Novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate
ObjectivesWhite spot lesions (WSLs) are prevalent and often lead to aesthetic problems and progressive caries. The objectives of this study were to: (1) develop a novel resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate (DMAEM) to inhibit WSLs, and (2) investigate the eff...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1063143/full |
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| author | Xiaoyu Huang Xiaoyu Huang Xiaoyu Huang Xiaoyu Huang Jingou Liang Jingou Liang Jingou Liang Wen Zhou Wen Zhou Wen Zhou Wen Zhou Tao Ma Michael D. Weir Gary D. Hack Guadalupe Garcia Fay Thomas W. Oates Lei Cheng Lei Cheng Hockin H. K. Xu Hockin H. K. Xu Hockin H. K. Xu |
| author_facet | Xiaoyu Huang Xiaoyu Huang Xiaoyu Huang Xiaoyu Huang Jingou Liang Jingou Liang Jingou Liang Wen Zhou Wen Zhou Wen Zhou Wen Zhou Tao Ma Michael D. Weir Gary D. Hack Guadalupe Garcia Fay Thomas W. Oates Lei Cheng Lei Cheng Hockin H. K. Xu Hockin H. K. Xu Hockin H. K. Xu |
| author_sort | Xiaoyu Huang |
| collection | DOAJ |
| description | ObjectivesWhite spot lesions (WSLs) are prevalent and often lead to aesthetic problems and progressive caries. The objectives of this study were to: (1) develop a novel resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate (DMAEM) to inhibit WSLs, and (2) investigate the effects of DMAEM incorporation on cytotoxicity, mechanical properties, biofilm-inhibition and protection of enamel hardness for the first time.MethodsDMAEM was synthesized using 1-bromododecane, 2-methylamino ethanol and methylmethacrylate. DMAEM with mass fractions of 0%, 1.25%, 2.5% and 5% were incorporated into a resin infiltant containing BisGMA and TEGDMA. Cytotoxicity, mechanical properties and antibacterial effects were tested. After resin infiltration, bovine enamel was demineralized with saliva biofilm acids, and enamel hardness was measured.ResultDMAEM infiltration did not increase the cytotoxicity or compromise the physical properties when DMAEM mass fraction was below 5% (p > 0.05). Biofilm metabolic activity was reduced by 90%, and biofilm lactic acid production was reduced by 92%, via DMAEM (p < 0.05). Mutans streptococci biofilm CFU was reduced by 3 logs (p < 0.05). When demineralized in acid and then under biofilms, the infiltrant + 5% DMAEM group produced an enamel hardness (mean ± sd; n = 6) of 2.90 ± 0.06 GPa, much higher than 0.85 ± 0.12 GPa of the infiltrant + 0% DMAEM group (p < 0.05).SignificanceA novel resin infiltrant with excellent mechanical properties, biocompability, strong antibacterial activity and anti-demineralization effect was developed using DMAEM for the first time. The DMAEM resin infiltrant is promising for inhibiting WSLs, arresting early caries, and protecting enamel hardness. |
| first_indexed | 2024-04-12T05:00:55Z |
| format | Article |
| id | doaj.art-058c2191a8e44e7a93f826aec806c05d |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-12T05:00:55Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-058c2191a8e44e7a93f826aec806c05d2022-12-22T03:46:59ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-11-011210.3389/fcimb.2022.10631431063143Novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylateXiaoyu Huang0Xiaoyu Huang1Xiaoyu Huang2Xiaoyu Huang3Jingou Liang4Jingou Liang5Jingou Liang6Wen Zhou7Wen Zhou8Wen Zhou9Wen Zhou10Tao Ma11Michael D. Weir12Gary D. Hack13Guadalupe Garcia Fay14Thomas W. Oates15Lei Cheng16Lei Cheng17Hockin H. K. Xu18Hockin H. K. Xu19Hockin H. K. Xu20State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, United StatesStomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, United StatesDepartment of Pediatric Dentistry, West China School of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, United StatesFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaDepartment of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD, United StatesDepartment of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, United StatesDepartment of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, United StatesDepartment of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, United StatesDepartment of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, United StatesState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, United StatesCenter for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD, United StatesMarlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD, United StatesObjectivesWhite spot lesions (WSLs) are prevalent and often lead to aesthetic problems and progressive caries. The objectives of this study were to: (1) develop a novel resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate (DMAEM) to inhibit WSLs, and (2) investigate the effects of DMAEM incorporation on cytotoxicity, mechanical properties, biofilm-inhibition and protection of enamel hardness for the first time.MethodsDMAEM was synthesized using 1-bromododecane, 2-methylamino ethanol and methylmethacrylate. DMAEM with mass fractions of 0%, 1.25%, 2.5% and 5% were incorporated into a resin infiltant containing BisGMA and TEGDMA. Cytotoxicity, mechanical properties and antibacterial effects were tested. After resin infiltration, bovine enamel was demineralized with saliva biofilm acids, and enamel hardness was measured.ResultDMAEM infiltration did not increase the cytotoxicity or compromise the physical properties when DMAEM mass fraction was below 5% (p > 0.05). Biofilm metabolic activity was reduced by 90%, and biofilm lactic acid production was reduced by 92%, via DMAEM (p < 0.05). Mutans streptococci biofilm CFU was reduced by 3 logs (p < 0.05). When demineralized in acid and then under biofilms, the infiltrant + 5% DMAEM group produced an enamel hardness (mean ± sd; n = 6) of 2.90 ± 0.06 GPa, much higher than 0.85 ± 0.12 GPa of the infiltrant + 0% DMAEM group (p < 0.05).SignificanceA novel resin infiltrant with excellent mechanical properties, biocompability, strong antibacterial activity and anti-demineralization effect was developed using DMAEM for the first time. The DMAEM resin infiltrant is promising for inhibiting WSLs, arresting early caries, and protecting enamel hardness.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1063143/fullquaternary ammoniumdodecylmethylaminoethyl methacrylatedental cariesresin infiltrantwhite spot lesion |
| spellingShingle | Xiaoyu Huang Xiaoyu Huang Xiaoyu Huang Xiaoyu Huang Jingou Liang Jingou Liang Jingou Liang Wen Zhou Wen Zhou Wen Zhou Wen Zhou Tao Ma Michael D. Weir Gary D. Hack Guadalupe Garcia Fay Thomas W. Oates Lei Cheng Lei Cheng Hockin H. K. Xu Hockin H. K. Xu Hockin H. K. Xu Novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate Frontiers in Cellular and Infection Microbiology quaternary ammonium dodecylmethylaminoethyl methacrylate dental caries resin infiltrant white spot lesion |
| title | Novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate |
| title_full | Novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate |
| title_fullStr | Novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate |
| title_full_unstemmed | Novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate |
| title_short | Novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate |
| title_sort | novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate |
| topic | quaternary ammonium dodecylmethylaminoethyl methacrylate dental caries resin infiltrant white spot lesion |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1063143/full |
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