<i>Zinc Finger Protein8</i> (<i>GhZFP8</i>) Regulates the Initiation of Trichomes in <i>Arabidopsis</i> and the Development of Fiber in Cotton
Cotton is one of the most important natural fibers used in the textile industry worldwide. It is important to identify the key factors involved in cotton fiber development. In this study, <i>zinc finger protein8</i> (<i>GhZFP8</i>) encoding a C<sub>2</sub>H<sub...
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MDPI AG
2024-02-01
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| Series: | Plants |
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| author | Yongchang Liu Xiaomei Ma Ying Li Xiaoyu Yang Wenhan Cheng |
| author_facet | Yongchang Liu Xiaomei Ma Ying Li Xiaoyu Yang Wenhan Cheng |
| author_sort | Yongchang Liu |
| collection | DOAJ |
| description | Cotton is one of the most important natural fibers used in the textile industry worldwide. It is important to identify the key factors involved in cotton fiber development. In this study, <i>zinc finger protein8</i> (<i>GhZFP8</i>) encoding a C<sub>2</sub>H<sub>2</sub> transcription factor (TF) was cloned from cotton. qPCR showed that the transcripts of <i>GhZFP8</i> in cotton were detected in the leaves and fibers at 3, 6, and 30 days post-anthesis (DPA), but not in the roots, stems, or flowers. The overexpression of <i>GhZFP8</i> increased the trichome number on the siliques, leaves, and inflorescence, but inhibited the growth. The expression of trichome development and cell-elongation-related genes decreased obviously in <i>GhZFP8</i> overexpressor <i>Arabidopsis</i>. Indole-3-acetic acid (IAA) and 1-Aminocyclopropanecarboxylic acid (ACC) contents were much higher in <i>GhZFP8</i> overexpressors than that found in the wild type, but the gibberellin (GA) content was lower. The interference of <i>GhZFP8</i> in cotton caused smaller bolls and shorter fibers than that of the control. The results of DNA affinity purification (DAP)-seq showed that GhZFP8 could bind to the promoter, exon, intron, and intergenic region of the target genes, which are involved in photosynthesis, signal transduction, synthesis of biomass, etc. Our findings implied that GhZFP8 processed multiple biological functions and regulated the development of cotton fiber. |
| first_indexed | 2024-03-07T22:16:31Z |
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| last_indexed | 2024-03-07T22:16:31Z |
| publishDate | 2024-02-01 |
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| spelling | doaj.art-05b1776304934c9ab38efc5bfe743b402024-02-23T15:31:52ZengMDPI AGPlants2223-77472024-02-0113449210.3390/plants13040492<i>Zinc Finger Protein8</i> (<i>GhZFP8</i>) Regulates the Initiation of Trichomes in <i>Arabidopsis</i> and the Development of Fiber in CottonYongchang Liu0Xiaomei Ma1Ying Li2Xiaoyu Yang3Wenhan Cheng4College of Bioengineering, Jingchu University of Technology, Jingmen 448000, ChinaCotton Research Institute, Xinjiang Science Academy of Agriculture and Reclaimation, Shihezi 832000, ChinaCollege of Bioengineering, Jingchu University of Technology, Jingmen 448000, ChinaCollege of Bioengineering, Jingchu University of Technology, Jingmen 448000, ChinaCollege of Bioengineering, Jingchu University of Technology, Jingmen 448000, ChinaCotton is one of the most important natural fibers used in the textile industry worldwide. It is important to identify the key factors involved in cotton fiber development. In this study, <i>zinc finger protein8</i> (<i>GhZFP8</i>) encoding a C<sub>2</sub>H<sub>2</sub> transcription factor (TF) was cloned from cotton. qPCR showed that the transcripts of <i>GhZFP8</i> in cotton were detected in the leaves and fibers at 3, 6, and 30 days post-anthesis (DPA), but not in the roots, stems, or flowers. The overexpression of <i>GhZFP8</i> increased the trichome number on the siliques, leaves, and inflorescence, but inhibited the growth. The expression of trichome development and cell-elongation-related genes decreased obviously in <i>GhZFP8</i> overexpressor <i>Arabidopsis</i>. Indole-3-acetic acid (IAA) and 1-Aminocyclopropanecarboxylic acid (ACC) contents were much higher in <i>GhZFP8</i> overexpressors than that found in the wild type, but the gibberellin (GA) content was lower. The interference of <i>GhZFP8</i> in cotton caused smaller bolls and shorter fibers than that of the control. The results of DNA affinity purification (DAP)-seq showed that GhZFP8 could bind to the promoter, exon, intron, and intergenic region of the target genes, which are involved in photosynthesis, signal transduction, synthesis of biomass, etc. Our findings implied that GhZFP8 processed multiple biological functions and regulated the development of cotton fiber.https://www.mdpi.com/2223-7747/13/4/492<i>Gossypium hirsutum</i>C<sub>2</sub>H<sub>2</sub>zinc finger proteintrichomecotton fiber |
| spellingShingle | Yongchang Liu Xiaomei Ma Ying Li Xiaoyu Yang Wenhan Cheng <i>Zinc Finger Protein8</i> (<i>GhZFP8</i>) Regulates the Initiation of Trichomes in <i>Arabidopsis</i> and the Development of Fiber in Cotton Plants <i>Gossypium hirsutum</i> C<sub>2</sub>H<sub>2</sub> zinc finger protein trichome cotton fiber |
| title | <i>Zinc Finger Protein8</i> (<i>GhZFP8</i>) Regulates the Initiation of Trichomes in <i>Arabidopsis</i> and the Development of Fiber in Cotton |
| title_full | <i>Zinc Finger Protein8</i> (<i>GhZFP8</i>) Regulates the Initiation of Trichomes in <i>Arabidopsis</i> and the Development of Fiber in Cotton |
| title_fullStr | <i>Zinc Finger Protein8</i> (<i>GhZFP8</i>) Regulates the Initiation of Trichomes in <i>Arabidopsis</i> and the Development of Fiber in Cotton |
| title_full_unstemmed | <i>Zinc Finger Protein8</i> (<i>GhZFP8</i>) Regulates the Initiation of Trichomes in <i>Arabidopsis</i> and the Development of Fiber in Cotton |
| title_short | <i>Zinc Finger Protein8</i> (<i>GhZFP8</i>) Regulates the Initiation of Trichomes in <i>Arabidopsis</i> and the Development of Fiber in Cotton |
| title_sort | i zinc finger protein8 i i ghzfp8 i regulates the initiation of trichomes in i arabidopsis i and the development of fiber in cotton |
| topic | <i>Gossypium hirsutum</i> C<sub>2</sub>H<sub>2</sub> zinc finger protein trichome cotton fiber |
| url | https://www.mdpi.com/2223-7747/13/4/492 |
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