Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex
The nuclear pore complex (NPC) facilitates the trafficking of proteins and RNA between the nucleus and cytoplasm. The role of nucleoporins (Nups) in transport in the context of the NPC is well established, yet their function in tRNA export has not been fully explored. We selected several nucleoporin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | RNA Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15476286.2022.2067711 |
| _version_ | 1797403946395893760 |
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| author | Eva Hegedűsová Veronika Maršalová Sneha Kulkarni Zdeněk Paris |
| author_facet | Eva Hegedűsová Veronika Maršalová Sneha Kulkarni Zdeněk Paris |
| author_sort | Eva Hegedűsová |
| collection | DOAJ |
| description | The nuclear pore complex (NPC) facilitates the trafficking of proteins and RNA between the nucleus and cytoplasm. The role of nucleoporins (Nups) in transport in the context of the NPC is well established, yet their function in tRNA export has not been fully explored. We selected several nucleoporins from different parts of the NPC to investigate their potential role in tRNA trafficking in Trypanosoma brucei. We show that while all of the nucleoporins studied are essential for cell viability, only TbNup62 and TbNup53a function in tRNA export. In contrast to homologs in yeast TbNup144 and TbNup158, which are part of the inner and outer ring of the NPC, have no role in nuclear tRNA trafficking. Instead, TbNup144 plays a critical role in nuclear division, highlighting the role of nucleoporins beyond nucleocytoplasmic transport. These results suggest that the location of nucleoporins within the NPC is crucial to maintaining various cellular processes. |
| first_indexed | 2024-03-09T02:46:56Z |
| format | Article |
| id | doaj.art-05d4eea07e91443ebbed12311667a159 |
| institution | Directory Open Access Journal |
| issn | 1547-6286 1555-8584 |
| language | English |
| last_indexed | 2024-03-09T02:46:56Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | RNA Biology |
| spelling | doaj.art-05d4eea07e91443ebbed12311667a1592023-12-05T16:09:51ZengTaylor & Francis GroupRNA Biology1547-62861555-85842022-12-0119165066110.1080/15476286.2022.20677112067711Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complexEva Hegedűsová0Veronika Maršalová1Sneha Kulkarni2Zdeněk Paris3Institute of Parasitology, Biology Centre, Czech Academy of SciencesInstitute of Parasitology, Biology Centre, Czech Academy of SciencesInstitute of Parasitology, Biology Centre, Czech Academy of SciencesInstitute of Parasitology, Biology Centre, Czech Academy of SciencesThe nuclear pore complex (NPC) facilitates the trafficking of proteins and RNA between the nucleus and cytoplasm. The role of nucleoporins (Nups) in transport in the context of the NPC is well established, yet their function in tRNA export has not been fully explored. We selected several nucleoporins from different parts of the NPC to investigate their potential role in tRNA trafficking in Trypanosoma brucei. We show that while all of the nucleoporins studied are essential for cell viability, only TbNup62 and TbNup53a function in tRNA export. In contrast to homologs in yeast TbNup144 and TbNup158, which are part of the inner and outer ring of the NPC, have no role in nuclear tRNA trafficking. Instead, TbNup144 plays a critical role in nuclear division, highlighting the role of nucleoporins beyond nucleocytoplasmic transport. These results suggest that the location of nucleoporins within the NPC is crucial to maintaining various cellular processes.http://dx.doi.org/10.1080/15476286.2022.2067711trna traffickingnucleoporinsnuclear divisionfg-nupsnpctrypanosoma brucei |
| spellingShingle | Eva Hegedűsová Veronika Maršalová Sneha Kulkarni Zdeněk Paris Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex RNA Biology trna trafficking nucleoporins nuclear division fg-nups npc trypanosoma brucei |
| title | Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex |
| title_full | Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex |
| title_fullStr | Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex |
| title_full_unstemmed | Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex |
| title_short | Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex |
| title_sort | trafficking and or division distinct roles of nucleoporins based on their location within the nuclear pore complex |
| topic | trna trafficking nucleoporins nuclear division fg-nups npc trypanosoma brucei |
| url | http://dx.doi.org/10.1080/15476286.2022.2067711 |
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