Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates
Abstract Background Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infection (UTI); however, treatment of UTI has been challenging due to increased antimicrobial resistance (AMR). One of the most important types of AMR is carbapenem resistance (CR). CR bacteria are known as...
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BMC
2021-08-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
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| Online Access: | https://doi.org/10.1186/s12941-021-00457-4 |
| _version_ | 1818694238874894336 |
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| author | Fatemeh Zangane Matin Seyedeh Elham Rezatofighi Mohammad Roayaei Ardakani Mohammad Reza Akhoond Fahimeh Mahmoodi |
| author_facet | Fatemeh Zangane Matin Seyedeh Elham Rezatofighi Mohammad Roayaei Ardakani Mohammad Reza Akhoond Fahimeh Mahmoodi |
| author_sort | Fatemeh Zangane Matin |
| collection | DOAJ |
| description | Abstract Background Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infection (UTI); however, treatment of UTI has been challenging due to increased antimicrobial resistance (AMR). One of the most important types of AMR is carbapenem resistance (CR). CR bacteria are known as an important threat to global public health today. Class B metallo-beta-lactamases (MBLs) are one of the major factors for resistance against carbapenems. We aimed to investigate the characteristics of UPEC isolates producing MBL. Methods A cross-sectional study was conducted from October 2018 to December 2019 in Ahvaz; Iran. UPEC isolates were identified by biochemical and molecular methods. Metallo-beta-lactamase-producing isolates were detected using modified carbapenem inactivation method (mCIM) and EDTA-CIM (eCIM) tests. MBL genes, phylogenetic group, and virulence genes profile of carbapenem resistant isolates were determined. Conjugation assay and plasmid profiling were conducted to evaluate the ability of transferring of CR to other E. coli isolates. Clonal similarity of isolates were assessed using Enterobacterial intergenic repetitive element sequence (ERIC)-PCR. Results Among 406 UPEC isolates, 12 (2.95%) carbapenem-resistant were detected of which 11 were phenotypically MBL-producing strains. Four isolates were resistant to all investigated antimicrobial agents and were considered possible pandrug-resistant (PDR). bla NDM, bla OXA-48, bla IMP-1, and bla IMP-2 genes were found in 9, 5, 1, and 1 isolates, respectively. Among 30 virulence genes investigated, the traT, fyuA followed by fimH, and iutA with the frequency of 8 (66.7%), 8 (66.7%), 7 (58.3%), and 7 (58.3%) were the most identified genes, respectively. Siderophore production was the main virulence trait among carbapenem-resistant UPEC isolates. Except for two, all other isolates showed weak to moderate virulence index. In all recovered isolates, CR was readily transmitted via plasmids to other isolates during conjugation experiments. Conclusion MBL and carbapenemase genes, especially bla NDM and bla OXA-48 are spreading rapidly among bacteria, which can be a threat to global public health. Therefore monitoring the emergence and dissemination of new AMR is necessary to continuously refine guidelines for empiric antimicrobial therapy. Understanding the mechanisms of resistance and virulence in this group of bacteria can play an effective role in providing new therapeutic methods. |
| first_indexed | 2024-12-17T13:26:24Z |
| format | Article |
| id | doaj.art-058727889f044946a45d3c7f2ec815c3 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-12-17T13:26:24Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-058727889f044946a45d3c7f2ec815c32022-12-21T21:46:42ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112021-08-0120111310.1186/s12941-021-00457-4Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolatesFatemeh Zangane Matin0Seyedeh Elham Rezatofighi1Mohammad Roayaei Ardakani2Mohammad Reza Akhoond3Fahimeh Mahmoodi4Department of Biology, Faculty of Science, Shahid Chamran University of AhvazDepartment of Biology, Faculty of Science, Shahid Chamran University of AhvazDepartment of Biology, Faculty of Science, Shahid Chamran University of AhvazMathematical Sciences and Computer Faculty, Shahid Chamran University of AhvazDepartment of Biology, Faculty of Science, Shahid Chamran University of AhvazAbstract Background Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infection (UTI); however, treatment of UTI has been challenging due to increased antimicrobial resistance (AMR). One of the most important types of AMR is carbapenem resistance (CR). CR bacteria are known as an important threat to global public health today. Class B metallo-beta-lactamases (MBLs) are one of the major factors for resistance against carbapenems. We aimed to investigate the characteristics of UPEC isolates producing MBL. Methods A cross-sectional study was conducted from October 2018 to December 2019 in Ahvaz; Iran. UPEC isolates were identified by biochemical and molecular methods. Metallo-beta-lactamase-producing isolates were detected using modified carbapenem inactivation method (mCIM) and EDTA-CIM (eCIM) tests. MBL genes, phylogenetic group, and virulence genes profile of carbapenem resistant isolates were determined. Conjugation assay and plasmid profiling were conducted to evaluate the ability of transferring of CR to other E. coli isolates. Clonal similarity of isolates were assessed using Enterobacterial intergenic repetitive element sequence (ERIC)-PCR. Results Among 406 UPEC isolates, 12 (2.95%) carbapenem-resistant were detected of which 11 were phenotypically MBL-producing strains. Four isolates were resistant to all investigated antimicrobial agents and were considered possible pandrug-resistant (PDR). bla NDM, bla OXA-48, bla IMP-1, and bla IMP-2 genes were found in 9, 5, 1, and 1 isolates, respectively. Among 30 virulence genes investigated, the traT, fyuA followed by fimH, and iutA with the frequency of 8 (66.7%), 8 (66.7%), 7 (58.3%), and 7 (58.3%) were the most identified genes, respectively. Siderophore production was the main virulence trait among carbapenem-resistant UPEC isolates. Except for two, all other isolates showed weak to moderate virulence index. In all recovered isolates, CR was readily transmitted via plasmids to other isolates during conjugation experiments. Conclusion MBL and carbapenemase genes, especially bla NDM and bla OXA-48 are spreading rapidly among bacteria, which can be a threat to global public health. Therefore monitoring the emergence and dissemination of new AMR is necessary to continuously refine guidelines for empiric antimicrobial therapy. Understanding the mechanisms of resistance and virulence in this group of bacteria can play an effective role in providing new therapeutic methods.https://doi.org/10.1186/s12941-021-00457-4Uropathogenic Escherichia coliUrinary tract infectionMetallo-beta-lactamaseCarbapenemaseCarbapenem resistanceAntimicrobial resistance |
| spellingShingle | Fatemeh Zangane Matin Seyedeh Elham Rezatofighi Mohammad Roayaei Ardakani Mohammad Reza Akhoond Fahimeh Mahmoodi Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates Annals of Clinical Microbiology and Antimicrobials Uropathogenic Escherichia coli Urinary tract infection Metallo-beta-lactamase Carbapenemase Carbapenem resistance Antimicrobial resistance |
| title | Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates |
| title_full | Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates |
| title_fullStr | Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates |
| title_full_unstemmed | Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates |
| title_short | Virulence characterization and clonal analysis of uropathogenic Escherichia coli metallo-beta-lactamase-producing isolates |
| title_sort | virulence characterization and clonal analysis of uropathogenic escherichia coli metallo beta lactamase producing isolates |
| topic | Uropathogenic Escherichia coli Urinary tract infection Metallo-beta-lactamase Carbapenemase Carbapenem resistance Antimicrobial resistance |
| url | https://doi.org/10.1186/s12941-021-00457-4 |
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