Orai1-mediated store-operated Ca2+ entry promotes cervical cancer progression through IL-6 signaling
Cervical cancer is a major cause of cancer-associated mortality among women in developing countries. Orai1-mediated store-operated Ca2+ entry (SOCE) is the primary mechanism underlying most of the non-excitable calcium influx into cells. There is at present limited evidence showing that Orai1 can fu...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.1041674/full |
| _version_ | 1811239125575008256 |
|---|---|
| author | Yiyun Pan Yiyun Pan Jing Huang Kang Liu Chuanhua Xie Hailong Chen Zhong Guo Shoujun Guo Yijian Chen Yijian Chen |
| author_facet | Yiyun Pan Yiyun Pan Jing Huang Kang Liu Chuanhua Xie Hailong Chen Zhong Guo Shoujun Guo Yijian Chen Yijian Chen |
| author_sort | Yiyun Pan |
| collection | DOAJ |
| description | Cervical cancer is a major cause of cancer-associated mortality among women in developing countries. Orai1-mediated store-operated Ca2+ entry (SOCE) is the primary mechanism underlying most of the non-excitable calcium influx into cells. There is at present limited evidence showing that Orai1 can function as an oncogene or a tumor suppressor depending on the cancer type. Furthermore, the exact biological functions of Orai1 in cervical cancer and the underlying mechanisms are still poorly understood. In this study, we found that Orai1 was upregulated in cervical cancer tissues, and promoted the growth of human cervical cancer cells both in vitro and in vivo. Gene silencing of Orai1 in cervical cancer cells significantly decreased interleukin (IL)-6 secretion. Interestingly, exogenous IL-6 abrogated the effects of Orai1 silencing and restored the clonogenicity of cervical cancer cells. Furthermore, we also observed a positive correlation between Orai1 and IL-6 expression in human cervical cancer samples. Taken together, our findings indicate that Orai1 functions as an oncogene in cervical cancer and is a promising therapeutic target. |
| first_indexed | 2024-04-12T12:54:21Z |
| format | Article |
| id | doaj.art-058a10d061ba4740981dfa0a55a61558 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-12T12:54:21Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-058a10d061ba4740981dfa0a55a615582022-12-22T03:32:21ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-10-01910.3389/fmolb.2022.10416741041674Orai1-mediated store-operated Ca2+ entry promotes cervical cancer progression through IL-6 signalingYiyun Pan0Yiyun Pan1Jing Huang2Kang Liu3Chuanhua Xie4Hailong Chen5Zhong Guo6Shoujun Guo7Yijian Chen8Yijian Chen9Suzhou Medical College of Soochow University, Suzhou, Jiangsu, ChinaGanzhou Cancer Hospital, Ganzhou, Jiangxi, ChinaGanzhou Cancer Hospital, Ganzhou, Jiangxi, ChinaThe First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaGanzhou Cancer Hospital, Ganzhou, Jiangxi, ChinaGanzhou Cancer Hospital, Ganzhou, Jiangxi, ChinaThe First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaGanzhou Cancer Hospital, Ganzhou, Jiangxi, ChinaSuzhou Medical College of Soochow University, Suzhou, Jiangsu, ChinaThe First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaCervical cancer is a major cause of cancer-associated mortality among women in developing countries. Orai1-mediated store-operated Ca2+ entry (SOCE) is the primary mechanism underlying most of the non-excitable calcium influx into cells. There is at present limited evidence showing that Orai1 can function as an oncogene or a tumor suppressor depending on the cancer type. Furthermore, the exact biological functions of Orai1 in cervical cancer and the underlying mechanisms are still poorly understood. In this study, we found that Orai1 was upregulated in cervical cancer tissues, and promoted the growth of human cervical cancer cells both in vitro and in vivo. Gene silencing of Orai1 in cervical cancer cells significantly decreased interleukin (IL)-6 secretion. Interestingly, exogenous IL-6 abrogated the effects of Orai1 silencing and restored the clonogenicity of cervical cancer cells. Furthermore, we also observed a positive correlation between Orai1 and IL-6 expression in human cervical cancer samples. Taken together, our findings indicate that Orai1 functions as an oncogene in cervical cancer and is a promising therapeutic target.https://www.frontiersin.org/articles/10.3389/fmolb.2022.1041674/fullOrai1SOCEcervical cancerIL-6progression |
| spellingShingle | Yiyun Pan Yiyun Pan Jing Huang Kang Liu Chuanhua Xie Hailong Chen Zhong Guo Shoujun Guo Yijian Chen Yijian Chen Orai1-mediated store-operated Ca2+ entry promotes cervical cancer progression through IL-6 signaling Frontiers in Molecular Biosciences Orai1 SOCE cervical cancer IL-6 progression |
| title | Orai1-mediated store-operated Ca2+ entry promotes cervical cancer progression through IL-6 signaling |
| title_full | Orai1-mediated store-operated Ca2+ entry promotes cervical cancer progression through IL-6 signaling |
| title_fullStr | Orai1-mediated store-operated Ca2+ entry promotes cervical cancer progression through IL-6 signaling |
| title_full_unstemmed | Orai1-mediated store-operated Ca2+ entry promotes cervical cancer progression through IL-6 signaling |
| title_short | Orai1-mediated store-operated Ca2+ entry promotes cervical cancer progression through IL-6 signaling |
| title_sort | orai1 mediated store operated ca2 entry promotes cervical cancer progression through il 6 signaling |
| topic | Orai1 SOCE cervical cancer IL-6 progression |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.1041674/full |
| work_keys_str_mv | AT yiyunpan orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling AT yiyunpan orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling AT jinghuang orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling AT kangliu orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling AT chuanhuaxie orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling AT hailongchen orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling AT zhongguo orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling AT shoujunguo orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling AT yijianchen orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling AT yijianchen orai1mediatedstoreoperatedca2entrypromotescervicalcancerprogressionthroughil6signaling |