Biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae
Biological activity of a plant extract (common rue, Ruta chalepensis) and a semi purifíed fraction (from "tacaco cimarrón", Sechium pittieri) on mahogany shootborer larvas (Hypsipyla grandella) was studied. A randomized complete block design, with four replications, was used. H. grandella...
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| Language: | English |
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Vicerractoría Investigación
2001-06-01
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| Series: | Revista de Biología Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442001000200007 |
| _version_ | 1797714675103694848 |
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| author | Fernando Mancebo Luko Hilje Gerardo A. Mora Víctor H. Castro Rodolfo Salazar |
| author_facet | Fernando Mancebo Luko Hilje Gerardo A. Mora Víctor H. Castro Rodolfo Salazar |
| author_sort | Fernando Mancebo |
| collection | DOAJ |
| description | Biological activity of a plant extract (common rue, Ruta chalepensis) and a semi purifíed fraction (from "tacaco cimarrón", Sechium pittieri) on mahogany shootborer larvas (Hypsipyla grandella) was studied. A randomized complete block design, with four replications, was used. H. grandella third instar larvas were exposed for 24 h to Cedrela odorata leaf discs dipped in several treatment dissolutions of each extract (0.1, 0.32, 1.0, 3.20, and 10%); afterwards, each larva was transferred to a flask containing an artificial diet and was allowed to complete its development. Variables measured included food consumption (foliar area eaten in 24 h), mortality, and developmental effects (developmental time for each larval instar and the pupa, and pupal weight). The common rue extract showed a clear antifeedant activity at a concentration as low as 0.32%, whereas the "tacaco cimarrón" fraction caused toxicity, especially at the two highest concentrations (3.20 and 10%).<br>Se estudió la actividad biológica de un extracto de follaje de ruda (Ruta chalepensis) y de una fracción semipurificada de "tacaco cimarrón" (Sechium pittieri) sobre las larvas del gusano barrenador de las meliáceas (Hypsipyla grandella). Se utilizó un diseño de bloques completos al azar, con cuatro repeticiones. Durante 24 h se expusieron larvas de tercer estadio de H. grandella a discos de follaje de Cedrela odorata impregnados con cada tratamiento. Estos consistieron en disoluciones de cada extracto (0.1, 0.32, 1.0, 3.20 y 10%); posteriormente cada larva se transfirió a un frasco que contenía dieta artificial, donde se le permitió completar su desarrollo. Las variables de respuesta fueron el consumo de alimento (área foliar comida en 24 h), la mortalidad y efectos sobre el desarrollo (tiempo de desarrollo de cada estadio larval y de la pupa, y el peso de la pupa). El extracto de ruda causó fagodisuasión a una concentración de apenas 0.32%, mientras que la fracción de "tacaco cimarrón" provocó toxicidad especialmente a las dos mayores concentraciones (3.20 y 10%). |
| first_indexed | 2024-03-12T07:55:27Z |
| format | Article |
| id | doaj.art-05b94517e3794e34a669fe2e5250947c |
| institution | Directory Open Access Journal |
| issn | 0034-7744 2215-2075 |
| language | English |
| last_indexed | 2024-03-12T07:55:27Z |
| publishDate | 2001-06-01 |
| publisher | Vicerractoría Investigación |
| record_format | Article |
| series | Revista de Biología Tropical |
| spelling | doaj.art-05b94517e3794e34a669fe2e5250947c2023-09-02T20:11:58ZengVicerractoría InvestigaciónRevista de Biología Tropical0034-77442215-20752001-06-01492501508Biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvaeFernando ManceboLuko HiljeGerardo A. MoraVíctor H. CastroRodolfo SalazarBiological activity of a plant extract (common rue, Ruta chalepensis) and a semi purifíed fraction (from "tacaco cimarrón", Sechium pittieri) on mahogany shootborer larvas (Hypsipyla grandella) was studied. A randomized complete block design, with four replications, was used. H. grandella third instar larvas were exposed for 24 h to Cedrela odorata leaf discs dipped in several treatment dissolutions of each extract (0.1, 0.32, 1.0, 3.20, and 10%); afterwards, each larva was transferred to a flask containing an artificial diet and was allowed to complete its development. Variables measured included food consumption (foliar area eaten in 24 h), mortality, and developmental effects (developmental time for each larval instar and the pupa, and pupal weight). The common rue extract showed a clear antifeedant activity at a concentration as low as 0.32%, whereas the "tacaco cimarrón" fraction caused toxicity, especially at the two highest concentrations (3.20 and 10%).<br>Se estudió la actividad biológica de un extracto de follaje de ruda (Ruta chalepensis) y de una fracción semipurificada de "tacaco cimarrón" (Sechium pittieri) sobre las larvas del gusano barrenador de las meliáceas (Hypsipyla grandella). Se utilizó un diseño de bloques completos al azar, con cuatro repeticiones. Durante 24 h se expusieron larvas de tercer estadio de H. grandella a discos de follaje de Cedrela odorata impregnados con cada tratamiento. Estos consistieron en disoluciones de cada extracto (0.1, 0.32, 1.0, 3.20 y 10%); posteriormente cada larva se transfirió a un frasco que contenía dieta artificial, donde se le permitió completar su desarrollo. Las variables de respuesta fueron el consumo de alimento (área foliar comida en 24 h), la mortalidad y efectos sobre el desarrollo (tiempo de desarrollo de cada estadio larval y de la pupa, y el peso de la pupa). El extracto de ruda causó fagodisuasión a una concentración de apenas 0.32%, mientras que la fracción de "tacaco cimarrón" provocó toxicidad especialmente a las dos mayores concentraciones (3.20 y 10%).http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442001000200007Hypsipyla grandella"tacaco cimarrón"mahogany shootborerMeliaceaeplant extractscommon rueantifeedantmortality |
| spellingShingle | Fernando Mancebo Luko Hilje Gerardo A. Mora Víctor H. Castro Rodolfo Salazar Biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae Revista de Biología Tropical Hypsipyla grandella "tacaco cimarrón" mahogany shootborer Meliaceae plant extracts common rue antifeedant mortality |
| title | Biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae |
| title_full | Biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae |
| title_fullStr | Biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae |
| title_full_unstemmed | Biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae |
| title_short | Biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae |
| title_sort | biological activity of ruta chalepensis rutaceae and sechium pittieri cucurbitaceae extracts on hypsipyla grandella lepidoptera pyralidae larvae |
| topic | Hypsipyla grandella "tacaco cimarrón" mahogany shootborer Meliaceae plant extracts common rue antifeedant mortality |
| url | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442001000200007 |
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