Characterization of the 1<sup>st </sup>and 2<sup>nd </sup>EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism

Characterization of the 1<sup>st </sup>and 2<sup>nd </sup>EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism

<p>Abstract</p> <p>Background</p> <p>Superoxide generated by non-phagocytic NADPH oxidases (NOXs) is of growing importance for physiology and pathobiology. The calcium binding domain (CaBD) of NOX5 contains four EF-hands, each binding one calcium ion. To better understa...

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Bibliographic Details
Main Authors: Wei Chin-Chuan, Reynolds Nicole, Palka Christina, Wetherell Kristen, Boyle Tiffany, Yang Ya-Ping, Wang Zhi-Qiang, Stuehr Dennis J
Format: Article
Language:English
Published: BMC 2012-04-01
Series:Chemistry Central Journal
Subjects:
EF-hand
Calcium binding
NADPH Oxidase 5
Fluorescence
Isothermal titration calorimetry
Online Access:http://journal.chemistrycentral.com/content/6/1/29

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