Phytochemical Profile and Selective Cytotoxic Activity of a <i>Solanum bulbocastanum</i> Dun. Methanolic Extract on Breast Cancer Cells
<i>Solanum bulbocastanum</i> is a wild potato species, intensively used in potato breeding programs due to its resistance to environmental factors. Thus, its biochemical profile and putative human health-related traits might be transferred into potato cultivars aimed for consumption. Thi...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/23/3262 |
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| author | Mihnea Paraschiv Magda Csiki Zorita Diaconeasa Sonia Socaci Ovidiu Balacescu Elena Rakosy-Tican Daniel Cruceriu |
| author_facet | Mihnea Paraschiv Magda Csiki Zorita Diaconeasa Sonia Socaci Ovidiu Balacescu Elena Rakosy-Tican Daniel Cruceriu |
| author_sort | Mihnea Paraschiv |
| collection | DOAJ |
| description | <i>Solanum bulbocastanum</i> is a wild potato species, intensively used in potato breeding programs due to its resistance to environmental factors. Thus, its biochemical profile and putative human health-related traits might be transferred into potato cultivars aimed for consumption. This study aims to assess the phytochemical profile and the selective cytotoxicity of an <i>S. bulbocastanum</i> extract against breast cancer cells. Dry leaves were subjected to ultrasonication-assisted extraction in methanol [70%]. The phenolic and glycoalkaloid profiles were determined by HPLC-PDA/-ESI+-MS. The volatile profile was investigated by nontargeted ITEX/GC-MS. The extract was tested against three breast cancer cell lines (MCF7, MDA-MB-231, HS578T) and a healthy cell line (HUVEC) by the MTT assay, to assess its selective cytotoxicity. The phenolic profile of the extract revealed high levels of phenolic acids (5959.615 µg/mL extract), and the presence of flavanols (818.919 µg/mL extract). The diversity of the volatile compounds was rather low (nine compounds), whereas no glycoalkaloids were identified, only two alkaloid precursors (813.524 µg/mL extract). The extract proved to be cytotoxic towards all breast cancer cell lines (IC<sub>50</sub> values between 139.1 and 356,1 µg/mL), with selectivity coefficients between 1.96 and 4.96 when compared with its toxicity on HUVECs. Based on these results we conclude that the exerted cytotoxic activity of the extract is due to its high polyphenolic content, whereas the lack of <i>Solanaceae</i>-specific glycoalkaloids might be responsible for its high selectivity against breast cancer cells in comparison with other extract obtained from wild <i>Solanum</i> species. However, further research is needed in order to assess the cytotoxicity of the individual compounds found in the extract, as well as the anti-tumor potential of the <i>S. bulbocastanum</i> tubers. |
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| language | English |
| last_indexed | 2024-03-09T17:35:24Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-05b4393d593c4faeaf3f83f433b02d922023-11-24T11:55:01ZengMDPI AGPlants2223-77472022-11-011123326210.3390/plants11233262Phytochemical Profile and Selective Cytotoxic Activity of a <i>Solanum bulbocastanum</i> Dun. Methanolic Extract on Breast Cancer CellsMihnea Paraschiv0Magda Csiki1Zorita Diaconeasa2Sonia Socaci3Ovidiu Balacescu4Elena Rakosy-Tican5Daniel Cruceriu6Department of Molecular Biology and Biotechnology, “Babes-Bolyai” University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, “Babes-Bolyai” University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, RomaniaFaculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăştur, 400372 Cluj-Napoca, RomaniaFaculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăştur, 400372 Cluj-Napoca, RomaniaDepartment of Genetics, Genomics and Experimental Pathology, The Oncology Institute “Prof. Dr. Ion Chiricuta”, 34-36 Republicii Street, 400015 Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, “Babes-Bolyai” University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, “Babes-Bolyai” University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania<i>Solanum bulbocastanum</i> is a wild potato species, intensively used in potato breeding programs due to its resistance to environmental factors. Thus, its biochemical profile and putative human health-related traits might be transferred into potato cultivars aimed for consumption. This study aims to assess the phytochemical profile and the selective cytotoxicity of an <i>S. bulbocastanum</i> extract against breast cancer cells. Dry leaves were subjected to ultrasonication-assisted extraction in methanol [70%]. The phenolic and glycoalkaloid profiles were determined by HPLC-PDA/-ESI+-MS. The volatile profile was investigated by nontargeted ITEX/GC-MS. The extract was tested against three breast cancer cell lines (MCF7, MDA-MB-231, HS578T) and a healthy cell line (HUVEC) by the MTT assay, to assess its selective cytotoxicity. The phenolic profile of the extract revealed high levels of phenolic acids (5959.615 µg/mL extract), and the presence of flavanols (818.919 µg/mL extract). The diversity of the volatile compounds was rather low (nine compounds), whereas no glycoalkaloids were identified, only two alkaloid precursors (813.524 µg/mL extract). The extract proved to be cytotoxic towards all breast cancer cell lines (IC<sub>50</sub> values between 139.1 and 356,1 µg/mL), with selectivity coefficients between 1.96 and 4.96 when compared with its toxicity on HUVECs. Based on these results we conclude that the exerted cytotoxic activity of the extract is due to its high polyphenolic content, whereas the lack of <i>Solanaceae</i>-specific glycoalkaloids might be responsible for its high selectivity against breast cancer cells in comparison with other extract obtained from wild <i>Solanum</i> species. However, further research is needed in order to assess the cytotoxicity of the individual compounds found in the extract, as well as the anti-tumor potential of the <i>S. bulbocastanum</i> tubers.https://www.mdpi.com/2223-7747/11/23/3262<i>Solanaceae</i>phenolic acidsalkaloidsvolatile compoundsanticancer activityselective cytotoxicity |
| spellingShingle | Mihnea Paraschiv Magda Csiki Zorita Diaconeasa Sonia Socaci Ovidiu Balacescu Elena Rakosy-Tican Daniel Cruceriu Phytochemical Profile and Selective Cytotoxic Activity of a <i>Solanum bulbocastanum</i> Dun. Methanolic Extract on Breast Cancer Cells Plants <i>Solanaceae</i> phenolic acids alkaloids volatile compounds anticancer activity selective cytotoxicity |
| title | Phytochemical Profile and Selective Cytotoxic Activity of a <i>Solanum bulbocastanum</i> Dun. Methanolic Extract on Breast Cancer Cells |
| title_full | Phytochemical Profile and Selective Cytotoxic Activity of a <i>Solanum bulbocastanum</i> Dun. Methanolic Extract on Breast Cancer Cells |
| title_fullStr | Phytochemical Profile and Selective Cytotoxic Activity of a <i>Solanum bulbocastanum</i> Dun. Methanolic Extract on Breast Cancer Cells |
| title_full_unstemmed | Phytochemical Profile and Selective Cytotoxic Activity of a <i>Solanum bulbocastanum</i> Dun. Methanolic Extract on Breast Cancer Cells |
| title_short | Phytochemical Profile and Selective Cytotoxic Activity of a <i>Solanum bulbocastanum</i> Dun. Methanolic Extract on Breast Cancer Cells |
| title_sort | phytochemical profile and selective cytotoxic activity of a i solanum bulbocastanum i dun methanolic extract on breast cancer cells |
| topic | <i>Solanaceae</i> phenolic acids alkaloids volatile compounds anticancer activity selective cytotoxicity |
| url | https://www.mdpi.com/2223-7747/11/23/3262 |
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