Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution
Surface plasmon resonance (SPR) is a powerful tool for determining molecular interactions quantitatively. SPR imaging (SPRi) further improves the throughput of SPR technology and provides the spatially resolved capability for observing the molecular interaction dynamics in detail. SPRi is becoming m...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/14/2/84 |
| _version_ | 1797298734273396736 |
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| author | Jiying Xu Pengfei Zhang Yi Chen |
| author_facet | Jiying Xu Pengfei Zhang Yi Chen |
| author_sort | Jiying Xu |
| collection | DOAJ |
| description | Surface plasmon resonance (SPR) is a powerful tool for determining molecular interactions quantitatively. SPR imaging (SPRi) further improves the throughput of SPR technology and provides the spatially resolved capability for observing the molecular interaction dynamics in detail. SPRi is becoming more and more popular in biological and chemical sensing and imaging. However, SPRi suffers from low spatial resolution due to the imperfect optical components and delocalized features of propagating surface plasmonic waves along the surface. Diverse kinds of approaches have been developed to improve the spatial resolution of SPRi, which have enormously impelled the development of the methodology and further extended its possible applications. In this minireview, we introduce the mechanisms for building a high-spatial-resolution SPRi system and present its experimental schemes from prism-coupled SPRi and SPR microscopy (SPRM) to surface plasmonic scattering microscopy (SPSM); summarize its exciting applications, including molecular interaction analysis, molecular imaging and profiling, tracking of single entities, and analysis of single cells; and discuss its challenges in recent decade as well as the promising future. |
| first_indexed | 2024-03-07T22:39:19Z |
| format | Article |
| id | doaj.art-0588d758ab4e40fd81383e3cda75235b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-07T22:39:19Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-0588d758ab4e40fd81383e3cda75235b2024-02-23T15:09:32ZengMDPI AGBiosensors2079-63742024-02-011428410.3390/bios14020084Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial ResolutionJiying Xu0Pengfei Zhang1Yi Chen2National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Faculty of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaKey Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, ChinaNational & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Faculty of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaSurface plasmon resonance (SPR) is a powerful tool for determining molecular interactions quantitatively. SPR imaging (SPRi) further improves the throughput of SPR technology and provides the spatially resolved capability for observing the molecular interaction dynamics in detail. SPRi is becoming more and more popular in biological and chemical sensing and imaging. However, SPRi suffers from low spatial resolution due to the imperfect optical components and delocalized features of propagating surface plasmonic waves along the surface. Diverse kinds of approaches have been developed to improve the spatial resolution of SPRi, which have enormously impelled the development of the methodology and further extended its possible applications. In this minireview, we introduce the mechanisms for building a high-spatial-resolution SPRi system and present its experimental schemes from prism-coupled SPRi and SPR microscopy (SPRM) to surface plasmonic scattering microscopy (SPSM); summarize its exciting applications, including molecular interaction analysis, molecular imaging and profiling, tracking of single entities, and analysis of single cells; and discuss its challenges in recent decade as well as the promising future.https://www.mdpi.com/2079-6374/14/2/84surface plasmon resonancesurface plasmon resonance imagingsurface plasmon resonance microscopysurface plasmonic scattering microscopymolecular imaginghigh spatial resolution |
| spellingShingle | Jiying Xu Pengfei Zhang Yi Chen Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution Biosensors surface plasmon resonance surface plasmon resonance imaging surface plasmon resonance microscopy surface plasmonic scattering microscopy molecular imaging high spatial resolution |
| title | Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution |
| title_full | Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution |
| title_fullStr | Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution |
| title_full_unstemmed | Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution |
| title_short | Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution |
| title_sort | surface plasmon resonance biosensors a review of molecular imaging with high spatial resolution |
| topic | surface plasmon resonance surface plasmon resonance imaging surface plasmon resonance microscopy surface plasmonic scattering microscopy molecular imaging high spatial resolution |
| url | https://www.mdpi.com/2079-6374/14/2/84 |
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