Transcript Profiles of Blumeria graminis Development During Infection Reveal a Cluster of Genes That Are Potential Virulence Determinants
High-density cDNA microarrays (2,027 unigenes) were used to analyze transcript profiles of the plant-pathogenic fungus Blumeria graminis f. sp. hordei throughout its asexual life cycle and development of infection. RNA was obtained from four stages preceding penetration and four stages after penetra...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2005-02-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0125 |
| _version_ | 1811264428465717248 |
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| author | Maike Both Sabine E. Eckert Michael Csukai Elisabeth Müller George Dimopoulos Pietro D. Spanu |
| author_facet | Maike Both Sabine E. Eckert Michael Csukai Elisabeth Müller George Dimopoulos Pietro D. Spanu |
| author_sort | Maike Both |
| collection | DOAJ |
| description | High-density cDNA microarrays (2,027 unigenes) were used to analyze transcript profiles of the plant-pathogenic fungus Blumeria graminis f. sp. hordei throughout its asexual life cycle and development of infection. RNA was obtained from four stages preceding penetration and four stages after penetration of the host cells. The microarray data was validated by comparing the expression of a plasma membrane H+-ATPase and fructose-1,6-bis phosphatase with the data obtained from a quantitative polymerase chain reaction (PCR) assay. The results showed that there was a global switch in expression between the pre- and postpenetrative stages. This was largely due to accumulation of RNA encoding protein biosynthesis genes in the late stages. Other functional clusters, such as virulence-related genes and sterol metabolism genes, are up-regulated in pre- and postpenetration stages, respectively. A group of RNAs whose abundance correlated with the expression of cap20, a gene known to be required for virulence in Colletotrichum gloeosporioides, identified genes that are strong candidates for pathogenicity factors in B. graminis. |
| first_indexed | 2024-04-12T20:04:08Z |
| format | Article |
| id | doaj.art-05d4a189868d4691a5932bac265f8554 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:04:08Z |
| publishDate | 2005-02-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-05d4a189868d4691a5932bac265f85542022-12-22T03:18:26ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062005-02-0118212513310.1094/MPMI-18-0125Transcript Profiles of Blumeria graminis Development During Infection Reveal a Cluster of Genes That Are Potential Virulence DeterminantsMaike BothSabine E. EckertMichael CsukaiElisabeth MüllerGeorge DimopoulosPietro D. SpanuHigh-density cDNA microarrays (2,027 unigenes) were used to analyze transcript profiles of the plant-pathogenic fungus Blumeria graminis f. sp. hordei throughout its asexual life cycle and development of infection. RNA was obtained from four stages preceding penetration and four stages after penetration of the host cells. The microarray data was validated by comparing the expression of a plasma membrane H+-ATPase and fructose-1,6-bis phosphatase with the data obtained from a quantitative polymerase chain reaction (PCR) assay. The results showed that there was a global switch in expression between the pre- and postpenetrative stages. This was largely due to accumulation of RNA encoding protein biosynthesis genes in the late stages. Other functional clusters, such as virulence-related genes and sterol metabolism genes, are up-regulated in pre- and postpenetration stages, respectively. A group of RNAs whose abundance correlated with the expression of cap20, a gene known to be required for virulence in Colletotrichum gloeosporioides, identified genes that are strong candidates for pathogenicity factors in B. graminis.https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0125 |
| spellingShingle | Maike Both Sabine E. Eckert Michael Csukai Elisabeth Müller George Dimopoulos Pietro D. Spanu Transcript Profiles of Blumeria graminis Development During Infection Reveal a Cluster of Genes That Are Potential Virulence Determinants Molecular Plant-Microbe Interactions |
| title | Transcript Profiles of Blumeria graminis Development During Infection Reveal a Cluster of Genes That Are Potential Virulence Determinants |
| title_full | Transcript Profiles of Blumeria graminis Development During Infection Reveal a Cluster of Genes That Are Potential Virulence Determinants |
| title_fullStr | Transcript Profiles of Blumeria graminis Development During Infection Reveal a Cluster of Genes That Are Potential Virulence Determinants |
| title_full_unstemmed | Transcript Profiles of Blumeria graminis Development During Infection Reveal a Cluster of Genes That Are Potential Virulence Determinants |
| title_short | Transcript Profiles of Blumeria graminis Development During Infection Reveal a Cluster of Genes That Are Potential Virulence Determinants |
| title_sort | transcript profiles of blumeria graminis development during infection reveal a cluster of genes that are potential virulence determinants |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0125 |
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