Constant ratio between the genomic components of bipartite begomoviruses during infection and transmission
Abstract The genomic components of multipartite viruses are encapsidated in separate virus particles, and the frequencies of genomic components represent one of the key genetic features. Many begomoviruses of economic significance are bipartite, and the details of the association between their genom...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-023-02148-2 |
| _version_ | 1797453852302114816 |
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| author | Yu-Xin Xiao Di Li Yi-Jie Wu Shu-Sheng Liu Li-Long Pan |
| author_facet | Yu-Xin Xiao Di Li Yi-Jie Wu Shu-Sheng Liu Li-Long Pan |
| author_sort | Yu-Xin Xiao |
| collection | DOAJ |
| description | Abstract The genomic components of multipartite viruses are encapsidated in separate virus particles, and the frequencies of genomic components represent one of the key genetic features. Many begomoviruses of economic significance are bipartite, and the details of the association between their genomic components remain largely unexplored. We first analyzed the temporal dynamics of the quantities of DNA-A and DNA-B and the B/A ratio of the squash leaf curl China virus (SLCCNV) in plants and found that while the quantities of DNA-A and DNA-B varied significantly during infection, the B/A ratio remained constant. We then found that changes in the B/A ratio in agrobacteria inoculum may significantly alter the B/A ratio in plants at 6 days post inoculation, but the differences disappeared shortly thereafter. We next showed that while the quantities of DNA-A and DNA-B among plants infected by agrobacteria, sap transmission and whitefly-mediated transmission differed significantly, the B/A ratios were similar. Further analysis of gene expression revealed that the ratio of the expression of genes encoded by DNA-A and DNA-B varied significantly during infection. Finally, we monitored the temporal dynamics of the quantities of DNA-A and DNA-B and the B/A ratio of another bipartite begomovirus, and a constant B/A ratio was similarly observed. Our findings highlight the maintenance of a constant ratio between the two genomic components of bipartite begomoviruses during infection and transmission, and provide new insights into the biology of begomoviruses. |
| first_indexed | 2024-03-09T15:28:51Z |
| format | Article |
| id | doaj.art-05c1a6fa79754657b1615ccb643630d1 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-03-09T15:28:51Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-05c1a6fa79754657b1615ccb643630d12023-11-26T12:20:39ZengBMCVirology Journal1743-422X2023-08-0120111010.1186/s12985-023-02148-2Constant ratio between the genomic components of bipartite begomoviruses during infection and transmissionYu-Xin Xiao0Di Li1Yi-Jie Wu2Shu-Sheng Liu3Li-Long Pan4Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang UniversityMinistry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang UniversityMinistry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang UniversityMinistry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang UniversityMinistry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang UniversityAbstract The genomic components of multipartite viruses are encapsidated in separate virus particles, and the frequencies of genomic components represent one of the key genetic features. Many begomoviruses of economic significance are bipartite, and the details of the association between their genomic components remain largely unexplored. We first analyzed the temporal dynamics of the quantities of DNA-A and DNA-B and the B/A ratio of the squash leaf curl China virus (SLCCNV) in plants and found that while the quantities of DNA-A and DNA-B varied significantly during infection, the B/A ratio remained constant. We then found that changes in the B/A ratio in agrobacteria inoculum may significantly alter the B/A ratio in plants at 6 days post inoculation, but the differences disappeared shortly thereafter. We next showed that while the quantities of DNA-A and DNA-B among plants infected by agrobacteria, sap transmission and whitefly-mediated transmission differed significantly, the B/A ratios were similar. Further analysis of gene expression revealed that the ratio of the expression of genes encoded by DNA-A and DNA-B varied significantly during infection. Finally, we monitored the temporal dynamics of the quantities of DNA-A and DNA-B and the B/A ratio of another bipartite begomovirus, and a constant B/A ratio was similarly observed. Our findings highlight the maintenance of a constant ratio between the two genomic components of bipartite begomoviruses during infection and transmission, and provide new insights into the biology of begomoviruses.https://doi.org/10.1186/s12985-023-02148-2Bipartite begomovirusRatio between DNA-B and DNA-AInfectionTransmissionSquash leaf curl China virusSri Lankan cassava mosaic virus |
| spellingShingle | Yu-Xin Xiao Di Li Yi-Jie Wu Shu-Sheng Liu Li-Long Pan Constant ratio between the genomic components of bipartite begomoviruses during infection and transmission Virology Journal Bipartite begomovirus Ratio between DNA-B and DNA-A Infection Transmission Squash leaf curl China virus Sri Lankan cassava mosaic virus |
| title | Constant ratio between the genomic components of bipartite begomoviruses during infection and transmission |
| title_full | Constant ratio between the genomic components of bipartite begomoviruses during infection and transmission |
| title_fullStr | Constant ratio between the genomic components of bipartite begomoviruses during infection and transmission |
| title_full_unstemmed | Constant ratio between the genomic components of bipartite begomoviruses during infection and transmission |
| title_short | Constant ratio between the genomic components of bipartite begomoviruses during infection and transmission |
| title_sort | constant ratio between the genomic components of bipartite begomoviruses during infection and transmission |
| topic | Bipartite begomovirus Ratio between DNA-B and DNA-A Infection Transmission Squash leaf curl China virus Sri Lankan cassava mosaic virus |
| url | https://doi.org/10.1186/s12985-023-02148-2 |
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