Analgesic and anti-inflammatory effects of diclofenac and ketoprofen patches in two different rat models of acute inflammation
Objectives: Topical forms of Non-steroidal anti-inflammatory drugs (NSAIDs) used to treat pain, fever and inflammation ensure reduced occurrence of systemic adverse effects that are common with oral application. Compared to the systemic therapy, topical applications of NSAIDs require lower doses to...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722005754 |
| _version_ | 1811178099161694208 |
|---|---|
| author | S. Maleškić Kapo M. Rakanović-Todić L. Burnazović-Ristić J. Kusturica A. Kulo Ćesić E. Ademović S. Loga-Zec A. Sarač-Hadžihalilović I. Aganović-Mušinović |
| author_facet | S. Maleškić Kapo M. Rakanović-Todić L. Burnazović-Ristić J. Kusturica A. Kulo Ćesić E. Ademović S. Loga-Zec A. Sarač-Hadžihalilović I. Aganović-Mušinović |
| author_sort | S. Maleškić Kapo |
| collection | DOAJ |
| description | Objectives: Topical forms of Non-steroidal anti-inflammatory drugs (NSAIDs) used to treat pain, fever and inflammation ensure reduced occurrence of systemic adverse effects that are common with oral application. Compared to the systemic therapy, topical applications of NSAIDs require lower doses to achieve pain relief, and thus pose lower risk of major drug interactions. We aimed to evaluate analgesic and anti-inflammatory effects of diclofenac and ketoprofen patch in the rat model of acute inflammation. Methods: Wistar male rats, body weight 220–290 g were randomized into five groups with six rats. Two groups were treated with diclofenac patch at different doses, two with ketoprofen patch at different doses, while a placebo patch was administered to the control group. After patches removal and initial measurements of pain, gait, body temperature, paw temperature, color and paw volume, the models of acute inflammation were applied: yeast-induced hyperalgesia and carrageenan-induced edema. Measurements were repeated one, three and five hours after inflammation induction. Results: Acute inflammations induced by both used models did not affect the whole-body temperature. However, there were significant (p < 0.05) differences in pain, gait, paw temperature, color and volume between low and high dose diclofenac and ketoprofen groups compared to the control group in different time points, in both models. Conclusions: Our results confirm the analgesic and anti-inflammatory efficacy of diclofenac and ketoprofen patches when used in two well-established rat models of inflammation. These findings suggests that diclofenac patch would be a useful formulation in clinical practice as a novel treatment option. However, new prospective double blind randomized clinical trials are necessary to evaluate analgesic and anti-inflammatory effect of topically applied NSAIDs when used in specific pain and inflammation indications. |
| first_indexed | 2024-04-11T06:13:44Z |
| format | Article |
| id | doaj.art-05cc3341866349f4b88bd7f14126fcb2 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-11T06:13:44Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-05cc3341866349f4b88bd7f14126fcb22022-12-22T04:41:08ZengElsevierJournal of King Saud University: Science1018-36472023-01-01351102394Analgesic and anti-inflammatory effects of diclofenac and ketoprofen patches in two different rat models of acute inflammationS. Maleškić Kapo0M. Rakanović-Todić1L. Burnazović-Ristić2J. Kusturica3A. Kulo Ćesić4E. Ademović5S. Loga-Zec6A. Sarač-Hadžihalilović7I. Aganović-Mušinović8Corresponding author.; University of Sarajevo - Medical faculty, Čekaluša 90, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Medical faculty, Čekaluša 90, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Medical faculty, Čekaluša 90, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Medical faculty, Čekaluša 90, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Medical faculty, Čekaluša 90, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Medical faculty, Čekaluša 90, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Medical faculty, Čekaluša 90, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Medical faculty, Čekaluša 90, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Medical faculty, Čekaluša 90, Sarajevo, Bosnia and HerzegovinaObjectives: Topical forms of Non-steroidal anti-inflammatory drugs (NSAIDs) used to treat pain, fever and inflammation ensure reduced occurrence of systemic