Fetoscopic laser coagulation of intertwin anastomoses reduces discordant placental autophagic activities in discordant twin growth
Objectives: To investigate placental autophagic activity in cases of twin-twin transfusion syndrome (TTTS) after successful laser therapy and to evaluate the effect of intertwin anastomoses on discordant placenta autophagic activity in monochorionic twins with one twin exhibiting selective intrauter...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-10-01
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| Series: | Taiwanese Journal of Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1028455915001692 |
| _version_ | 1819096534491332608 |
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| author | Yao-Lung Chang Tzu-Hao Wang Shuenn-Dyh Chang An-Shine Chao Peter C.C. Hsieh |
| author_facet | Yao-Lung Chang Tzu-Hao Wang Shuenn-Dyh Chang An-Shine Chao Peter C.C. Hsieh |
| author_sort | Yao-Lung Chang |
| collection | DOAJ |
| description | Objectives: To investigate placental autophagic activity in cases of twin-twin transfusion syndrome (TTTS) after successful laser therapy and to evaluate the effect of intertwin anastomoses on discordant placenta autophagic activity in monochorionic twins with one twin exhibiting selective intrauterine growth restriction.
Materials and methods: Placenta samples were prospectively obtained from 11 cases of successful TTTS post-laser therapy with two living babies. Among these infants, five infants had selective intrauterine growth restriction (sIUGR), based on the definition of a birth weight below the 10th percentile. After protein extraction, western blot tests were used to determine the amount of placenta microtubule-associated protein 1A/1B-light chain 3 (LC3)-II protein in the two individual placenta territories of the twin pair. The LC3-II protein fold change ratio (FCR) in a twin pair was defined as the LC3-II protein fold value over β-actin of the smaller twin divided by the LC3-II protein fold value over β-actin of the larger twin.
Results: The LC3-II FCRs were not significantly different between TTTS with sIUGR and TTTS without sIUGR, after successful laser therapy.
Conclusion: The discordance of placenta autophagic activity in the monochorionic twin with sIUGR was reduced after laser coagulation of the intertwin anastomoses, which may result from the effect of correction of the discordant intertwin placenta perfusion. |
| first_indexed | 2024-12-22T00:00:43Z |
| format | Article |
| id | doaj.art-05c0ae5abb864f65bb97ce02159a76ea |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-12-22T00:00:43Z |
| publishDate | 2015-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-05c0ae5abb864f65bb97ce02159a76ea2022-12-21T18:45:41ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592015-10-0154558058210.1016/j.tjog.2015.08.011Fetoscopic laser coagulation of intertwin anastomoses reduces discordant placental autophagic activities in discordant twin growthYao-Lung ChangTzu-Hao WangShuenn-Dyh ChangAn-Shine ChaoPeter C.C. HsiehObjectives: To investigate placental autophagic activity in cases of twin-twin transfusion syndrome (TTTS) after successful laser therapy and to evaluate the effect of intertwin anastomoses on discordant placenta autophagic activity in monochorionic twins with one twin exhibiting selective intrauterine growth restriction. Materials and methods: Placenta samples were prospectively obtained from 11 cases of successful TTTS post-laser therapy with two living babies. Among these infants, five infants had selective intrauterine growth restriction (sIUGR), based on the definition of a birth weight below the 10th percentile. After protein extraction, western blot tests were used to determine the amount of placenta microtubule-associated protein 1A/1B-light chain 3 (LC3)-II protein in the two individual placenta territories of the twin pair. The LC3-II protein fold change ratio (FCR) in a twin pair was defined as the LC3-II protein fold value over β-actin of the smaller twin divided by the LC3-II protein fold value over β-actin of the larger twin. Results: The LC3-II FCRs were not significantly different between TTTS with sIUGR and TTTS without sIUGR, after successful laser therapy. Conclusion: The discordance of placenta autophagic activity in the monochorionic twin with sIUGR was reduced after laser coagulation of the intertwin anastomoses, which may result from the effect of correction of the discordant intertwin placenta perfusion.http://www.sciencedirect.com/science/article/pii/S1028455915001692autophagyLC3monochorionic twinplacentatwin-twin transfusion syndrome |
| spellingShingle | Yao-Lung Chang Tzu-Hao Wang Shuenn-Dyh Chang An-Shine Chao Peter C.C. Hsieh Fetoscopic laser coagulation of intertwin anastomoses reduces discordant placental autophagic activities in discordant twin growth Taiwanese Journal of Obstetrics & Gynecology autophagy LC3 monochorionic twin placenta twin-twin transfusion syndrome |
| title | Fetoscopic laser coagulation of intertwin anastomoses reduces discordant placental autophagic activities in discordant twin growth |
| title_full | Fetoscopic laser coagulation of intertwin anastomoses reduces discordant placental autophagic activities in discordant twin growth |
| title_fullStr | Fetoscopic laser coagulation of intertwin anastomoses reduces discordant placental autophagic activities in discordant twin growth |
| title_full_unstemmed | Fetoscopic laser coagulation of intertwin anastomoses reduces discordant placental autophagic activities in discordant twin growth |
| title_short | Fetoscopic laser coagulation of intertwin anastomoses reduces discordant placental autophagic activities in discordant twin growth |
| title_sort | fetoscopic laser coagulation of intertwin anastomoses reduces discordant placental autophagic activities in discordant twin growth |
| topic | autophagy LC3 monochorionic twin placenta twin-twin transfusion syndrome |
| url | http://www.sciencedirect.com/science/article/pii/S1028455915001692 |
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