Recombinant <i>Lactobacillus casei</i> Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits
Rabbit hemorrhagic disease virus (RHDV) is the causative agent of rabbit hemorrhagic disease (RHD). RHD, characterized by hemorrhaging, liver necrosis, and high morbidity and mortality in rabbits and hares, causes severe economic losses in the rabbit industry worldwide. Due to the lack of an efficie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/7/4/172 |
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| author | Li Wang Tian Xia Tiantian Guo Yi Ru Yanping Jiang Wen Cui Han Zhou Xinyuan Qiao Lijie Tang Yigang Xu Yijing Li |
| author_facet | Li Wang Tian Xia Tiantian Guo Yi Ru Yanping Jiang Wen Cui Han Zhou Xinyuan Qiao Lijie Tang Yigang Xu Yijing Li |
| author_sort | Li Wang |
| collection | DOAJ |
| description | Rabbit hemorrhagic disease virus (RHDV) is the causative agent of rabbit hemorrhagic disease (RHD). RHD, characterized by hemorrhaging, liver necrosis, and high morbidity and mortality in rabbits and hares, causes severe economic losses in the rabbit industry worldwide. Due to the lack of an efficient in-vitro propagation system for RHDV, the current vaccine is produced via chemical inactivation of crude RHDV preparation derived from the livers of infected rabbits. Inactivated vaccines are effective for controlling RHD, but the potential problems of biosafety and animal welfare have negative effects on the application of inactivated vaccines. In this study, an oral Lactobacillus casei (<i>L. casei</i>) vaccine was used as an antigen delivery system to express RHDV capsid protein VP60(VP1)-eGFP fusion protein. The expression of the recombinant protein was confirmed via western blotting and immunofluorescence (IFA). Our results indicate that oral administration of this probiotic vaccine can stimulate secretory immunoglobulin A (SIgA)-based mucosal and IgG-based humoral immune responses in rabbits. The immunized rabbits were completely protected against challenge with RHDV. Our findings indicate that the <i>L. casei</i> expression system is a new strategy for the development of a safe and efficient vaccine against RHDV. |
| first_indexed | 2024-04-14T05:18:04Z |
| format | Article |
| id | doaj.art-05d2cfc02aee43b983779b42d437dc12 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-14T05:18:04Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-05d2cfc02aee43b983779b42d437dc122022-12-22T02:10:18ZengMDPI AGVaccines2076-393X2019-11-017417210.3390/vaccines7040172vaccines7040172Recombinant <i>Lactobacillus casei</i> Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in RabbitsLi Wang0Tian Xia1Tiantian Guo2Yi Ru3Yanping Jiang4Wen Cui5Han Zhou6Xinyuan Qiao7Lijie Tang8Yigang Xu9Yijing Li10College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaRabbit hemorrhagic disease virus (RHDV) is the causative agent of rabbit hemorrhagic disease (RHD). RHD, characterized by hemorrhaging, liver necrosis, and high morbidity and mortality in rabbits and hares, causes severe economic losses in the rabbit industry worldwide. Due to the lack of an efficient in-vitro propagation system for RHDV, the current vaccine is produced via chemical inactivation of crude RHDV preparation derived from the livers of infected rabbits. Inactivated vaccines are effective for controlling RHD, but the potential problems of biosafety and animal welfare have negative effects on the application of inactivated vaccines. In this study, an oral Lactobacillus casei (<i>L. casei</i>) vaccine was used as an antigen delivery system to express RHDV capsid protein VP60(VP1)-eGFP fusion protein. The expression of the recombinant protein was confirmed via western blotting and immunofluorescence (IFA). Our results indicate that oral administration of this probiotic vaccine can stimulate secretory immunoglobulin A (SIgA)-based mucosal and IgG-based humoral immune responses in rabbits. The immunized rabbits were completely protected against challenge with RHDV. Our findings indicate that the <i>L. casei</i> expression system is a new strategy for the development of a safe and efficient vaccine against RHDV.https://www.mdpi.com/2076-393X/7/4/172<i>lactobacillus casei</i> (<i>l. casei</i>)rabbit hemorrhagic disease virus (rhdv)vaccinevp60(vp1) |
| spellingShingle | Li Wang Tian Xia Tiantian Guo Yi Ru Yanping Jiang Wen Cui Han Zhou Xinyuan Qiao Lijie Tang Yigang Xu Yijing Li Recombinant <i>Lactobacillus casei</i> Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits Vaccines <i>lactobacillus casei</i> (<i>l. casei</i>) rabbit hemorrhagic disease virus (rhdv) vaccine vp60(vp1) |
| title | Recombinant <i>Lactobacillus casei</i> Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits |
| title_full | Recombinant <i>Lactobacillus casei</i> Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits |
| title_fullStr | Recombinant <i>Lactobacillus casei</i> Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits |
| title_full_unstemmed | Recombinant <i>Lactobacillus casei</i> Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits |
| title_short | Recombinant <i>Lactobacillus casei</i> Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits |
| title_sort | recombinant i lactobacillus casei i expressing capsid protein vp60 can serve as vaccine against rabbit hemorrhagic disease virus in rabbits |
| topic | <i>lactobacillus casei</i> (<i>l. casei</i>) rabbit hemorrhagic disease virus (rhdv) vaccine vp60(vp1) |
| url | https://www.mdpi.com/2076-393X/7/4/172 |
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