PI3K Isoform-Specific Regulation of Leader and Follower Cell Function for Collective Migration and Proliferation in Response to Injury
To ensure proper wound healing it is important to elucidate the signaling cues that coordinate leader and follower cell behavior to promote collective migration and proliferation for wound healing in response to injury. Using an ex vivo post-cataract surgery wound healing model we investigated the r...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/21/3515 |
| _version_ | 1797468684819628032 |
|---|---|
| author | Morgan D. Basta A. Sue Menko Janice L. Walker |
| author_facet | Morgan D. Basta A. Sue Menko Janice L. Walker |
| author_sort | Morgan D. Basta |
| collection | DOAJ |
| description | To ensure proper wound healing it is important to elucidate the signaling cues that coordinate leader and follower cell behavior to promote collective migration and proliferation for wound healing in response to injury. Using an ex vivo post-cataract surgery wound healing model we investigated the role of class I phosphatidylinositol-3-kinase (PI3K) isoforms in this process. Our findings revealed a specific role for p110α signaling independent of Akt for promoting the collective migration and proliferation of the epithelium for wound closure. In addition, we found an important role for p110α signaling in orchestrating proper polarized cytoskeletal organization within both leader and wounded epithelial follower cells to coordinate their function for wound healing. p110α was necessary to signal the formation and persistence of vimentin rich-lamellipodia extensions by leader cells and the reorganization of actomyosin into stress fibers along the basal domains of the wounded lens epithelial follower cells for movement. Together, our study reveals a critical role for p110α in the collective migration of an epithelium in response to wounding. |
| first_indexed | 2024-03-09T19:11:02Z |
| format | Article |
| id | doaj.art-05a73ca23274436f93da6ec1974d5978 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T19:11:02Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-05a73ca23274436f93da6ec1974d59782023-11-24T04:10:03ZengMDPI AGCells2073-44092022-11-011121351510.3390/cells11213515PI3K Isoform-Specific Regulation of Leader and Follower Cell Function for Collective Migration and Proliferation in Response to InjuryMorgan D. Basta0A. Sue Menko1Janice L. Walker2Department of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USADepartment of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USADepartment of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USATo ensure proper wound healing it is important to elucidate the signaling cues that coordinate leader and follower cell behavior to promote collective migration and proliferation for wound healing in response to injury. Using an ex vivo post-cataract surgery wound healing model we investigated the role of class I phosphatidylinositol-3-kinase (PI3K) isoforms in this process. Our findings revealed a specific role for p110α signaling independent of Akt for promoting the collective migration and proliferation of the epithelium for wound closure. In addition, we found an important role for p110α signaling in orchestrating proper polarized cytoskeletal organization within both leader and wounded epithelial follower cells to coordinate their function for wound healing. p110α was necessary to signal the formation and persistence of vimentin rich-lamellipodia extensions by leader cells and the reorganization of actomyosin into stress fibers along the basal domains of the wounded lens epithelial follower cells for movement. Together, our study reveals a critical role for p110α in the collective migration of an epithelium in response to wounding.https://www.mdpi.com/2073-4409/11/21/3515collective migrationleader cellfollower cellwound healingPI3Kp110α |
| spellingShingle | Morgan D. Basta A. Sue Menko Janice L. Walker PI3K Isoform-Specific Regulation of Leader and Follower Cell Function for Collective Migration and Proliferation in Response to Injury Cells collective migration leader cell follower cell wound healing PI3K p110α |
| title | PI3K Isoform-Specific Regulation of Leader and Follower Cell Function for Collective Migration and Proliferation in Response to Injury |
| title_full | PI3K Isoform-Specific Regulation of Leader and Follower Cell Function for Collective Migration and Proliferation in Response to Injury |
| title_fullStr | PI3K Isoform-Specific Regulation of Leader and Follower Cell Function for Collective Migration and Proliferation in Response to Injury |
| title_full_unstemmed | PI3K Isoform-Specific Regulation of Leader and Follower Cell Function for Collective Migration and Proliferation in Response to Injury |
| title_short | PI3K Isoform-Specific Regulation of Leader and Follower Cell Function for Collective Migration and Proliferation in Response to Injury |
| title_sort | pi3k isoform specific regulation of leader and follower cell function for collective migration and proliferation in response to injury |
| topic | collective migration leader cell follower cell wound healing PI3K p110α |
| url | https://www.mdpi.com/2073-4409/11/21/3515 |
| work_keys_str_mv | AT morgandbasta pi3kisoformspecificregulationofleaderandfollowercellfunctionforcollectivemigrationandproliferationinresponsetoinjury AT asuemenko pi3kisoformspecificregulationofleaderandfollowercellfunctionforcollectivemigrationandproliferationinresponsetoinjury AT janicelwalker pi3kisoformspecificregulationofleaderandfollowercellfunctionforcollectivemigrationandproliferationinresponsetoinjury |