel transcription factor gene <it>MtCBF4 </it>identified here played an important role in response to abiotic stresses, indicating that it might be a good candidate gene for genetic improvement to produce stress-tolerant plants.</p>http://www.biomedcentral.com/1471-2229/11/109 |
| spellingShingle | Su Zhen Ma Lei Shen Xiaoye Hu Xiaona Zhang Yunqin Li Daofeng Wang Tao Dong Jiangli Transcriptional profiling of <it>Medicago truncatula </it>under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses BMC Plant Biology |
| title | Transcriptional profiling of <it>Medicago truncatula </it>under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses |
| title_full | Transcriptional profiling of <it>Medicago truncatula </it>under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses |
| title_fullStr | Transcriptional profiling of <it>Medicago truncatula </it>under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses |
| title_full_unstemmed | Transcriptional profiling of <it>Medicago truncatula </it>under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses |
| title_short | Transcriptional profiling of <it>Medicago truncatula </it>under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses |
| title_sort | transcriptional profiling of it medicago truncatula it under salt stress identified a novel cbf transcription factor mtcbf4 that plays an important role in abiotic stress responses |
| url | http://www.biomedcentral.com/1471-2229/11/109 |
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