A Novel Sample-to-Answer Visual Nucleic Acid Detection System for Adenovirus Detection
ABSTRACT Human adenoviruses (HAdVs) are common viruses that can cause local outbreaks in schools, communities and military camps, posing a huge threat to public health. An ideal POCT device for adenovirus detection in resource-limited settings is critical to control the spread of the virus. In this...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2023-06-01
|
| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.05170-22 |
| _version_ | 1827925131665604608 |
|---|---|
| author | Kui Sun Xiaodong Yang Yanan Wang Qun Guan Wenliang Fu Chao Zhang Qin Liu Wenzheng An Yongqi Zhao Weiwei Xing Donggang Xu |
| author_facet | Kui Sun Xiaodong Yang Yanan Wang Qun Guan Wenliang Fu Chao Zhang Qin Liu Wenzheng An Yongqi Zhao Weiwei Xing Donggang Xu |
| author_sort | Kui Sun |
| collection | DOAJ |
| description | ABSTRACT Human adenoviruses (HAdVs) are common viruses that can cause local outbreaks in schools, communities and military camps, posing a huge threat to public health. An ideal POCT device for adenovirus detection in resource-limited settings is critical to control the spread of the virus. In this study, we developed an integrated and electricity-independent sample-to-answer system that can complete nucleic acid extraction, amplification, and detection at room temperature. This system is suitable for field and on-site detection because of its rapidity, sensitivity, lack of contamination, and lack of requirements of high-precision instruments and skilled technicians. It consists of two separate modules, ALP FINA (alkaline lysis with the paper-based filtration isolation of nucleic acid) and SV RPA (sealed and visual recombinase polymerase amplification). The extraction efficiency of ALP FINA can reach 48 to 84%, which is close to that of the conventional centrifuge column. The detection sensitivity of SV RPA is close to 10 copies/μL of AdvB and AdvE without aerosol contamination after repeated operations. When SV RPA was applied to the detection of nasopharyngeal swab samples of 19 patients who were infected with AdvB or AdvE as well as 10 healthy volunteers, its sensitivity and specificity reached 100%, respectively. IMPORTANCE HAdV infections are readily transmittable and, in some instances, highly contagious. Early and rapid diagnosis is essential for disease control. In this work, we developed a portable, disposable, and modularized sample-to-answer detection system for AdvB and AdvE, which rendered the entire test to be completely independent of electricity and other laboratory infrastructure. Thus, this detection system can be applied in resource-limited settings, and it has the potential to be further developed as an early diagnosis method in the field. |
| first_indexed | 2024-03-13T05:20:50Z |
| format | Article |
| id | doaj.art-05c15e36d1aa489e8ad126cfb3b2fe28 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-13T05:20:50Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-05c15e36d1aa489e8ad126cfb3b2fe282023-06-15T13:18:31ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-06-0111310.1128/spectrum.05170-22A Novel Sample-to-Answer Visual Nucleic Acid Detection System for Adenovirus DetectionKui Sun0Xiaodong Yang1Yanan Wang2Qun Guan3Wenliang Fu4Chao Zhang5Qin Liu6Wenzheng An7Yongqi Zhao8Weiwei Xing9Donggang Xu10Beijing Institute of Basic Medical Sciences, Beijing, ChinaDepartment of General Surgery, the First Medical Center of Chinese PLA General Hospital, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaThe Fifth Medical Center of Chinese PLA General Hospital, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaABSTRACT Human adenoviruses (HAdVs) are common viruses that can cause local outbreaks in schools, communities and military camps, posing a huge threat to public health. An ideal POCT device for adenovirus detection in resource-limited settings is critical to control the spread of the virus. In this study, we developed an integrated and electricity-independent sample-to-answer system that can complete nucleic acid extraction, amplification, and detection at room temperature. This system is suitable for field and on-site detection because of its rapidity, sensitivity, lack of contamination, and lack of requirements of high-precision instruments and skilled technicians. It consists of two separate modules, ALP FINA (alkaline lysis with the paper-based filtration isolation of nucleic acid) and SV RPA (sealed and visual recombinase polymerase amplification). The extraction efficiency of ALP FINA can reach 48 to 84%, which is close to that of the conventional centrifuge column. The detection sensitivity of SV RPA is close to 10 copies/μL of AdvB and AdvE without aerosol contamination after repeated operations. When SV RPA was applied to the detection of nasopharyngeal swab samples of 19 patients who were infected with AdvB or AdvE as well as 10 healthy volunteers, its sensitivity and specificity reached 100%, respectively. IMPORTANCE HAdV infections are readily transmittable and, in some instances, highly contagious. Early and rapid diagnosis is essential for disease control. In this work, we developed a portable, disposable, and modularized sample-to-answer detection system for AdvB and AdvE, which rendered the entire test to be completely independent of electricity and other laboratory infrastructure. Thus, this detection system can be applied in resource-limited settings, and it has the potential to be further developed as an early diagnosis method in the field.https://journals.asm.org/doi/10.1128/spectrum.05170-22RPAsample to answerhuman adenovirusesaerosol contaminationNAAT |
| spellingShingle | Kui Sun Xiaodong Yang Yanan Wang Qun Guan Wenliang Fu Chao Zhang Qin Liu Wenzheng An Yongqi Zhao Weiwei Xing Donggang Xu A Novel Sample-to-Answer Visual Nucleic Acid Detection System for Adenovirus Detection Microbiology Spectrum RPA sample to answer human adenoviruses aerosol contamination NAAT |
| title | A Novel Sample-to-Answer Visual Nucleic Acid Detection System for Adenovirus Detection |
| title_full | A Novel Sample-to-Answer Visual Nucleic Acid Detection System for Adenovirus Detection |
| title_fullStr | A Novel Sample-to-Answer Visual Nucleic Acid Detection System for Adenovirus Detection |
| title_full_unstemmed | A Novel Sample-to-Answer Visual Nucleic Acid Detection System for Adenovirus Detection |
| title_short | A Novel Sample-to-Answer Visual Nucleic Acid Detection System for Adenovirus Detection |
| title_sort | novel sample to answer visual nucleic acid detection system for adenovirus detection |
| topic | RPA sample to answer human adenoviruses aerosol contamination NAAT |
| url | https://journals.asm.org/doi/10.1128/spectrum.05170-22 |
| work_keys_str_mv | AT kuisun anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT xiaodongyang anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT yananwang anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT qunguan anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT wenliangfu anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT chaozhang anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT qinliu anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT wenzhengan anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT yongqizhao anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT weiweixing anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT donggangxu anovelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT kuisun novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT xiaodongyang novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT yananwang novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT qunguan novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT wenliangfu novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT chaozhang novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT qinliu novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT wenzhengan novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT yongqizhao novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT weiweixing novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection AT donggangxu novelsampletoanswervisualnucleicaciddetectionsystemforadenovirusdetection |