Hydrogel treatment for idiopathic osteoarthritis in a Dunkin Hartley Guinea pig model
The study objective was to determine if intraarticular injections of an extracellular matrix (ECM) powder and blood composite (ECM-B) would have a significant impact on post-operative gait parameters without eliciting adverse cartilage changes or severe lymphatic reactions in an idiopathic osteoarth...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710799/?tool=EBI |
| _version_ | 1811208107871698944 |
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| author | Lauren R. Parola Megan P. Pinette Benedikt L. Proffen Nicholas J. Sant N. Padmini Karamchedu Meggin Q. Costa Janine Molino Braden C. Fleming Martha M. Murray |
| author_facet | Lauren R. Parola Megan P. Pinette Benedikt L. Proffen Nicholas J. Sant N. Padmini Karamchedu Meggin Q. Costa Janine Molino Braden C. Fleming Martha M. Murray |
| author_sort | Lauren R. Parola |
| collection | DOAJ |
| description | The study objective was to determine if intraarticular injections of an extracellular matrix (ECM) powder and blood composite (ECM-B) would have a significant impact on post-operative gait parameters without eliciting adverse cartilage changes or severe lymphatic reactions in an idiopathic osteoarthritis (OA) model. Twenty-one Dunkin Hartley Guinea pigs received an intraarticular injection of ECM-B in each knee and were split into sub-groups for gait assessment and post-harvest knee evaluations at 1 week (n = 5), 2 weeks (n = 5), 4 weeks (n = 5), or 8 weeks (n = 6). The results were compared with a control group (n = 5), which underwent bilateral injections of phosphate-buffered saline (PBS), gait measurements at 1, 2, 4, and 8 weeks, and post-mortem knee evaluation at 8 weeks post-injection. Hind limbs and popliteal lymph nodes were collected at the Week 8 endpoint and underwent histological analysis by a veterinary pathologist. Significant improvement in hind limb base of support was observed in the ECM-B group compared to the control group at Week 4 but was no longer significant by Week 8. No significant differences were observed between control and ECM-B groups in hind limb cartilage, synovium, or popliteal lymph node histology at Week 8. In conclusion, administration of an ECM-B material may improve gait for a limited time without significant adverse effects on the cartilage, synovium, or local lymph nodes. |
| first_indexed | 2024-04-12T04:15:09Z |
| format | Article |
| id | doaj.art-05a90ca7345d451db818c89cc92bc628 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:15:09Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-05a90ca7345d451db818c89cc92bc6282022-12-22T03:48:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011711Hydrogel treatment for idiopathic osteoarthritis in a Dunkin Hartley Guinea pig modelLauren R. ParolaMegan P. PinetteBenedikt L. ProffenNicholas J. SantN. Padmini KaramcheduMeggin Q. CostaJanine MolinoBraden C. FlemingMartha M. MurrayThe study objective was to determine if intraarticular injections of an extracellular matrix (ECM) powder and blood composite (ECM-B) would have a significant impact on post-operative gait parameters without eliciting adverse cartilage changes or severe lymphatic reactions in an idiopathic osteoarthritis (OA) model. Twenty-one Dunkin Hartley Guinea pigs received an intraarticular injection of ECM-B in each knee and were split into sub-groups for gait assessment and post-harvest knee evaluations at 1 week (n = 5), 2 weeks (n = 5), 4 weeks (n = 5), or 8 weeks (n = 6). The results were compared with a control group (n = 5), which underwent bilateral injections of phosphate-buffered saline (PBS), gait measurements at 1, 2, 4, and 8 weeks, and post-mortem knee evaluation at 8 weeks post-injection. Hind limbs and popliteal lymph nodes were collected at the Week 8 endpoint and underwent histological analysis by a veterinary pathologist. Significant improvement in hind limb base of support was observed in the ECM-B group compared to the control group at Week 4 but was no longer significant by Week 8. No significant differences were observed between control and ECM-B groups in hind limb cartilage, synovium, or popliteal lymph node histology at Week 8. In conclusion, administration of an ECM-B material may improve gait for a limited time without significant adverse effects on the cartilage, synovium, or local lymph nodes.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710799/?tool=EBI |
| spellingShingle | Lauren R. Parola Megan P. Pinette Benedikt L. Proffen Nicholas J. Sant N. Padmini Karamchedu Meggin Q. Costa Janine Molino Braden C. Fleming Martha M. Murray Hydrogel treatment for idiopathic osteoarthritis in a Dunkin Hartley Guinea pig model PLoS ONE |
| title | Hydrogel treatment for idiopathic osteoarthritis in a Dunkin Hartley Guinea pig model |
| title_full | Hydrogel treatment for idiopathic osteoarthritis in a Dunkin Hartley Guinea pig model |
| title_fullStr | Hydrogel treatment for idiopathic osteoarthritis in a Dunkin Hartley Guinea pig model |
| title_full_unstemmed | Hydrogel treatment for idiopathic osteoarthritis in a Dunkin Hartley Guinea pig model |
| title_short | Hydrogel treatment for idiopathic osteoarthritis in a Dunkin Hartley Guinea pig model |
| title_sort | hydrogel treatment for idiopathic osteoarthritis in a dunkin hartley guinea pig model |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710799/?tool=EBI |
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