Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4)
Relatively few studies have been focused so far on magnesium–isotope fractionation during plant growth, element uptake from soil, root-to-leaves transport and during chlorophylls biosynthesis. In this work, maize and garden cress were hydroponically grown in identical conditions in order to examine...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-04-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/25/7/1644 |
_version_ | 1797571534521368576 |
---|---|
author | Katarzyna Wrobel Jakub Karasiński Andrii Tupys Missael Antonio Arroyo Negrete Ludwik Halicz Kazimierz Wrobel Ewa Bulska |
author_facet | Katarzyna Wrobel Jakub Karasiński Andrii Tupys Missael Antonio Arroyo Negrete Ludwik Halicz Kazimierz Wrobel Ewa Bulska |
author_sort | Katarzyna Wrobel |
collection | DOAJ |
description | Relatively few studies have been focused so far on magnesium–isotope fractionation during plant growth, element uptake from soil, root-to-leaves transport and during chlorophylls biosynthesis. In this work, maize and garden cress were hydroponically grown in identical conditions in order to examine if the carbon fixation pathway (C4, C3, respectively) might have impact on Mg-isotope fractionation in chlorophyll-a. The pigment was purified from plants extracts by preparative reversed phase chromatography, and its identity was confirmed by high-resolution mass spectrometry. The green parts of plants and chlorophyll-a fractions were acid-digested and submitted to ion chromatography coupled through desolvation system to multiple collector inductively coupled plasma-mass spectrometry. Clear preference for heavy Mg-isotopes was found in maize green parts (∆<sup>26</sup>Mg<sub>plant-nutrient</sub> 0.65, 0.74 for two biological replicates, respectively) and in chlorophyll-a (∆<sup>26</sup>Mg<sub>chlorophyll-plant</sub> 1.51, 2.19). In garden cress, heavy isotopes were depleted in green parts (∆<sup>26</sup>Mg<sub>plant-nutrient</sub> (−0.87)–(−0.92)) and the preference for heavy isotopes in chlorophyll-a was less marked relative to maize (∆<sup>26</sup>Mg<sub>chlorophyll-plant</sub> 0.55–0.52). The observed effect might be ascribed to overall higher production of energy in form of adenosine triphosphate (ATP), required for carbon fixation in C4 compared to C3, which could reduce kinetic barrier and make equilibrium fractionation prevailing during magnesium incorporation to protoporphyrin ring. |
first_indexed | 2024-03-10T20:41:34Z |
format | Article |
id | doaj.art-05b60a5cda0f40b48e1bdf087b67f4db |
institution | Directory Open Access Journal |
issn | 1420-3049 |
language | English |
last_indexed | 2024-03-10T20:41:34Z |
publishDate | 2020-04-01 |
publisher | MDPI AG |
record_format | Article |
series | Molecules |
spelling | doaj.art-05b60a5cda0f40b48e1bdf087b67f4db2023-11-19T20:35:11ZengMDPI AGMolecules1420-30492020-04-01257164410.3390/molecules25071644Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4)Katarzyna Wrobel0Jakub Karasiński1Andrii Tupys2Missael Antonio Arroyo Negrete3Ludwik Halicz4Kazimierz Wrobel5Ewa Bulska6Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-093 Warszawa, PolandFaculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-093 Warszawa, PolandFaculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-093 Warszawa, PolandChemistry Department, University of Guanajuato, L. de Retana 5, 36000 Guanajuato, MexicoFaculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-093 Warszawa, PolandChemistry Department, University of Guanajuato, L. de Retana 5, 36000 Guanajuato, MexicoFaculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-093 Warszawa, PolandRelatively few studies have been focused so far on magnesium–isotope fractionation during plant growth, element uptake from soil, root-to-leaves transport and during chlorophylls biosynthesis. In this work, maize and garden cress were hydroponically grown in identical conditions in order to examine if the carbon fixation pathway (C4, C3, respectively) might have impact on Mg-isotope fractionation in chlorophyll-a. The pigment was purified from plants extracts by preparative reversed phase chromatography, and its identity was confirmed by high-resolution mass spectrometry. The green parts of plants and chlorophyll-a fractions were acid-digested and submitted to ion chromatography coupled through desolvation system to multiple collector inductively coupled plasma-mass spectrometry. Clear preference for heavy Mg-isotopes was found in maize green parts (∆<sup>26</sup>Mg<sub>plant-nutrient</sub> 0.65, 0.74 for two biological replicates, respectively) and in chlorophyll-a (∆<sup>26</sup>Mg<sub>chlorophyll-plant</sub> 1.51, 2.19). In garden cress, heavy isotopes were depleted in green parts (∆<sup>26</sup>Mg<sub>plant-nutrient</sub> (−0.87)–(−0.92)) and the preference for heavy isotopes in chlorophyll-a was less marked relative to maize (∆<sup>26</sup>Mg<sub>chlorophyll-plant</sub> 0.55–0.52). The observed effect might be ascribed to overall higher production of energy in form of adenosine triphosphate (ATP), required for carbon fixation in C4 compared to C3, which could reduce kinetic barrier and make equilibrium fractionation prevailing during magnesium incorporation to protoporphyrin ring.https://www.mdpi.com/1420-3049/25/7/1644Mg-isotope fractionationchlorophyllC3 plantsC4 plantsion chromatographymultiple collector-inductively coupled mass spectrometry |
spellingShingle | Katarzyna Wrobel Jakub Karasiński Andrii Tupys Missael Antonio Arroyo Negrete Ludwik Halicz Kazimierz Wrobel Ewa Bulska Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4) Molecules Mg-isotope fractionation chlorophyll C3 plants C4 plants ion chromatography multiple collector-inductively coupled mass spectrometry |
title | Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4) |
title_full | Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4) |
title_fullStr | Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4) |
title_full_unstemmed | Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4) |
title_short | Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4) |
title_sort | magnesium isotope fractionation in chlorophyll a extracted from two plants with different pathways of carbon fixation c3 c4 |
topic | Mg-isotope fractionation chlorophyll C3 plants C4 plants ion chromatography multiple collector-inductively coupled mass spectrometry |
url | https://www.mdpi.com/1420-3049/25/7/1644 |
work_keys_str_mv | AT katarzynawrobel magnesiumisotopefractionationinchlorophyllaextractedfromtwoplantswithdifferentpathwaysofcarbonfixationc3c4 AT jakubkarasinski magnesiumisotopefractionationinchlorophyllaextractedfromtwoplantswithdifferentpathwaysofcarbonfixationc3c4 AT andriitupys magnesiumisotopefractionationinchlorophyllaextractedfromtwoplantswithdifferentpathwaysofcarbonfixationc3c4 AT missaelantonioarroyonegrete magnesiumisotopefractionationinchlorophyllaextractedfromtwoplantswithdifferentpathwaysofcarbonfixationc3c4 AT ludwikhalicz magnesiumisotopefractionationinchlorophyllaextractedfromtwoplantswithdifferentpathwaysofcarbonfixationc3c4 AT kazimierzwrobel magnesiumisotopefractionationinchlorophyllaextractedfromtwoplantswithdifferentpathwaysofcarbonfixationc3c4 AT ewabulska magnesiumisotopefractionationinchlorophyllaextractedfromtwoplantswithdifferentpathwaysofcarbonfixationc3c4 |