adverse effects that are common with oral application. Compared to the systemic therapy, topical applications of NSAIDs require lower doses to achieve pain relief, and thus pose lower risk of major drug interactions. We aimed to evaluate analgesic and anti-inflammatory effects of diclofenac and ketoprofen patch in the rat model of acute inflammation. Methods: Wistar male rats, body weight 220–290 g were randomized into five groups with six rats. Two groups were treated with diclofenac patch at different doses, two with ketoprofen patch at different doses, while a placebo patch was administered to the control group. After patches removal and initial measurements of pain, gait, body temperature, paw temperature, color and paw volume, the models of acute inflammation were applied: yeast-induced hyperalgesia and carrageenan-induced edema. Measurements were repeated one, three and five hours after inflammation induction. Results: Acute inflammations induced by both used models did not affect the whole-body temperature. However, there were significant (p < 0.05) differences in pain, gait, paw temperature, color and volume between low and high dose diclofenac and ketoprofen groups compared to the control group in different time points, in both models. Conclusions: Our results confirm the analgesic and anti-inflammatory efficacy of diclofenac and ketoprofen patches when used in two well-established rat models of inflammation. These findings suggests that diclofenac patch would be a useful formulation in clinical practice as a novel treatment option. However, new prospective double blind randomized clinical trials are necessary to evaluate analgesic and anti-inflammatory effect of topically applied NSAIDs when used in specific pain and inflammation indications.http://www.sciencedirect.com/science/article/pii/S1018364722005754InflammationHyperalgesiaDiclofenacKetoprofenRat |
| spellingShingle | S. Maleškić Kapo M. Rakanović-Todić L. Burnazović-Ristić J. Kusturica A. Kulo Ćesić E. Ademović S. Loga-Zec A. Sarač-Hadžihalilović I. Aganović-Mušinović Analgesic and anti-inflammatory effects of diclofenac and ketoprofen patches in two different rat models of acute inflammation Journal of King Saud University: Science Inflammation Hyperalgesia Diclofenac Ketoprofen Rat |
| title | Analgesic and anti-inflammatory effects of diclofenac and ketoprofen patches in two different rat models of acute inflammation |
| title_full | Analgesic and anti-inflammatory effects of diclofenac and ketoprofen patches in two different rat models of acute inflammation |
| title_fullStr | Analgesic and anti-inflammatory effects of diclofenac and ketoprofen patches in two different rat models of acute inflammation |
| title_full_unstemmed | Analgesic and anti-inflammatory effects of diclofenac and ketoprofen patches in two different rat models of acute inflammation |
| title_short | Analgesic and anti-inflammatory effects of diclofenac and ketoprofen patches in two different rat models of acute inflammation |
| title_sort | analgesic and anti inflammatory effects of diclofenac and ketoprofen patches in two different rat models of acute inflammation |
| topic | Inflammation Hyperalgesia Diclofenac Ketoprofen Rat |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722005754 |
| work_keys_str_mv | AT smaleskickapo analgesicandantiinflammatoryeffectsofdiclofenacandketoprofenpatchesintwodifferentratmodelsofacuteinflammation AT mrakanovictodic analgesicandantiinflammatoryeffectsofdiclofenacandketoprofenpatchesintwodifferentratmodelsofacuteinflammation AT lburnazovicristic analgesicandantiinflammatoryeffectsofdiclofenacandketoprofenpatchesintwodifferentratmodelsofacuteinflammation AT jkusturica analgesicandantiinflammatoryeffectsofdiclofenacandketoprofenpatchesintwodifferentratmodelsofacuteinflammation AT akulocesic analgesicandantiinflammatoryeffectsofdiclofenacandketoprofenpatchesintwodifferentratmodelsofacuteinflammation AT eademovic analgesicandantiinflammatoryeffectsofdiclofenacandketoprofenpatchesintwodifferentratmodelsofacuteinflammation AT slogazec analgesicandantiinflammatoryeffectsofdiclofenacandketoprofenpatchesintwodifferentratmodelsofacuteinflammation AT asarachadzihalilovic analgesicandantiinflammatoryeffectsofdiclofenacandketoprofenpatchesintwodifferentratmodelsofacuteinflammation AT iaganovicmusinovic analgesicandantiinflammatoryeffectsofdiclofenacandketoprofenpatchesintwodifferentratmodelsofacuteinflammation |