Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an Model of Osteoarthritis
Osteoarthritis (OA) is degenerative disease, leading to pain and functional disability. It is reported that polydeoxyribonucleotide (PDRN) is a suitable therapy for OA. However, the therapeutic mechanisms of PDRN in OA are not fully understood. To investigate the effect of PDRN in an in vitro model...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-11-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/0963689718804130 |
| _version_ | 1818402281537667072 |
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| author | Ahreum Baek Yoon Kim Jin Woo Lee Sang Chul Lee Sung-Rae Cho |
| author_facet | Ahreum Baek Yoon Kim Jin Woo Lee Sang Chul Lee Sung-Rae Cho |
| author_sort | Ahreum Baek |
| collection | DOAJ |
| description | Osteoarthritis (OA) is degenerative disease, leading to pain and functional disability. It is reported that polydeoxyribonucleotide (PDRN) is a suitable therapy for OA. However, the therapeutic mechanisms of PDRN in OA are not fully understood. To investigate the effect of PDRN in an in vitro model of OA, interleukin (IL)-1β or phosphate-buffered saline (PBS) was used to treat a human chondrocytic cell line in hypoxic conditions for 24 h (IL-1β group or control group). PDRN was then used to treat IL-1β group cells for 24 h (PDRN group). By Label-Based Human Antibody Array 1000, angiopoietin-2 (ANG-2), platelet-derived growth factor (PDGF), angiostatin, and endostatin, which were related to angiogenesis, were chosen for further validation studies. Quantitative real-time reverse transcription polymerase chain reaction and western blot analysis validated that the levels of PDGF and ANG-2, which were related to pro-angiogenesis, were significantly increased in the PDRN group compared with those in the control group or the IL-1β group. However, the levels of endostatin and angiostatin, which were related in anti-angiogenesis, were significantly decreased in the PDRN group compared with those in the control group or the IL-1β group. In the same manner, vascular endothelial growth factor, which was a mediator of angiogenesis, was significantly increased in the PDRN group compared with those in the control group or the IL-1β group. Furthermore, wound closure was significantly increased in the PDRN group compared with the control group or the IL-1β group by in vitro scratch assay. Moreover, PDRN decreased expression of metalloproteinase 13, as a catabolic factor for OA, but increased expression of aggrecan, which was an anabolic factor for OA. These data suggest that PDRN may promote angiogenesis and wound healing via down-regulation of catabolism and up-regulation of anabolism in an in vitro model of OA. |
| first_indexed | 2024-12-14T08:05:52Z |
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| id | doaj.art-059a68f8d076465d87df6fa368e1d91c |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-14T08:05:52Z |
| publishDate | 2018-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-059a68f8d076465d87df6fa368e1d91c2022-12-21T23:10:12ZengSAGE PublishingCell Transplantation0963-68971555-38922018-11-012710.1177/0963689718804130Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an Model of OsteoarthritisAhreum Baek0Yoon Kim1Jin Woo Lee2Sang Chul Lee3Sung-Rae Cho4 Department of Rehabilitation Medicine, Yonsei University Wonju College of Medicine, Wonju, South Korea Department of Physical and Rehabilitation Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul, South Korea Rehabilitation Institute of Neuromuscular Disease, Yonsei University College of Medicine, Seoul, South KoreaOsteoarthritis (OA) is degenerative disease, leading to pain and functional disability. It is reported that polydeoxyribonucleotide (PDRN) is a suitable therapy for OA. However, the therapeutic mechanisms of PDRN in OA are not fully understood. To investigate the effect of PDRN in an in vitro model of OA, interleukin (IL)-1β or phosphate-buffered saline (PBS) was used to treat a human chondrocytic cell line in hypoxic conditions for 24 h (IL-1β group or control group). PDRN was then used to treat IL-1β group cells for 24 h (PDRN group). By Label-Based Human Antibody Array 1000, angiopoietin-2 (ANG-2), platelet-derived growth factor (PDGF), angiostatin, and endostatin, which were related to angiogenesis, were chosen for further validation studies. Quantitative real-time reverse transcription polymerase chain reaction and western blot analysis validated that the levels of PDGF and ANG-2, which were related to pro-angiogenesis, were significantly increased in the PDRN group compared with those in the control group or the IL-1β group. However, the levels of endostatin and angiostatin, which were related in anti-angiogenesis, were significantly decreased in the PDRN group compared with those in the control group or the IL-1β group. In the same manner, vascular endothelial growth factor, which was a mediator of angiogenesis, was significantly increased in the PDRN group compared with those in the control group or the IL-1β group. Furthermore, wound closure was significantly increased in the PDRN group compared with the control group or the IL-1β group by in vitro scratch assay. Moreover, PDRN decreased expression of metalloproteinase 13, as a catabolic factor for OA, but increased expression of aggrecan, which was an anabolic factor for OA. These data suggest that PDRN may promote angiogenesis and wound healing via down-regulation of catabolism and up-regulation of anabolism in an in vitro model of OA.https://doi.org/10.1177/0963689718804130 |
| spellingShingle | Ahreum Baek Yoon Kim Jin Woo Lee Sang Chul Lee Sung-Rae Cho Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an Model of Osteoarthritis Cell Transplantation |
| title | Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an Model of Osteoarthritis |
| title_full | Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an Model of Osteoarthritis |
| title_fullStr | Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an Model of Osteoarthritis |
| title_full_unstemmed | Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an Model of Osteoarthritis |
| title_short | Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an Model of Osteoarthritis |
| title_sort | effect of polydeoxyribonucleotide on angiogenesis and wound healing in an model of osteoarthritis |
| url | https://doi.org/10.1177/0963689718804130 |
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