Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients
Cerebrospinal fluid (CSF) samples from clinically diagnosed patients with detectable Angiostrongylus canto-nensis-specific antibodies (n = 10), patients with clinically suspected cases that tested negative for A. cantonensis-an-tibodies (n = 5) and patients with cerebral gnathostomiasis (n = 2) and...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2013-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100020&lng=en&tlng=en |
| _version_ | 1797703153951440896 |
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| author | Praphathip Eamsobhana Darawan Wanachiwanawin Naowarat Dechkum Anchana Parsartvit Hoi Sen Yong |
| author_facet | Praphathip Eamsobhana Darawan Wanachiwanawin Naowarat Dechkum Anchana Parsartvit Hoi Sen Yong |
| author_sort | Praphathip Eamsobhana |
| collection | DOAJ |
| description | Cerebrospinal fluid (CSF) samples from clinically diagnosed patients with detectable Angiostrongylus canto-nensis-specific antibodies (n = 10), patients with clinically suspected cases that tested negative for A. cantonensis-an-tibodies (n = 5) and patients with cerebral gnathostomiasis (n = 2) and neurocysticercosis (n = 2) were examined by a single-step polymerase chain reaction (PCR) method using the AC primers for the 66-kDa native protein gene. The PCR method detected A. cantonensis DNA in CSF samples from four of 10 serologically confirmed angiostrongyliasis cases. The PCR results were negative for the remaining CSF samples. The nucleotide sequences of three positive CSF-PCR samples shared 98.8-99.2% similarity with the reference sequence of A. cantonensis. These results indicate the potential application of this PCR assay with clinical CSF samples for additional support in the confirmation of eosinophilic meningitis due to A. cantonensis. |
| first_indexed | 2024-03-12T05:00:26Z |
| format | Article |
| id | doaj.art-05aef7fb5d0043949459036bcd1d93fd |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:00:26Z |
| publishDate | 2013-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-05aef7fb5d0043949459036bcd1d93fd2023-09-03T09:11:46ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602013-02-01108111611810.1590/S0074-02762013000100020S0074-02762013000100020Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patientsPraphathip Eamsobhana0Darawan Wanachiwanawin1Naowarat Dechkum2Anchana Parsartvit3Hoi Sen Yong4Mahidol UniversityMahidol UniversityMahidol UniversityMinistry of Public HealthUniversity of MalayaCerebrospinal fluid (CSF) samples from clinically diagnosed patients with detectable Angiostrongylus canto-nensis-specific antibodies (n = 10), patients with clinically suspected cases that tested negative for A. cantonensis-an-tibodies (n = 5) and patients with cerebral gnathostomiasis (n = 2) and neurocysticercosis (n = 2) were examined by a single-step polymerase chain reaction (PCR) method using the AC primers for the 66-kDa native protein gene. The PCR method detected A. cantonensis DNA in CSF samples from four of 10 serologically confirmed angiostrongyliasis cases. The PCR results were negative for the remaining CSF samples. The nucleotide sequences of three positive CSF-PCR samples shared 98.8-99.2% similarity with the reference sequence of A. cantonensis. These results indicate the potential application of this PCR assay with clinical CSF samples for additional support in the confirmation of eosinophilic meningitis due to A. cantonensis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100020&lng=en&tlng=enAngiostrongylus cantonensiseosinophilic meningitiscerebrospinal fluidPCRDNA sequencing |
| spellingShingle | Praphathip Eamsobhana Darawan Wanachiwanawin Naowarat Dechkum Anchana Parsartvit Hoi Sen Yong Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients Memorias do Instituto Oswaldo Cruz Angiostrongylus cantonensis eosinophilic meningitis cerebrospinal fluid PCR DNA sequencing |
| title | Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients |
| title_full | Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients |
| title_fullStr | Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients |
| title_full_unstemmed | Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients |
| title_short | Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients |
| title_sort | molecular diagnosis of eosinophilic meningitis due to angiostrongylus cantonensis nematoda metastrongyloidea by polymerase chain reaction dna sequencing of cerebrospinal fluids of patients |
| topic | Angiostrongylus cantonensis eosinophilic meningitis cerebrospinal fluid PCR DNA sequencing |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100020&lng=en&tlng=en |
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