Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma
The total vitamin C content in human plasma is widely accepted as an indicator of the tissue status of vitamin C. A liquid chromatography method with ultraviolet detector (264 nm) for measuring ascorbic acid in human plasma was developed. A C18 reversed-phase column and cetrimide as an ion-pairing a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2001-09-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/2427 |
| _version_ | 1828371976877506560 |
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| author | Oveisi MR Sadeghi N |
| author_facet | Oveisi MR Sadeghi N |
| author_sort | Oveisi MR |
| collection | DOAJ |
| description | The total vitamin C content in human plasma is widely accepted as an indicator of the tissue status of vitamin C. A liquid chromatography method with ultraviolet detector (264 nm) for measuring ascorbic acid in human plasma was developed. A C18 reversed-phase column and cetrimide as an ion-pairing agent was employed. Ascorbic acid (AA) was measured after reducing L-dehydroascorbic acid to L-ascorbic acid with dithiothreitol. The stability of the ascorbic acid in plasma, metaphosphoric acid and trichloroacetic acid was also evaluated. The analytical parameters, including linearity (1-60 µg/ml), accuracy (98.98%), repeatability (2.8%) and reproducibility (7.2%), showed that the method is reliable for measuring the total vitamin C content in plasma. |
| first_indexed | 2024-04-14T06:56:43Z |
| format | Article |
| id | doaj.art-05bba042df7e4adba3ae80d23ad3d695 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-04-14T06:56:43Z |
| publishDate | 2001-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-05bba042df7e4adba3ae80d23ad3d6952022-12-22T02:06:51ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942001-09-01393Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma Oveisi MR0 Sadeghi N1 The total vitamin C content in human plasma is widely accepted as an indicator of the tissue status of vitamin C. A liquid chromatography method with ultraviolet detector (264 nm) for measuring ascorbic acid in human plasma was developed. A C18 reversed-phase column and cetrimide as an ion-pairing agent was employed. Ascorbic acid (AA) was measured after reducing L-dehydroascorbic acid to L-ascorbic acid with dithiothreitol. The stability of the ascorbic acid in plasma, metaphosphoric acid and trichloroacetic acid was also evaluated. The analytical parameters, including linearity (1-60 µg/ml), accuracy (98.98%), repeatability (2.8%) and reproducibility (7.2%), showed that the method is reliable for measuring the total vitamin C content in plasma.https://acta.tums.ac.ir/index.php/acta/article/view/2427L-Ascorbic acidTotal vitamin C |
| spellingShingle | Oveisi MR Sadeghi N Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma Acta Medica Iranica L-Ascorbic acid Total vitamin C |
| title | Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma |
| title_full | Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma |
| title_fullStr | Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma |
| title_full_unstemmed | Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma |
| title_short | Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma |
| title_sort | determination of total l ascorbic acid by high performance liquid chromatography in human plasma |
| topic | L-Ascorbic acid Total vitamin C |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/2427 |
| work_keys_str_mv | AT oveisimr determinationoftotallascorbicacidbyhighperformanceliquidchromatographyinhumanplasma AT sadeghin determinationoftotallascorbicacidbyhighperformanceliquidchromatographyinhumanplasma |