A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer
Abstract Background Colon cancer (CC) is a common malignant cancer. Recently, circFNDC3B was found to exert biological function in multiple cancers. However, it was unclear whether the potential protein encoded by circFNDC3B is involved in carcinogenesis of CC. Methods We used Sanger sequence and RN...
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | Molecular Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12943-020-01179-5 |
| _version_ | 1818027923478675456 |
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| author | Zihao Pan Jianye Cai Jiatong Lin Huinian Zhou Jingwen Peng Jinliang Liang Long Xia Qi Yin Baojia Zou Jun Zheng Liang Qiao Lei Zhang |
| author_facet | Zihao Pan Jianye Cai Jiatong Lin Huinian Zhou Jingwen Peng Jinliang Liang Long Xia Qi Yin Baojia Zou Jun Zheng Liang Qiao Lei Zhang |
| author_sort | Zihao Pan |
| collection | DOAJ |
| description | Abstract Background Colon cancer (CC) is a common malignant cancer. Recently, circFNDC3B was found to exert biological function in multiple cancers. However, it was unclear whether the potential protein encoded by circFNDC3B is involved in carcinogenesis of CC. Methods We used Sanger sequence and RNase R digestion assay to confirm the existence of circFNDC3B, and quantitative real-time PCR was used to evaluate the circRNA’s expression. Then fluorescence in situ hybridization (FISH) was performed to study location of circFNDC3B. The identification of protein encoded by circFNDC3B was performed using LC-MS/MS. The function of circFNDC3B-218aa on proliferation, invasion and migration were assessed by CCK8 assays, colony formation assays, transwell assays, wound-healing assays and animal experiments. RNA-sequencing and western blot were used to identify the gene regulated by circFNDC3B-218aa. Finally, glucose metabolism-related assays were performed to further investigate function of circFNDC3B-218aa. Results CircFNDC3B was localized mostly in the cytoplasm, and was decreased in CC cell lines and tissues. The patients with low circFNDC3B expression had a shorter OS (P = 0.0014) than patients with high expression. Moreover, circFNDC3B inhibited the proliferation, invasion and migration of CC cells. Next, we identified that circFNDC3B could encode a novel protein circFNDC3B-218aa. Furthermore, circFNDC3B-218aa, not circFNDC3B, inhibited the proliferation, invasion and migration of CC. Additionally, the in vivo experiments implied that up-regulated circFNDC3B-218aa exhibited an inhibitory effect on CC progression. By RNA-sequencing, western blot and glucose metabolism-related assays, we found that circFNDC3B-218aa inhibited the expression of Snail, and subsequently promoted the tumor-suppressive effect of FBP1 in CC. Conclusions The novel circFNDC3B-218aa may serve as a tumor suppressive factor and potential biomarker which may supply the potential therapeutic target for CC. |
| first_indexed | 2024-12-10T04:55:37Z |
| format | Article |
| id | doaj.art-05c8955b461640daa98ffa4c468e7a34 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-12-10T04:55:37Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-05c8955b461640daa98ffa4c468e7a342022-12-22T02:01:30ZengBMCMolecular Cancer1476-45982020-04-0119111510.1186/s12943-020-01179-5A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancerZihao Pan0Jianye Cai1Jiatong Lin2Huinian Zhou3Jingwen Peng4Jinliang Liang5Long Xia6Qi Yin7Baojia Zou8Jun Zheng9Liang Qiao10Lei Zhang11Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen UniversityDepartment of Hepatic Surgery and Liver Transplantation Center, Department of Hepatic Surgery and Liver Transplantation Center, The Third Affiliated Hospital, Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen UniversityDepartment of General Surgery, Lanzhou University Second HospitalGuangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen UniversityGuangdong Key Laboratory of Liver Disease Research, Key Laboratory of Liver Disease Biotherapy and Translational Medicine of Guangdong Higher Education Institutes, The Third Affiliated Hospital, Sun Yat-sen UniversityDepartment of Hepatic Surgery and Liver Transplantation Center, Department of Hepatic Surgery and Liver Transplantation Center, The Third Affiliated Hospital, Sun Yat-sen UniversityCookGen Biosciences CenterDepartment of Hepatobiliary Surgery, The Fifth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Hepatic Surgery and Liver Transplantation Center, Department of Hepatic Surgery and Liver Transplantation Center, The Third Affiliated Hospital, Sun Yat-sen UniversityStorr Liver Centre, Westmead Institute for Medical Research, University of Sydney at Westmead HospitalDepartment of Biliary-Pancreatic Surgery, The Third Affiliated Hospital, Sun Yat-sen UniversityAbstract Background Colon cancer (CC) is a common malignant cancer. Recently, circFNDC3B was found to exert biological function in multiple cancers. However, it was unclear whether the potential protein encoded by circFNDC3B is involved in carcinogenesis of CC. Methods We used Sanger sequence and RNase R digestion assay to confirm the existence of circFNDC3B, and quantitative real-time PCR was used to evaluate the circRNA’s expression. Then fluorescence in situ hybridization (FISH) was performed to study location of circFNDC3B. The identification of protein encoded by circFNDC3B was performed using LC-MS/MS. The function of circFNDC3B-218aa on proliferation, invasion and migration were assessed by CCK8 assays, colony formation assays, transwell assays, wound-healing assays and animal experiments. RNA-sequencing and western blot were used to identify the gene regulated by circFNDC3B-218aa. Finally, glucose metabolism-related assays were performed to further investigate function of circFNDC3B-218aa. Results CircFNDC3B was localized mostly in the cytoplasm, and was decreased in CC cell lines and tissues. The patients with low circFNDC3B expression had a shorter OS (P = 0.0014) than patients with high expression. Moreover, circFNDC3B inhibited the proliferation, invasion and migration of CC cells. Next, we identified that circFNDC3B could encode a novel protein circFNDC3B-218aa. Furthermore, circFNDC3B-218aa, not circFNDC3B, inhibited the proliferation, invasion and migration of CC. Additionally, the in vivo experiments implied that up-regulated circFNDC3B-218aa exhibited an inhibitory effect on CC progression. By RNA-sequencing, western blot and glucose metabolism-related assays, we found that circFNDC3B-218aa inhibited the expression of Snail, and subsequently promoted the tumor-suppressive effect of FBP1 in CC. Conclusions The novel circFNDC3B-218aa may serve as a tumor suppressive factor and potential biomarker which may supply the potential therapeutic target for CC.http://link.springer.com/article/10.1186/s12943-020-01179-5circRNAcircFNDC3BFBP1Colon cancer |
| spellingShingle | Zihao Pan Jianye Cai Jiatong Lin Huinian Zhou Jingwen Peng Jinliang Liang Long Xia Qi Yin Baojia Zou Jun Zheng Liang Qiao Lei Zhang A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer Molecular Cancer circRNA circFNDC3B FBP1 Colon cancer |
| title | A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer |
| title_full | A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer |
| title_fullStr | A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer |
| title_full_unstemmed | A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer |
| title_short | A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer |
| title_sort | novel protein encoded by circfndc3b inhibits tumor progression and emt through regulating snail in colon cancer |
| topic | circRNA circFNDC3B FBP1 Colon cancer |
| url | http://link.springer.com/article/10.1186/s12943-020-01179-5 |
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