Identification of Novel Cetacean Poxviruses in Cetaceans Stranded in South West England.
Poxvirus infections in marine mammals have been mainly reported through their clinical lesions and electron microscopy (EM). Poxvirus particles in association with such lesions have been demonstrated by EM and were previously classified as two new viruses, cetacean poxvirus 1 (CePV-1) and cetacean p...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4457422?pdf=render |
| _version_ | 1818435182117519360 |
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| author | James Barnett Akbar Dastjerdi Nick Davison Rob Deaville David Everest Julie Peake Christopher Finnegan Paul Jepson Falko Steinbach |
| author_facet | James Barnett Akbar Dastjerdi Nick Davison Rob Deaville David Everest Julie Peake Christopher Finnegan Paul Jepson Falko Steinbach |
| author_sort | James Barnett |
| collection | DOAJ |
| description | Poxvirus infections in marine mammals have been mainly reported through their clinical lesions and electron microscopy (EM). Poxvirus particles in association with such lesions have been demonstrated by EM and were previously classified as two new viruses, cetacean poxvirus 1 (CePV-1) and cetacean poxvirus 2 (CePV-2). In this study, epidermal pox lesions in cetaceans stranded in South West England (Cornwall) between 2008 and 2012 were investigated by electron microscopy and molecular analysis. PCR and sequencing of a highly conserved region within the viral DNA polymerase gene ruled out both parapox- and orthopoxviruses. Moreover, phylogenetic analysis of the PCR product clustered the sequences with those previously described as cetacean poxviruses. However, taking the close genetic distance of this gene fragment across the family of poxviridae into account, it is reasonable to postulate further, novel cetacean poxvirus species. The nucleotide similarity within each cluster (tentative species) detected ranged from 98.6% to 100%, whilst the similarity between the clusters was no more than 95%. The detection of several species of poxvirus in different cetacean species confirms the likelihood of a heterogeneous cetacean poxvirus genus, comparable to the heterogeneity observed in other poxvirus genera. |
| first_indexed | 2024-12-14T16:48:49Z |
| format | Article |
| id | doaj.art-05b9a3a5cf20444896c504defab47442 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T16:48:49Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-05b9a3a5cf20444896c504defab474422022-12-21T22:54:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012431510.1371/journal.pone.0124315Identification of Novel Cetacean Poxviruses in Cetaceans Stranded in South West England.James BarnettAkbar DastjerdiNick DavisonRob DeavilleDavid EverestJulie PeakeChristopher FinneganPaul JepsonFalko SteinbachPoxvirus infections in marine mammals have been mainly reported through their clinical lesions and electron microscopy (EM). Poxvirus particles in association with such lesions have been demonstrated by EM and were previously classified as two new viruses, cetacean poxvirus 1 (CePV-1) and cetacean poxvirus 2 (CePV-2). In this study, epidermal pox lesions in cetaceans stranded in South West England (Cornwall) between 2008 and 2012 were investigated by electron microscopy and molecular analysis. PCR and sequencing of a highly conserved region within the viral DNA polymerase gene ruled out both parapox- and orthopoxviruses. Moreover, phylogenetic analysis of the PCR product clustered the sequences with those previously described as cetacean poxviruses. However, taking the close genetic distance of this gene fragment across the family of poxviridae into account, it is reasonable to postulate further, novel cetacean poxvirus species. The nucleotide similarity within each cluster (tentative species) detected ranged from 98.6% to 100%, whilst the similarity between the clusters was no more than 95%. The detection of several species of poxvirus in different cetacean species confirms the likelihood of a heterogeneous cetacean poxvirus genus, comparable to the heterogeneity observed in other poxvirus genera.http://europepmc.org/articles/PMC4457422?pdf=render |
| spellingShingle | James Barnett Akbar Dastjerdi Nick Davison Rob Deaville David Everest Julie Peake Christopher Finnegan Paul Jepson Falko Steinbach Identification of Novel Cetacean Poxviruses in Cetaceans Stranded in South West England. PLoS ONE |
| title | Identification of Novel Cetacean Poxviruses in Cetaceans Stranded in South West England. |
| title_full | Identification of Novel Cetacean Poxviruses in Cetaceans Stranded in South West England. |
| title_fullStr | Identification of Novel Cetacean Poxviruses in Cetaceans Stranded in South West England. |
| title_full_unstemmed | Identification of Novel Cetacean Poxviruses in Cetaceans Stranded in South West England. |
| title_short | Identification of Novel Cetacean Poxviruses in Cetaceans Stranded in South West England. |
| title_sort | identification of novel cetacean poxviruses in cetaceans stranded in south west england |
| url | http://europepmc.org/articles/PMC4457422?pdf=render |
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