A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha
The description and evaluation of morphological features are essential to many biological studies. Bioimaging and quantification methods have been developed to analyze the morphological features of plants. However, efficient three-dimensional (3D) imaging and its quantification are still under devel...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.01214/full |
| _version_ | 1818840519949680640 |
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| author | Tomoyuki Furuya Yoshitaka Kimori Yoshitaka Kimori Hirokazu Tsukaya Hirokazu Tsukaya |
| author_facet | Tomoyuki Furuya Yoshitaka Kimori Yoshitaka Kimori Hirokazu Tsukaya Hirokazu Tsukaya |
| author_sort | Tomoyuki Furuya |
| collection | DOAJ |
| description | The description and evaluation of morphological features are essential to many biological studies. Bioimaging and quantification methods have been developed to analyze the morphological features of plants. However, efficient three-dimensional (3D) imaging and its quantification are still under development, particularly for studies of plant morphology, due to complex organ structure with great flexibility among individuals with the same genotype. In this study, we propose a new approach that combines a 3D imaging technique using micro-computed tomography and a mathematical image-processing method to describe 3D morphological features. As an example, we applied this method to Marchantia polymorpha, a new model plant used for the evolutional study of land plants, and we evaluated a mutant individual with an abnormal 3D shape. Using this new method, we quantitatively described the thallus morphology of M. polymorpha and distinguished the wild type from a mutant with different morphological features. Our newly established method can be applied to various tissues or bodies with irregular 3D morphology. |
| first_indexed | 2024-12-19T04:11:29Z |
| format | Article |
| id | doaj.art-05cd9e56d8e345ab88575a4e09a158a7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T04:11:29Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-05cd9e56d8e345ab88575a4e09a158a72022-12-21T20:36:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-10-011010.3389/fpls.2019.01214471609A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorphaTomoyuki Furuya0Yoshitaka Kimori1Yoshitaka Kimori2Hirokazu Tsukaya3Hirokazu Tsukaya4Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, JapanDepartment of Imaging Science, Center for Novel Science Initiatives, National Institutes of Natural Sciences, Okazaki, JapanDepartment of Management and Information Sciences, Faculty of Environmental and Information Sciences, Fukui University of Technology, Fukui, JapanDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, JapanExCELLS, National Institutes of Natural Sciences, Okazaki, JapanThe description and evaluation of morphological features are essential to many biological studies. Bioimaging and quantification methods have been developed to analyze the morphological features of plants. However, efficient three-dimensional (3D) imaging and its quantification are still under development, particularly for studies of plant morphology, due to complex organ structure with great flexibility among individuals with the same genotype. In this study, we propose a new approach that combines a 3D imaging technique using micro-computed tomography and a mathematical image-processing method to describe 3D morphological features. As an example, we applied this method to Marchantia polymorpha, a new model plant used for the evolutional study of land plants, and we evaluated a mutant individual with an abnormal 3D shape. Using this new method, we quantitatively described the thallus morphology of M. polymorpha and distinguished the wild type from a mutant with different morphological features. Our newly established method can be applied to various tissues or bodies with irregular 3D morphology.https://www.frontiersin.org/article/10.3389/fpls.2019.01214/fullliverwortsMarchantia polymorphathree-dimensional imagingmicro-computed tomographymathematical image processing |
| spellingShingle | Tomoyuki Furuya Yoshitaka Kimori Yoshitaka Kimori Hirokazu Tsukaya Hirokazu Tsukaya A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha Frontiers in Plant Science liverworts Marchantia polymorpha three-dimensional imaging micro-computed tomography mathematical image processing |
| title | A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha |
| title_full | A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha |
| title_fullStr | A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha |
| title_full_unstemmed | A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha |
| title_short | A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha |
| title_sort | method for evaluating three dimensional morphological features a case study using marchantia polymorpha |
| topic | liverworts Marchantia polymorpha three-dimensional imaging micro-computed tomography mathematical image processing |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.01214/full |
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