Differential and site specific impact of B cells in the protective immune response to Mycobacterium tuberculosis in the mouse.
Cell-mediated immune responses are known to be critical for control of mycobacterial infections whereas the role of B cells and humoral immunity is unclear. B cells can modulate immune responses by secretion of immunoglobulin, production of cytokines and antigen-presentation. To define the impact of...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3627912?pdf=render |
| _version_ | 1818271112571650048 |
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| author | Egídio Torrado Jeffrey J Fountain Richard T Robinson Cynthia A Martino John E Pearl Javier Rangel-Moreno Michael Tighe Robert Dunn Andrea M Cooper |
| author_facet | Egídio Torrado Jeffrey J Fountain Richard T Robinson Cynthia A Martino John E Pearl Javier Rangel-Moreno Michael Tighe Robert Dunn Andrea M Cooper |
| author_sort | Egídio Torrado |
| collection | DOAJ |
| description | Cell-mediated immune responses are known to be critical for control of mycobacterial infections whereas the role of B cells and humoral immunity is unclear. B cells can modulate immune responses by secretion of immunoglobulin, production of cytokines and antigen-presentation. To define the impact of B cells in the absence of secreted immunoglobulin, we analyzed the progression of Mycobacterium tuberculosis (Mtb) infection in mice that have B cells but which lack secretory immunoglobulin (AID(-/-)µS(-/-)mice). AID(-/-)µS(-/-) mice accumulated a population of activated B cells in the lungs when infected and were more susceptible to aerosol Mtb when compared to wild type (C57BL/6) mice or indeed mice that totally lack B cells. The enhanced susceptibility of AID(-/-)µS(-/-) mice was not associated with defective T cell activation or expression of a type 1 immune response. While delivery of normal serum to AID(-/-)µS(-/-) mice did not reverse susceptibility, susceptibility in the spleen was dependent upon the presence of B cells and susceptibility in the lungs of AID(-/-)µS(-/-)mice was associated with elevated expression of the cytokines IL-6, GM-CSF, IL-10 and molecules made by alternatively activated macrophages. Blocking of IL-10 signaling resulted in reversal of susceptibility in the spleens and lungs of AID(-/-)µS(-/-) mice. These data support the hypothesis that B cells can modulate immunity to Mtb in an organ specific manner via the modulation of cytokine production and macrophage activation. |
| first_indexed | 2024-12-12T21:21:00Z |
| format | Article |
| id | doaj.art-059d6ef93c0a4e7dbd6cc6dad33edcd3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T21:21:00Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-059d6ef93c0a4e7dbd6cc6dad33edcd32022-12-22T00:11:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6168110.1371/journal.pone.0061681Differential and site specific impact of B cells in the protective immune response to Mycobacterium tuberculosis in the mouse.Egídio TorradoJeffrey J FountainRichard T RobinsonCynthia A MartinoJohn E PearlJavier Rangel-MorenoMichael TigheRobert DunnAndrea M CooperCell-mediated immune responses are known to be critical for control of mycobacterial infections whereas the role of B cells and humoral immunity is unclear. B cells can modulate immune responses by secretion of immunoglobulin, production of cytokines and antigen-presentation. To define the impact of B cells in the absence of secreted immunoglobulin, we analyzed the progression of Mycobacterium tuberculosis (Mtb) infection in mice that have B cells but which lack secretory immunoglobulin (AID(-/-)µS(-/-)mice). AID(-/-)µS(-/-) mice accumulated a population of activated B cells in the lungs when infected and were more susceptible to aerosol Mtb when compared to wild type (C57BL/6) mice or indeed mice that totally lack B cells. The enhanced susceptibility of AID(-/-)µS(-/-) mice was not associated with defective T cell activation or expression of a type 1 immune response. While delivery of normal serum to AID(-/-)µS(-/-) mice did not reverse susceptibility, susceptibility in the spleen was dependent upon the presence of B cells and susceptibility in the lungs of AID(-/-)µS(-/-)mice was associated with elevated expression of the cytokines IL-6, GM-CSF, IL-10 and molecules made by alternatively activated macrophages. Blocking of IL-10 signaling resulted in reversal of susceptibility in the spleens and lungs of AID(-/-)µS(-/-) mice. These data support the hypothesis that B cells can modulate immunity to Mtb in an organ specific manner via the modulation of cytokine production and macrophage activation.http://europepmc.org/articles/PMC3627912?pdf=render |
| spellingShingle | Egídio Torrado Jeffrey J Fountain Richard T Robinson Cynthia A Martino John E Pearl Javier Rangel-Moreno Michael Tighe Robert Dunn Andrea M Cooper Differential and site specific impact of B cells in the protective immune response to Mycobacterium tuberculosis in the mouse. PLoS ONE |
| title | Differential and site specific impact of B cells in the protective immune response to Mycobacterium tuberculosis in the mouse. |
| title_full | Differential and site specific impact of B cells in the protective immune response to Mycobacterium tuberculosis in the mouse. |
| title_fullStr | Differential and site specific impact of B cells in the protective immune response to Mycobacterium tuberculosis in the mouse. |
| title_full_unstemmed | Differential and site specific impact of B cells in the protective immune response to Mycobacterium tuberculosis in the mouse. |
| title_short | Differential and site specific impact of B cells in the protective immune response to Mycobacterium tuberculosis in the mouse. |
| title_sort | differential and site specific impact of b cells in the protective immune response to mycobacterium tuberculosis in the mouse |
| url | http://europepmc.org/articles/PMC3627912?pdf=render |
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