<i>Piriformospora indica</i> Increases Resistance to <i>Fusarium pseudograminearum</i> in Wheat by Inducing Phenylpropanoid Pathway
Fusarium crown rot (FCR), mainly caused by <i>Fusarium pseudograminearum</i>, not only seriously threatens the yield and quality of wheat, but also endangers the health and safety of humans and livestock. <i>Piriformospora indica</i> is a root endophytic fungus that colonizes...
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8797 |
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| author | Liang Li Ruiying Hao Xiurong Yang Yu Feng Zhenghui Bi |
| author_facet | Liang Li Ruiying Hao Xiurong Yang Yu Feng Zhenghui Bi |
| author_sort | Liang Li |
| collection | DOAJ |
| description | Fusarium crown rot (FCR), mainly caused by <i>Fusarium pseudograminearum</i>, not only seriously threatens the yield and quality of wheat, but also endangers the health and safety of humans and livestock. <i>Piriformospora indica</i> is a root endophytic fungus that colonizes plant roots extensively and can effectively promote plant growth and improve plant resistance to biotic and abiotic stresses. In this study, the mechanism of FCR resistance mediated by <i>P. indica</i> in wheat was revealed from the phenylpropanoid metabolic pathway. The results showed that the colonization of <i>P. indica</i> significantly reduced the progression of wheat disease, the amount of <i>F. pseudograminearum</i> colonization, and the content of deoxynivalenol (DON) in wheat roots. RNA-seq suggested that <i>P. indica</i> colonization could reduce the number of differentially expressed genes (DEGs) in the transcriptome caused by <i>F. pseudograminearum</i> infection. The DEGs induced by the colonization of <i>P. indica</i> were partially enriched in phenylpropanoid biosynthesis. Transcriptome sequencing and qPCR indicated that the colonization of <i>P. indica</i> up-regulated the expression of genes involved in the phenylpropanoid biosynthesis pathway. The metabolome analysis indicated that the colonization of <i>P. indica</i> increased the metabolites’ accumulation in the phenylpropanoid biosynthesis. Consistent with transcriptome and metabolomic analysis, microscopic observations showed enhanced lignin accumulation in the roots of the Piri and Piri+Fp lines, most likely contributing to the arrested infection by <i>F. pseudograminearum.</i> These results suggested that <i>P. indica</i> increased resistance to <i>F. pseudograminearum</i> in wheat by inducing the phenylpropanoid pathway. |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-05c13e8d43a34f979a09f6a72d2a57302023-11-18T01:42:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410879710.3390/ijms24108797<i>Piriformospora indica</i> Increases Resistance to <i>Fusarium pseudograminearum</i> in Wheat by Inducing Phenylpropanoid PathwayLiang Li0Ruiying Hao1Xiurong Yang2Yu Feng3Zhenghui Bi4School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, ChinaSchool of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, ChinaInstitute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300401, ChinaSchool of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, ChinaSchool of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, ChinaFusarium crown rot (FCR), mainly caused by <i>Fusarium pseudograminearum</i>, not only seriously threatens the yield and quality of wheat, but also endangers the health and safety of humans and livestock. <i>Piriformospora indica</i> is a root endophytic fungus that colonizes plant roots extensively and can effectively promote plant growth and improve plant resistance to biotic and abiotic stresses. In this study, the mechanism of FCR resistance mediated by <i>P. indica</i> in wheat was revealed from the phenylpropanoid metabolic pathway. The results showed that the colonization of <i>P. indica</i> significantly reduced the progression of wheat disease, the amount of <i>F. pseudograminearum</i> colonization, and the content of deoxynivalenol (DON) in wheat roots. RNA-seq suggested that <i>P. indica</i> colonization could reduce the number of differentially expressed genes (DEGs) in the transcriptome caused by <i>F. pseudograminearum</i> infection. The DEGs induced by the colonization of <i>P. indica</i> were partially enriched in phenylpropanoid biosynthesis. Transcriptome sequencing and qPCR indicated that the colonization of <i>P. indica</i> up-regulated the expression of genes involved in the phenylpropanoid biosynthesis pathway. The metabolome analysis indicated that the colonization of <i>P. indica</i> increased the metabolites’ accumulation in the phenylpropanoid biosynthesis. Consistent with transcriptome and metabolomic analysis, microscopic observations showed enhanced lignin accumulation in the roots of the Piri and Piri+Fp lines, most likely contributing to the arrested infection by <i>F. pseudograminearum.</i> These results suggested that <i>P. indica</i> increased resistance to <i>F. pseudograminearum</i> in wheat by inducing the phenylpropanoid pathway.https://www.mdpi.com/1422-0067/24/10/8797<i>Fusarium pseudograminearum</i>fusarium crown rot<i>Piriformospora indica</i>phenylpropanoid pathway |
| spellingShingle | Liang Li Ruiying Hao Xiurong Yang Yu Feng Zhenghui Bi <i>Piriformospora indica</i> Increases Resistance to <i>Fusarium pseudograminearum</i> in Wheat by Inducing Phenylpropanoid Pathway International Journal of Molecular Sciences <i>Fusarium pseudograminearum</i> fusarium crown rot <i>Piriformospora indica</i> phenylpropanoid pathway |
| title | <i>Piriformospora indica</i> Increases Resistance to <i>Fusarium pseudograminearum</i> in Wheat by Inducing Phenylpropanoid Pathway |
| title_full | <i>Piriformospora indica</i> Increases Resistance to <i>Fusarium pseudograminearum</i> in Wheat by Inducing Phenylpropanoid Pathway |
| title_fullStr | <i>Piriformospora indica</i> Increases Resistance to <i>Fusarium pseudograminearum</i> in Wheat by Inducing Phenylpropanoid Pathway |
| title_full_unstemmed | <i>Piriformospora indica</i> Increases Resistance to <i>Fusarium pseudograminearum</i> in Wheat by Inducing Phenylpropanoid Pathway |
| title_short | <i>Piriformospora indica</i> Increases Resistance to <i>Fusarium pseudograminearum</i> in Wheat by Inducing Phenylpropanoid Pathway |
| title_sort | i piriformospora indica i increases resistance to i fusarium pseudograminearum i in wheat by inducing phenylpropanoid pathway |
| topic | <i>Fusarium pseudograminearum</i> fusarium crown rot <i>Piriformospora indica</i> phenylpropanoid pathway |
| url | https://www.mdpi.com/1422-0067/24/10/8797 |
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