Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode array
Summary: Here, we present a protocol for the fabrication of transparent implantable electrode arrays for integrating optogenetics and electrophysiology. We describe steps for fabricating microelectrodes using the conductive polymer poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate). We then det...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724000741 |
| _version_ | 1797287541196455936 |
|---|---|
| author | Young Uk Cho Ju Young Lee Ki Jun Yu |
| author_facet | Young Uk Cho Ju Young Lee Ki Jun Yu |
| author_sort | Young Uk Cho |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for the fabrication of transparent implantable electrode arrays for integrating optogenetics and electrophysiology. We describe steps for fabricating microelectrodes using the conductive polymer poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate). We then detail procedures for analyzing performance of the electrodes and recording light-evoked neural activities from the transgenic mouse. This protocol utilizes photolithography rather than conventional electrodeposition.For complete details on the use and execution of this protocol, please refer to Cho et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-07T18:34:39Z |
| format | Article |
| id | doaj.art-05b6478ed46541f9bffdfa6888987b56 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-07T18:34:39Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-05b6478ed46541f9bffdfa6888987b562024-03-02T04:54:57ZengElsevierSTAR Protocols2666-16672024-03-0151102909Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode arrayYoung Uk Cho0Ju Young Lee1Ki Jun Yu2School of Electrical and Electronic Engineering, Yonsei University, 50 Yonsei-ro, Seodaemungu, Seoul 03722, Republic of KoreaSchool of Electrical and Electronic Engineering, Yonsei University, 50 Yonsei-ro, Seodaemungu, Seoul 03722, Republic of KoreaSchool of Electrical and Electronic Engineering, Yonsei University, 50 Yonsei-ro, Seodaemungu, Seoul 03722, Republic of Korea; School of Electrical and Electronic Engineering, YU-KIST Institute, Yonsei University, 50 Yonsei-ro, Seodaemungu, Seoul 03722, Republic of Korea; Corresponding authorSummary: Here, we present a protocol for the fabrication of transparent implantable electrode arrays for integrating optogenetics and electrophysiology. We describe steps for fabricating microelectrodes using the conductive polymer poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate). We then detail procedures for analyzing performance of the electrodes and recording light-evoked neural activities from the transgenic mouse. This protocol utilizes photolithography rather than conventional electrodeposition.For complete details on the use and execution of this protocol, please refer to Cho et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724000741NeuroscienceBiotechnology and bioengineering |
| spellingShingle | Young Uk Cho Ju Young Lee Ki Jun Yu Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode array STAR Protocols Neuroscience Biotechnology and bioengineering |
| title | Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode array |
| title_full | Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode array |
| title_fullStr | Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode array |
| title_full_unstemmed | Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode array |
| title_short | Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode array |
| title_sort | integration of in vivo electrophysiology and optogenetics in rodents with pedot pss neural electrode array |
| topic | Neuroscience Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724000741 |
| work_keys_str_mv | AT youngukcho integrationofinvivoelectrophysiologyandoptogeneticsinrodentswithpedotpssneuralelectrodearray AT juyounglee integrationofinvivoelectrophysiologyandoptogeneticsinrodentswithpedotpssneuralelectrodearray AT kijunyu integrationofinvivoelectrophysiologyandoptogeneticsinrodentswithpedotpssneuralelectrodearray |