The Optimized Fabrication of a Novel Nanobubble for Tumor Imaging
Nanobubbles with a size of less than 1 µm can be used as ultrasound contrast agents for diagnosis and as drug/gene carriers for therapy. However, the optimal method of preparing uniform-sized nanobubbles is considered controversial. In this study, we developed novel biocompatible nanobubbles by perf...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.00610/full |
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| author | Jiaqi Zhang Jiaqi Zhang Yihan Chen Yihan Chen Cheng Deng Cheng Deng Li Zhang Li Zhang Zhenxing Sun Zhenxing Sun Jing Wang Jing Wang Yali Yang Yali Yang Qing Lv Qing Lv Wei Han Wei Han Mingxing Xie Mingxing Xie |
| author_facet | Jiaqi Zhang Jiaqi Zhang Yihan Chen Yihan Chen Cheng Deng Cheng Deng Li Zhang Li Zhang Zhenxing Sun Zhenxing Sun Jing Wang Jing Wang Yali Yang Yali Yang Qing Lv Qing Lv Wei Han Wei Han Mingxing Xie Mingxing Xie |
| author_sort | Jiaqi Zhang |
| collection | DOAJ |
| description | Nanobubbles with a size of less than 1 µm can be used as ultrasound contrast agents for diagnosis and as drug/gene carriers for therapy. However, the optimal method of preparing uniform-sized nanobubbles is considered controversial. In this study, we developed novel biocompatible nanobubbles by performing differential centrifugation to isolate the relevant subpopulation from the parent suspensions. Compared with the method of modulating the thickness of the phospholipid film without centrifugation, nanobubbles fabricated under optimal centrifugation conditions exhibited a uniform bubble size, good stability, and low toxicity. Using in vitro ultrasound imaging, nanobubbles displayed excellent enhancement ability, which was comparable to microbubbles. In an in vivo experiment, the video intensity of nanobubbles in tumors was stronger than that of microbubbles at different times (5 min, 163.5 ± 8.3 a.u. vs. 143.2 ± 7.5 a.u., P < 0.01; 15 min, 125.4 ± 5.2 a.u. vs. 97.3 ± 4.6 a.u., P < 0.01). Fluorescence imaging obtained by confocal laser scanning microscopy demonstrated that obviously more nanobubbles passed through the vessel wall into the extravascular and intercellular space of tumors, compared with microbubbles. In conclusion, this optimized preparation method will strongly promote the application of nanobubbles in imaging and therapy. |
| first_indexed | 2024-12-11T23:39:23Z |
| format | Article |
| id | doaj.art-05c2ea54499d49e083ad80b89049c6c0 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T23:39:23Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-05c2ea54499d49e083ad80b89049c6c02022-12-22T00:45:46ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-05-011010.3389/fphar.2019.00610451396The Optimized Fabrication of a Novel Nanobubble for Tumor ImagingJiaqi Zhang0Jiaqi Zhang1Yihan Chen2Yihan Chen3Cheng Deng4Cheng Deng5Li Zhang6Li Zhang7Zhenxing Sun8Zhenxing Sun9Jing Wang10Jing Wang11Yali Yang12Yali Yang13Qing Lv14Qing Lv15Wei Han16Wei Han17Mingxing Xie18Mingxing Xie19Department of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaDepartment of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaDepartment of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaDepartment of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaDepartment of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaDepartment of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaDepartment of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaDepartment of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaDepartment of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaDepartment of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Key Lab of Molecular Imaging, Wuhan, ChinaNanobubbles with a size of less than 1 µm can be used as ultrasound contrast agents for diagnosis and as drug/gene carriers for therapy. However, the optimal method of preparing uniform-sized nanobubbles is considered controversial. In this study, we developed novel biocompatible nanobubbles by performing differential centrifugation to isolate the relevant subpopulation from the parent suspensions. Compared with the method of modulating the thickness of the phospholipid film without centrifugation, nanobubbles fabricated under optimal centrifugation conditions exhibited a uniform bubble size, good stability, and low toxicity. Using in vitro ultrasound imaging, nanobubbles displayed excellent enhancement ability, which was comparable to microbubbles. In an in vivo experiment, the video intensity of nanobubbles in tumors was stronger than that of microbubbles at different times (5 min, 163.5 ± 8.3 a.u. vs. 143.2 ± 7.5 a.u., P < 0.01; 15 min, 125.4 ± 5.2 a.u. vs. 97.3 ± 4.6 a.u., P < 0.01). Fluorescence imaging obtained by confocal laser scanning microscopy demonstrated that obviously more nanobubbles passed through the vessel wall into the extravascular and intercellular space of tumors, compared with microbubbles. In conclusion, this optimized preparation method will strongly promote the application of nanobubbles in imaging and therapy.https://www.frontiersin.org/article/10.3389/fphar.2019.00610/fullnanobubblesultrasoundcontrast imagingtumorfabrication |
| spellingShingle | Jiaqi Zhang Jiaqi Zhang Yihan Chen Yihan Chen Cheng Deng Cheng Deng Li Zhang Li Zhang Zhenxing Sun Zhenxing Sun Jing Wang Jing Wang Yali Yang Yali Yang Qing Lv Qing Lv Wei Han Wei Han Mingxing Xie Mingxing Xie The Optimized Fabrication of a Novel Nanobubble for Tumor Imaging Frontiers in Pharmacology nanobubbles ultrasound contrast imaging tumor fabrication |
| title | The Optimized Fabrication of a Novel Nanobubble for Tumor Imaging |
| title_full | The Optimized Fabrication of a Novel Nanobubble for Tumor Imaging |
| title_fullStr | The Optimized Fabrication of a Novel Nanobubble for Tumor Imaging |
| title_full_unstemmed | The Optimized Fabrication of a Novel Nanobubble for Tumor Imaging |
| title_short | The Optimized Fabrication of a Novel Nanobubble for Tumor Imaging |
| title_sort | optimized fabrication of a novel nanobubble for tumor imaging |
| topic | nanobubbles ultrasound contrast imaging tumor fabrication |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.00610/full |
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