The protective potential and possible mechanism of Phyllanthus amarus Schum. & Thonn. aqueous extract on paracetamol-induced hepatotoxicity in rats
The hepatoprotective potential of Phyllanthus amarus Schum. & Thonn. was studied on paracetamolinduced hepatotoxicity in rats by measuring the levels of serum transaminase (SGOT and SGPT), alkaline phosphatase (ALP) and bilirubin, as well as by histopathological examination of the liver. Further...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2006-05-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/28_3_pdf/10-Phyllanthusamarus-Schum.pdf |
| _version_ | 1818342884589436928 |
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| author | Apichat Muso Vipavadee Prasartthong Anupong Nitiruangjaras Patchara Rattanapirun Peerarat Thaina Nisita Bumrungwong Malinee Wongnawa |
| author_facet | Apichat Muso Vipavadee Prasartthong Anupong Nitiruangjaras Patchara Rattanapirun Peerarat Thaina Nisita Bumrungwong Malinee Wongnawa |
| author_sort | Apichat Muso |
| collection | DOAJ |
| description | The hepatoprotective potential of Phyllanthus amarus Schum. & Thonn. was studied on paracetamolinduced hepatotoxicity in rats by measuring the levels of serum transaminase (SGOT and SGPT), alkaline phosphatase (ALP) and bilirubin, as well as by histopathological examination of the liver. Furthermore, the hepatoprotective mechanisms were investigated by determining the amount of paracetamol and its metabolites (glucuronide, sulfate, cysteine and mercapturic acid conjugates) in urine and pentobarbital-induced sleeping time to indicate the inhibition on cytochrome P450. The involvement of glutathione was evaluated by determining hepatic reduced glutathione. Its radical scavenging activity, iron chelating activity and total phenolic content were also determined. P. amarus aqueous extracts (0.8, 1.6 or 3.2 g/kg) were orally administered twice daily for 7 days prior, for 2 days after, or for 7 days prior and followed by 2 days after a single oral dose of paracetamol (3 g/kg). The results showed that the extract at the doses of 1.6 and 3.2 g/kg decreased the paracetamol-induced hepatotoxicity as indicated by the decrease in SGOT, SGPT, bilirubin and histopathological score while the ALP did not change. Moreover, it is suggested that the hepatoprotective mechanism of this plant was related neither to the inhibition on cytochrome P450, nor to the induction on sulfate and/or glucuronide conjugation pathways of paracetamol, but partly due to the protective effect on the depletion of hepatic reduced glutathione and also its antioxidant activity, especially the radical scavenging and iron chelating activity, which might be related to the high polyphenolic contents. These results support the value of P. amarus, which has been used in Thai folk medicine for the treatment of liver diseases. |
| first_indexed | 2024-12-13T16:21:47Z |
| format | Article |
| id | doaj.art-05ac8fe4dab04546bee423852a742170 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-13T16:21:47Z |
| publishDate | 2006-05-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-05ac8fe4dab04546bee423852a7421702022-12-21T23:38:42ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952006-05-01283551561The protective potential and possible mechanism of Phyllanthus amarus Schum. & Thonn. aqueous extract on paracetamol-induced hepatotoxicity in ratsApichat MusoVipavadee PrasartthongAnupong NitiruangjarasPatchara RattanapirunPeerarat ThainaNisita BumrungwongMalinee WongnawaThe hepatoprotective potential of Phyllanthus amarus Schum. & Thonn. was studied on paracetamolinduced hepatotoxicity in rats by measuring the levels of serum transaminase (SGOT and SGPT), alkaline phosphatase (ALP) and bilirubin, as well as by histopathological examination of the liver. Furthermore, the hepatoprotective mechanisms were investigated by determining the amount of paracetamol and its metabolites (glucuronide, sulfate, cysteine and mercapturic acid conjugates) in urine and pentobarbital-induced sleeping time to indicate the inhibition on cytochrome P450. The involvement of glutathione was evaluated by determining hepatic reduced glutathione. Its radical scavenging activity, iron chelating activity and total phenolic content were also determined. P. amarus aqueous extracts (0.8, 1.6 or 3.2 g/kg) were orally administered twice daily for 7 days prior, for 2 days after, or for 7 days prior and followed by 2 days after a single oral dose of paracetamol (3 g/kg). The results showed that the extract at the doses of 1.6 and 3.2 g/kg decreased the paracetamol-induced hepatotoxicity as indicated by the decrease in SGOT, SGPT, bilirubin and histopathological score while the ALP did not change. Moreover, it is suggested that the hepatoprotective mechanism of this plant was related neither to the inhibition on cytochrome P450, nor to the induction on sulfate and/or glucuronide conjugation pathways of paracetamol, but partly due to the protective effect on the depletion of hepatic reduced glutathione and also its antioxidant activity, especially the radical scavenging and iron chelating activity, which might be related to the high polyphenolic contents. These results support the value of P. amarus, which has been used in Thai folk medicine for the treatment of liver diseases.http://www.sjst.psu.ac.th/journal/28_3_pdf/10-Phyllanthusamarus-Schum.pdfPhyllanthus amarusparacetamolcytochrome P450glutathioneantioxidant activity |
| spellingShingle | Apichat Muso Vipavadee Prasartthong Anupong Nitiruangjaras Patchara Rattanapirun Peerarat Thaina Nisita Bumrungwong Malinee Wongnawa The protective potential and possible mechanism of Phyllanthus amarus Schum. & Thonn. aqueous extract on paracetamol-induced hepatotoxicity in rats Songklanakarin Journal of Science and Technology (SJST) Phyllanthus amarus paracetamol cytochrome P450 glutathione antioxidant activity |
| title | The protective potential and possible mechanism of Phyllanthus amarus Schum. & Thonn. aqueous extract on paracetamol-induced hepatotoxicity in rats |
| title_full | The protective potential and possible mechanism of Phyllanthus amarus Schum. & Thonn. aqueous extract on paracetamol-induced hepatotoxicity in rats |
| title_fullStr | The protective potential and possible mechanism of Phyllanthus amarus Schum. & Thonn. aqueous extract on paracetamol-induced hepatotoxicity in rats |
| title_full_unstemmed | The protective potential and possible mechanism of Phyllanthus amarus Schum. & Thonn. aqueous extract on paracetamol-induced hepatotoxicity in rats |
| title_short | The protective potential and possible mechanism of Phyllanthus amarus Schum. & Thonn. aqueous extract on paracetamol-induced hepatotoxicity in rats |
| title_sort | protective potential and possible mechanism of phyllanthus amarus schum thonn aqueous extract on paracetamol induced hepatotoxicity in rats |
| topic | Phyllanthus amarus paracetamol cytochrome P450 glutathione antioxidant activity |
| url | http://www.sjst.psu.ac.th/journal/28_3_pdf/10-Phyllanthusamarus-Schum.pdf |
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