Molecular Analysis of Immune-Escape Mutants of Hepatitis B Virus from Local Clinical Samples
Small hepatitis B surface antigen (sHBsAg) is used as a component of hepatitis B vaccine. Even though this vaccine is known to be effective in preventing hepatitis B disease, natural mutation may induce Hepatitis B Virus (HBV) to form immune-escape mutant. This mutant is not only capable of infectin...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Microbiology
2012-04-01
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| Series: | Microbiology Indonesia |
| Subjects: | |
| Online Access: | https://jurnal.permi.or.id/index.php/mionline/article/view/109 |
| _version_ | 1819092850073141248 |
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| author | CHANDRA JINATA ERNAWATI ARIFIN GIRI-RACHMAN DEBBIE SOEFIE RETNONINGRUM |
| author_facet | CHANDRA JINATA ERNAWATI ARIFIN GIRI-RACHMAN DEBBIE SOEFIE RETNONINGRUM |
| author_sort | CHANDRA JINATA |
| collection | DOAJ |
| description | Small hepatitis B surface antigen (sHBsAg) is used as a component of hepatitis B vaccine. Even though this vaccine is known to be effective in preventing hepatitis B disease, natural mutation may induce Hepatitis B Virus (HBV) to form immune-escape mutant. This mutant is not only capable of infecting hepatitis B-vaccinated people, but also causing commercial diagnostic assay failure. Immune-escape mutant is generally detected from amino acid change at Major Hydrophilic Region (MHR) of sHBsAg while the change occurred outside the region may also lead to immune-escape mutant formation. This research was aimed to investigate the presence of HBV immune-escape mutants in local clinical samples in Indonesia. sHBsAg gene of seventeen HBV samples from local patients were amplified by polymerase chain reactions then subjected to two-directional sequencing. The DNA sequences later were analyzed by bioinformatics programs. Fifteen out of seventeen samples were genotype B and subtype adw2, while the other two were genotype C and subtype adrq+. Among fifteen genotype B samples, twelve of them were not immune-escape mutants, two were immune-escape mutants that have been previously reported (Gln129Arg and Met133Leu), and one was a mutant outside MHR that has not been previously reported as an immune-escape mutant (Tyr161Ser). Both samples of genotype C group were not immune-escape mutants. As conclusion, by investigating seventeen local clinical HBV samples, it was known that two of seventeen samples were confirmed as immune-escape mutants and one of seventeen samples was a mutant outside MHR. |
| first_indexed | 2024-12-21T23:02:10Z |
| format | Article |
| id | doaj.art-059214d0aed24290874b41d573a62cd1 |
| institution | Directory Open Access Journal |
| issn | 1978-3477 2087-8575 |
| language | English |
| last_indexed | 2024-12-21T23:02:10Z |
| publishDate | 2012-04-01 |
| publisher | Indonesian Society for Microbiology |
| record_format | Article |
| series | Microbiology Indonesia |
| spelling | doaj.art-059214d0aed24290874b41d573a62cd12022-12-21T18:47:16ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752012-04-016110.5454/mi.6.1.2Molecular Analysis of Immune-Escape Mutants of Hepatitis B Virus from Local Clinical SamplesCHANDRA JINATAERNAWATI ARIFIN GIRI-RACHMANDEBBIE SOEFIE RETNONINGRUMSmall hepatitis B surface antigen (sHBsAg) is used as a component of hepatitis B vaccine. Even though this vaccine is known to be effective in preventing hepatitis B disease, natural mutation may induce Hepatitis B Virus (HBV) to form immune-escape mutant. This mutant is not only capable of infecting hepatitis B-vaccinated people, but also causing commercial diagnostic assay failure. Immune-escape mutant is generally detected from amino acid change at Major Hydrophilic Region (MHR) of sHBsAg while the change occurred outside the region may also lead to immune-escape mutant formation. This research was aimed to investigate the presence of HBV immune-escape mutants in local clinical samples in Indonesia. sHBsAg gene of seventeen HBV samples from local patients were amplified by polymerase chain reactions then subjected to two-directional sequencing. The DNA sequences later were analyzed by bioinformatics programs. Fifteen out of seventeen samples were genotype B and subtype adw2, while the other two were genotype C and subtype adrq+. Among fifteen genotype B samples, twelve of them were not immune-escape mutants, two were immune-escape mutants that have been previously reported (Gln129Arg and Met133Leu), and one was a mutant outside MHR that has not been previously reported as an immune-escape mutant (Tyr161Ser). Both samples of genotype C group were not immune-escape mutants. As conclusion, by investigating seventeen local clinical HBV samples, it was known that two of seventeen samples were confirmed as immune-escape mutants and one of seventeen samples was a mutant outside MHR.https://jurnal.permi.or.id/index.php/mionline/article/view/109hepatitis B virusimmune-escape mutantmajor hydrophilic regionsmall hepatitis B surface antigen |
| spellingShingle | CHANDRA JINATA ERNAWATI ARIFIN GIRI-RACHMAN DEBBIE SOEFIE RETNONINGRUM Molecular Analysis of Immune-Escape Mutants of Hepatitis B Virus from Local Clinical Samples Microbiology Indonesia hepatitis B virus immune-escape mutant major hydrophilic region small hepatitis B surface antigen |
| title | Molecular Analysis of Immune-Escape Mutants of Hepatitis B Virus from Local Clinical Samples |
| title_full | Molecular Analysis of Immune-Escape Mutants of Hepatitis B Virus from Local Clinical Samples |
| title_fullStr | Molecular Analysis of Immune-Escape Mutants of Hepatitis B Virus from Local Clinical Samples |
| title_full_unstemmed | Molecular Analysis of Immune-Escape Mutants of Hepatitis B Virus from Local Clinical Samples |
| title_short | Molecular Analysis of Immune-Escape Mutants of Hepatitis B Virus from Local Clinical Samples |
| title_sort | molecular analysis of immune escape mutants of hepatitis b virus from local clinical samples |
| topic | hepatitis B virus immune-escape mutant major hydrophilic region small hepatitis B surface antigen |
| url | https://jurnal.permi.or.id/index.php/mionline/article/view/109 |
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