High Metabolic Function of Primary Human and Porcine Hepatocytes in a Polyurethane Foam/Spheroid Culture System in Plasma from Patients with Fulminant Hepatic Failure
It has been demonstrated that plasma from patients with fulminant hepatic failure (FHF) interferes extensively with cellular function. We placed primary human and primary porcine hepatocytes in a polyurethane foam (PUF)/spheroid culture system and compared the metabolic functions in the plasma of pa...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2002-05-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000002783985855 |
| _version_ | 1818300199634731008 |
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| author | Yo-Ichi Yamashita M.D. Mitsuo Shimada Eiji Tsujita Ken Shirabe Hiroyuki Ijima Kohji Nakazawa Ryoichi Sakiyama Junji Fukuda Kazumori Funatsu Keizo Sugimachi |
| author_facet | Yo-Ichi Yamashita M.D. Mitsuo Shimada Eiji Tsujita Ken Shirabe Hiroyuki Ijima Kohji Nakazawa Ryoichi Sakiyama Junji Fukuda Kazumori Funatsu Keizo Sugimachi |
| author_sort | Yo-Ichi Yamashita M.D. |
| collection | DOAJ |
| description | It has been demonstrated that plasma from patients with fulminant hepatic failure (FHF) interferes extensively with cellular function. We placed primary human and primary porcine hepatocytes in a polyurethane foam (PUF)/spheroid culture system and compared the metabolic functions in the plasma of patients with FHF in a 24-h stationary culture to those in a monolayer culture. The PUF/spheroid culture system using primary human and primary porcine hepatocytes significantly decreased ammonia content during 28-day culture. Fisher's ratio significantly increased at culture days 3 and 7. Tauroursodeoxycholic acid significantly increased and glycochenodeoxycholic acid and taurochenodeoxycholic acid decreased in the FHF patients' plasma at culture day 3. During at least a 24-h culture in the FHF patients' plasma, metabolic functions of primary human and primary porcine hepatocytes were almost identical. The present results indicate that the PUF/spheroid culture system using primary human or primary porcine hepatocytes demonstrated more advantageous metabolic functions in the plasma from patients with FHF than the monolayer culture. |
| first_indexed | 2024-12-13T05:03:19Z |
| format | Article |
| id | doaj.art-058ceb6583274a72aa3e7edf9bb4af41 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-13T05:03:19Z |
| publishDate | 2002-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-058ceb6583274a72aa3e7edf9bb4af412022-12-21T23:58:43ZengSAGE PublishingCell Transplantation0963-68971555-38922002-05-011110.3727/000000002783985855High Metabolic Function of Primary Human and Porcine Hepatocytes in a Polyurethane Foam/Spheroid Culture System in Plasma from Patients with Fulminant Hepatic FailureYo-Ichi Yamashita M.D.0Mitsuo Shimada1Eiji Tsujita2Ken Shirabe3Hiroyuki Ijima4Kohji Nakazawa5Ryoichi Sakiyama6Junji Fukuda7Kazumori Funatsu8Keizo Sugimachi9Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Engineering, Graduate School of Engineering, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Engineering, Graduate School of Engineering, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Engineering, Graduate School of Engineering, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Engineering, Graduate School of Engineering, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Engineering, Graduate School of Engineering, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanIt has been demonstrated that plasma from patients with fulminant hepatic failure (FHF) interferes extensively with cellular function. We placed primary human and primary porcine hepatocytes in a polyurethane foam (PUF)/spheroid culture system and compared the metabolic functions in the plasma of patients with FHF in a 24-h stationary culture to those in a monolayer culture. The PUF/spheroid culture system using primary human and primary porcine hepatocytes significantly decreased ammonia content during 28-day culture. Fisher's ratio significantly increased at culture days 3 and 7. Tauroursodeoxycholic acid significantly increased and glycochenodeoxycholic acid and taurochenodeoxycholic acid decreased in the FHF patients' plasma at culture day 3. During at least a 24-h culture in the FHF patients' plasma, metabolic functions of primary human and primary porcine hepatocytes were almost identical. The present results indicate that the PUF/spheroid culture system using primary human or primary porcine hepatocytes demonstrated more advantageous metabolic functions in the plasma from patients with FHF than the monolayer culture.https://doi.org/10.3727/000000002783985855 |
| spellingShingle | Yo-Ichi Yamashita M.D. Mitsuo Shimada Eiji Tsujita Ken Shirabe Hiroyuki Ijima Kohji Nakazawa Ryoichi Sakiyama Junji Fukuda Kazumori Funatsu Keizo Sugimachi High Metabolic Function of Primary Human and Porcine Hepatocytes in a Polyurethane Foam/Spheroid Culture System in Plasma from Patients with Fulminant Hepatic Failure Cell Transplantation |
| title | High Metabolic Function of Primary Human and Porcine Hepatocytes in a Polyurethane Foam/Spheroid Culture System in Plasma from Patients with Fulminant Hepatic Failure |
| title_full | High Metabolic Function of Primary Human and Porcine Hepatocytes in a Polyurethane Foam/Spheroid Culture System in Plasma from Patients with Fulminant Hepatic Failure |
| title_fullStr | High Metabolic Function of Primary Human and Porcine Hepatocytes in a Polyurethane Foam/Spheroid Culture System in Plasma from Patients with Fulminant Hepatic Failure |
| title_full_unstemmed | High Metabolic Function of Primary Human and Porcine Hepatocytes in a Polyurethane Foam/Spheroid Culture System in Plasma from Patients with Fulminant Hepatic Failure |
| title_short | High Metabolic Function of Primary Human and Porcine Hepatocytes in a Polyurethane Foam/Spheroid Culture System in Plasma from Patients with Fulminant Hepatic Failure |
| title_sort | high metabolic function of primary human and porcine hepatocytes in a polyurethane foam spheroid culture system in plasma from patients with fulminant hepatic failure |
| url | https://doi.org/10.3727/000000002783985855 |
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