Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous Cycle
Oestrogen influences autonomic function via actions at classical nuclear oestrogen receptors α and β in the brain, and recent evidence suggests the orphan G protein-coupled receptor GPR30 may also function as a cytoplasmic oestrogen receptor. We investigated the expression of GPR30 in female rat bra...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2012-02-01
|
| Series: | Neurosignals |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/333296 |
| _version_ | 1819092561239736320 |
|---|---|
| author | Emma J. Spary Sally E. Chapman John K. Sinfield Azhar Maqbool Jean Kaye Trevor F.C. Batten |
| author_facet | Emma J. Spary Sally E. Chapman John K. Sinfield Azhar Maqbool Jean Kaye Trevor F.C. Batten |
| author_sort | Emma J. Spary |
| collection | DOAJ |
| description | Oestrogen influences autonomic function via actions at classical nuclear oestrogen receptors α and β in the brain, and recent evidence suggests the orphan G protein-coupled receptor GPR30 may also function as a cytoplasmic oestrogen receptor. We investigated the expression of GPR30 in female rat brains throughout the oestrous cycle and after ovariectomy to determine whether GPR30 expression in central autonomic nuclei is correlated with circulating oestrogen levels. In the nucleus of the solitary tract (NTS), ventrolateral medulla (VLM) and periaqueductal gray (PAG) GPR30 mRNA, quantified by real-time PCR, was increased in proestrus and oestrus. In ovariectomised (OVX) rats, expression in NTS and VLM appeared increased compared to metoestrus, but in the hypothalamic paraventricular nucleus and PAG lower mRNA levels were seen in OVX. GPR30-like immunoreactivity (GPR30-LI) colocalised with Golgi in neurones in many brain areas associated with autonomic pathways, and analysis of numbers of immunoreactive neurones showed differences consistent with the PCR data. GPR30-LI was found in a variety of transmitter phenotypes, including cholinergic, serotonergic, catecholaminergic and nitrergic neurones in different neuronal groups. These observations support the view that GPR30 could act as a rapid transducer responding to oestrogen levels and thus modulate the activity of central autonomic pathways. |
| first_indexed | 2024-12-21T22:57:34Z |
| format | Article |
| id | doaj.art-05d142f2b2b8481f8a8a198cf6cef8a4 |
| institution | Directory Open Access Journal |
| issn | 1424-862X 1424-8638 |
| language | English |
| last_indexed | 2024-12-21T22:57:34Z |
| publishDate | 2012-02-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Neurosignals |
| spelling | doaj.art-05d142f2b2b8481f8a8a198cf6cef8a42022-12-21T18:47:24ZengCell Physiol Biochem Press GmbH & Co KGNeurosignals1424-862X1424-86382012-02-01211-2142710.1159/000333296333296Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous CycleEmma J. SparySally E. ChapmanJohn K. SinfieldAzhar MaqboolJean KayeTrevor F.C. BattenOestrogen influences autonomic function via actions at classical nuclear oestrogen receptors α and β in the brain, and recent evidence suggests the orphan G protein-coupled receptor GPR30 may also function as a cytoplasmic oestrogen receptor. We investigated the expression of GPR30 in female rat brains throughout the oestrous cycle and after ovariectomy to determine whether GPR30 expression in central autonomic nuclei is correlated with circulating oestrogen levels. In the nucleus of the solitary tract (NTS), ventrolateral medulla (VLM) and periaqueductal gray (PAG) GPR30 mRNA, quantified by real-time PCR, was increased in proestrus and oestrus. In ovariectomised (OVX) rats, expression in NTS and VLM appeared increased compared to metoestrus, but in the hypothalamic paraventricular nucleus and PAG lower mRNA levels were seen in OVX. GPR30-like immunoreactivity (GPR30-LI) colocalised with Golgi in neurones in many brain areas associated with autonomic pathways, and analysis of numbers of immunoreactive neurones showed differences consistent with the PCR data. GPR30-LI was found in a variety of transmitter phenotypes, including cholinergic, serotonergic, catecholaminergic and nitrergic neurones in different neuronal groups. These observations support the view that GPR30 could act as a rapid transducer responding to oestrogen levels and thus modulate the activity of central autonomic pathways.http://www.karger.com/Article/FullText/333296Ventrolateral medullaGPR30HypothalamusPeriaqueductal grayOestrogen receptorNucleus tractus solitariusOestrous cycleOvariectomy |
| spellingShingle | Emma J. Spary Sally E. Chapman John K. Sinfield Azhar Maqbool Jean Kaye Trevor F.C. Batten Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous Cycle Neurosignals Ventrolateral medulla GPR30 Hypothalamus Periaqueductal gray Oestrogen receptor Nucleus tractus solitarius Oestrous cycle Ovariectomy |
| title | Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous Cycle |
| title_full | Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous Cycle |
| title_fullStr | Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous Cycle |
| title_full_unstemmed | Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous Cycle |
| title_short | Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous Cycle |
| title_sort | novel g protein coupled oestrogen receptor gpr30 shows changes in mrna expression in the rat brain over the oestrous cycle |
| topic | Ventrolateral medulla GPR30 Hypothalamus Periaqueductal gray Oestrogen receptor Nucleus tractus solitarius Oestrous cycle Ovariectomy |
| url | http://www.karger.com/Article/FullText/333296 |
| work_keys_str_mv | AT emmajspary novelgproteincoupledoestrogenreceptorgpr30showschangesinmrnaexpressionintheratbrainovertheoestrouscycle AT sallyechapman novelgproteincoupledoestrogenreceptorgpr30showschangesinmrnaexpressionintheratbrainovertheoestrouscycle AT johnksinfield novelgproteincoupledoestrogenreceptorgpr30showschangesinmrnaexpressionintheratbrainovertheoestrouscycle AT azharmaqbool novelgproteincoupledoestrogenreceptorgpr30showschangesinmrnaexpressionintheratbrainovertheoestrouscycle AT jeankaye novelgproteincoupledoestrogenreceptorgpr30showschangesinmrnaexpressionintheratbrainovertheoestrouscycle AT trevorfcbatten novelgproteincoupledoestrogenreceptorgpr30showschangesinmrnaexpressionintheratbrainovertheoestrouscycle |