Identification of candidate genes responsible for chasmogamy in wheat
Abstract Background The flowering biology of wheat plants favours self-pollination which causes obstacles in wheat hybrid breeding. Wheat flowers can be divided into two groups, the first one is characterized by flowering and pollination within closed flowers (cleistogamy), while the second one poss...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-04-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-023-09252-1 |
| _version_ | 1797850141901717504 |
|---|---|
| author | Magdalena Szeliga Beata Bakera Magdalena Święcicka Mirosław Tyrka Monika Rakoczy-Trojanowska |
| author_facet | Magdalena Szeliga Beata Bakera Magdalena Święcicka Mirosław Tyrka Monika Rakoczy-Trojanowska |
| author_sort | Magdalena Szeliga |
| collection | DOAJ |
| description | Abstract Background The flowering biology of wheat plants favours self-pollination which causes obstacles in wheat hybrid breeding. Wheat flowers can be divided into two groups, the first one is characterized by flowering and pollination within closed flowers (cleistogamy), while the second one possesses the ability to open flowers during processes mentioned above (chasmogamy). The swelling of lodicules is involved in the flowering of cereals and among others their morphology, calcium and potassium content differentiate between cleistogamic and non-cleistogamous flowers. A better understanding of the chasmogamy mechanism can lead to the development of tools for selection of plants with the desired outcrossing rate. To learn more, the sequencing of transcriptomes (RNA-Seq) and Representational Difference Analysis products (RDA-Seq) were performed to investigate the global transcriptomes of wheat lodicules in two highly chasmogamous (HCH, Piko and Poezja) and two low chasmogamous (LCH, Euforia and KWS Dacanto) varieties at two developmental stages—pre-flowering and early flowering. Results The differentially expressed genes were enriched in five, main pathways: “metabolism”, “organismal systems”, “genetic information processing”, “cellular processes” and “environmental information processing”, respectively. Important genes with opposite patterns of regulation between the HCH and LCH lines have been associated with the lodicule development i.e. expression levels of MADS16 and MADS58 genes may be responsible for quantitative differences in chasmogamy level in wheat. Conclusions We conclude that the results provide a new insight into lodicules involvement in the wheat flowering process. This study generated important genomic information to support the exploitation of the chasmogamy in wheat hybrid breeding programs. |
| first_indexed | 2024-04-09T18:56:40Z |
| format | Article |
| id | doaj.art-059d29add09444278dbdcc58b644c1de |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-09T18:56:40Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-059d29add09444278dbdcc58b644c1de2023-04-09T11:09:01ZengBMCBMC Genomics1471-21642023-04-0124112110.1186/s12864-023-09252-1Identification of candidate genes responsible for chasmogamy in wheatMagdalena Szeliga0Beata Bakera1Magdalena Święcicka2Mirosław Tyrka3Monika Rakoczy-Trojanowska4Rzeszow University of TechnologyFaculty of Biology, Institute of Experimental Plant Biology and Biotechnology, University of WarsawWarsaw University of Life SciencesRzeszow University of TechnologyWarsaw University of Life SciencesAbstract Background The flowering biology of wheat plants favours self-pollination which causes obstacles in wheat hybrid breeding. Wheat flowers can be divided into two groups, the first one is characterized by flowering and pollination within closed flowers (cleistogamy), while the second one possesses the ability to open flowers during processes mentioned above (chasmogamy). The swelling of lodicules is involved in the flowering of cereals and among others their morphology, calcium and potassium content differentiate between cleistogamic and non-cleistogamous flowers. A better understanding of the chasmogamy mechanism can lead to the development of tools for selection of plants with the desired outcrossing rate. To learn more, the sequencing of transcriptomes (RNA-Seq) and Representational Difference Analysis products (RDA-Seq) were performed to investigate the global transcriptomes of wheat lodicules in two highly chasmogamous (HCH, Piko and Poezja) and two low chasmogamous (LCH, Euforia and KWS Dacanto) varieties at two developmental stages—pre-flowering and early flowering. Results The differentially expressed genes were enriched in five, main pathways: “metabolism”, “organismal systems”, “genetic information processing”, “cellular processes” and “environmental information processing”, respectively. Important genes with opposite patterns of regulation between the HCH and LCH lines have been associated with the lodicule development i.e. expression levels of MADS16 and MADS58 genes may be responsible for quantitative differences in chasmogamy level in wheat. Conclusions We conclude that the results provide a new insight into lodicules involvement in the wheat flowering process. This study generated important genomic information to support the exploitation of the chasmogamy in wheat hybrid breeding programs.https://doi.org/10.1186/s12864-023-09252-1Wheat breedingCleistogamyChasmogamyLodiculesRNA-SeqRDA-Seq |
| spellingShingle | Magdalena Szeliga Beata Bakera Magdalena Święcicka Mirosław Tyrka Monika Rakoczy-Trojanowska Identification of candidate genes responsible for chasmogamy in wheat BMC Genomics Wheat breeding Cleistogamy Chasmogamy Lodicules RNA-Seq RDA-Seq |
| title | Identification of candidate genes responsible for chasmogamy in wheat |
| title_full | Identification of candidate genes responsible for chasmogamy in wheat |
| title_fullStr | Identification of candidate genes responsible for chasmogamy in wheat |
| title_full_unstemmed | Identification of candidate genes responsible for chasmogamy in wheat |
| title_short | Identification of candidate genes responsible for chasmogamy in wheat |
| title_sort | identification of candidate genes responsible for chasmogamy in wheat |
| topic | Wheat breeding Cleistogamy Chasmogamy Lodicules RNA-Seq RDA-Seq |
| url | https://doi.org/10.1186/s12864-023-09252-1 |
| work_keys_str_mv | AT magdalenaszeliga identificationofcandidategenesresponsibleforchasmogamyinwheat AT beatabakera identificationofcandidategenesresponsibleforchasmogamyinwheat AT magdalenaswiecicka identificationofcandidategenesresponsibleforchasmogamyinwheat AT mirosławtyrka identificationofcandidategenesresponsibleforchasmogamyinwheat AT monikarakoczytrojanowska identificationofcandidategenesresponsibleforchasmogamyinwheat |