Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic Effects
Label-free and real-time monitoring of the bacterial viability is essential for the accurate and sensitive characterization of the antibiotic effects. In the present study, we investigated the feasibility of the interdigitated and wave-shaped electrode (IWE) for monitoring the effect of tetracycline...
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MDPI AG
2020-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/18/5237 |
| _version_ | 1797553690717978624 |
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| author | Jinsoo Park Yonghyun Lee Youjin Hwang Sungbo Cho |
| author_facet | Jinsoo Park Yonghyun Lee Youjin Hwang Sungbo Cho |
| author_sort | Jinsoo Park |
| collection | DOAJ |
| description | Label-free and real-time monitoring of the bacterial viability is essential for the accurate and sensitive characterization of the antibiotic effects. In the present study, we investigated the feasibility of the interdigitated and wave-shaped electrode (IWE) for monitoring the effect of tetracycline or kanamycin on <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and methicillin-resistant <i>S.</i><i>aureus</i> (MRSA). The electrical impedance spectra of the IWE immersed in the culture media for bacterial growth were characterized in a frequency range of 10 Hz to 1 kHz. The capacitance index (CI) (capacitance change relevant with the bacterial viability) was used to monitor the antibiotic effects on the <i>S</i>. <i>aureus</i> and MRSA in comparison to the traditional methods (disk diffusion test and optical density (OD) measurement). The experimental results showed that the percentage of change in CI (PCI) for the antibiotic effect on MRSA was increased by 51.58% and 57.83% in kanamycin and control, respectively. In contrast, the PCI value decreased by 0.25% for tetracycline, decreased by 52.63% and 37.66% in the cases of tetracycline and kanamycin-treated <i>S. aureus</i>, and increased 2.79% in the control, respectively. This study demonstrated the feasibility of the IWE-based capacitance sensor for the label-free and real-time monitoring of the antibiotic effects on <i>S. aureus</i> and MRSA. |
| first_indexed | 2024-03-10T16:20:10Z |
| format | Article |
| id | doaj.art-05ce315ec87e4f889a02f96a88a984c7 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T16:20:10Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-05ce315ec87e4f889a02f96a88a984c72023-11-20T13:43:28ZengMDPI AGSensors1424-82202020-09-012018523710.3390/s20185237Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic EffectsJinsoo Park0Yonghyun Lee1Youjin Hwang2Sungbo Cho3Department of Health Science and Technology, GAIHST, Gachon University, Incheon 21999, KoreaDepartment of Health Science and Technology, GAIHST, Gachon University, Incheon 21999, KoreaDepartment of Health Science and Technology, GAIHST, Gachon University, Incheon 21999, KoreaDepartment of Health Science and Technology, GAIHST, Gachon University, Incheon 21999, KoreaLabel-free and real-time monitoring of the bacterial viability is essential for the accurate and sensitive characterization of the antibiotic effects. In the present study, we investigated the feasibility of the interdigitated and wave-shaped electrode (IWE) for monitoring the effect of tetracycline or kanamycin on <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and methicillin-resistant <i>S.</i><i>aureus</i> (MRSA). The electrical impedance spectra of the IWE immersed in the culture media for bacterial growth were characterized in a frequency range of 10 Hz to 1 kHz. The capacitance index (CI) (capacitance change relevant with the bacterial viability) was used to monitor the antibiotic effects on the <i>S</i>. <i>aureus</i> and MRSA in comparison to the traditional methods (disk diffusion test and optical density (OD) measurement). The experimental results showed that the percentage of change in CI (PCI) for the antibiotic effect on MRSA was increased by 51.58% and 57.83% in kanamycin and control, respectively. In contrast, the PCI value decreased by 0.25% for tetracycline, decreased by 52.63% and 37.66% in the cases of tetracycline and kanamycin-treated <i>S. aureus</i>, and increased 2.79% in the control, respectively. This study demonstrated the feasibility of the IWE-based capacitance sensor for the label-free and real-time monitoring of the antibiotic effects on <i>S. aureus</i> and MRSA.https://www.mdpi.com/1424-8220/20/18/5237antibioticsbacterial viabilitycapacitance sensorimpedance spectroscopylabel-freereal time |
| spellingShingle | Jinsoo Park Yonghyun Lee Youjin Hwang Sungbo Cho Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic Effects Sensors antibiotics bacterial viability capacitance sensor impedance spectroscopy label-free real time |
| title | Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic Effects |
| title_full | Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic Effects |
| title_fullStr | Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic Effects |
| title_full_unstemmed | Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic Effects |
| title_short | Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic Effects |
| title_sort | interdigitated and wave shaped electrode based capacitance sensor for monitoring antibiotic effects |
| topic | antibiotics bacterial viability capacitance sensor impedance spectroscopy label-free real time |
| url | https://www.mdpi.com/1424-8220/20/18/5237 |
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