Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis
INTRODUCTION: Domestic dogs are the most important reservoir in the peridomestic transmission cycle of Leishmania (Leishmania) chagasi. The genetic variability of subpopulations of this parasite circulating in dogs has not been thoroughly analyzed in Brazil, even though this knowledge has important...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2011-10-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500008&lng=en&tlng=en |
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| author | Carlos Eduardo Ribeiro Coutinho Dilvani Oliveira Santos Cibele Baptista Fabiano Borges Figueiredo Maria de Fatima Madeira |
| author_facet | Carlos Eduardo Ribeiro Coutinho Dilvani Oliveira Santos Cibele Baptista Fabiano Borges Figueiredo Maria de Fatima Madeira |
| author_sort | Carlos Eduardo Ribeiro Coutinho |
| collection | DOAJ |
| description | INTRODUCTION: Domestic dogs are the most important reservoir in the peridomestic transmission cycle of Leishmania (Leishmania) chagasi. The genetic variability of subpopulations of this parasite circulating in dogs has not been thoroughly analyzed in Brazil, even though this knowledge has important implications in the clinical-epidemiological context. METHODS: The objective of this study was to evaluate and compare the phenotypic variability of 153 L. chagasi strains isolated from dogs originating from the municipalities of Rio de Janeiro (n = 57) and Belo Horizonte (n = 96), where the disease is endemic. Strains isolated only from intact skin were selected and analyzed by multilocus enzyme electrophoresis using nine enzyme systems (6PG, GPI, NH1 and NH2, G6P, PGM, MDH, ME, and IDHNADP). RESULTS: The electrophoretic profile was identical for all isolates analyzed and was the same as that of the L. chagasi reference strain (MHOM/BR/74/PP75). Phenetic analysis showed a similarity index of one for all strains, with the isolates sharing 100% of the characteristics analyzed. CONCLUSIONS: The results demonstrate that the L. chagasi populations circulating in dogs from Rio de Janeiro and Belo Horizonte belong to a single zymodeme. |
| first_indexed | 2024-12-12T10:19:36Z |
| format | Article |
| id | doaj.art-0587c34eb32a4f3195b9dd2e7a745d36 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T10:19:36Z |
| publishDate | 2011-10-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-0587c34eb32a4f3195b9dd2e7a745d362022-12-22T00:27:35ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492011-10-0144557257510.1590/s0037-86822011005000049S0037-86822011000500008Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresisCarlos Eduardo Ribeiro Coutinho0Dilvani Oliveira Santos1Cibele Baptista2Fabiano Borges Figueiredo3Maria de Fatima Madeira4Fundação Oswaldo CruzUniversidade Federal FluminenseFundação Oswaldo CruzFundação Oswaldo CruzFundação Oswaldo CruzINTRODUCTION: Domestic dogs are the most important reservoir in the peridomestic transmission cycle of Leishmania (Leishmania) chagasi. The genetic variability of subpopulations of this parasite circulating in dogs has not been thoroughly analyzed in Brazil, even though this knowledge has important implications in the clinical-epidemiological context. METHODS: The objective of this study was to evaluate and compare the phenotypic variability of 153 L. chagasi strains isolated from dogs originating from the municipalities of Rio de Janeiro (n = 57) and Belo Horizonte (n = 96), where the disease is endemic. Strains isolated only from intact skin were selected and analyzed by multilocus enzyme electrophoresis using nine enzyme systems (6PG, GPI, NH1 and NH2, G6P, PGM, MDH, ME, and IDHNADP). RESULTS: The electrophoretic profile was identical for all isolates analyzed and was the same as that of the L. chagasi reference strain (MHOM/BR/74/PP75). Phenetic analysis showed a similarity index of one for all strains, with the isolates sharing 100% of the characteristics analyzed. CONCLUSIONS: The results demonstrate that the L. chagasi populations circulating in dogs from Rio de Janeiro and Belo Horizonte belong to a single zymodeme.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500008&lng=en&tlng=enCãoLeishmania chagasiVariabilidade genéticaMLEE |
| spellingShingle | Carlos Eduardo Ribeiro Coutinho Dilvani Oliveira Santos Cibele Baptista Fabiano Borges Figueiredo Maria de Fatima Madeira Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis Revista da Sociedade Brasileira de Medicina Tropical Cão Leishmania chagasi Variabilidade genética MLEE |
| title | Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
| title_full | Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
| title_fullStr | Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
| title_full_unstemmed | Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
| title_short | Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
| title_sort | evaluation of leishmania leishmania chagasi strains isolated from dogs originating from two visceral leishmaniasis endemic areas in brazil using multilocus enzyme electrophoresis |
| topic | Cão Leishmania chagasi Variabilidade genética MLEE |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500008&lng=en&tlng=en |
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