Molecular Network-Guided Alkaloid Profiling of Aerial Parts of <i>Papaver nudicaule</i> L. Using LC-HRMS
<i>Papaver nudicaule</i> L. (Iceland poppy) is widely used for ornamental purposes. A previous study demonstrated the alleviation of lipopolysaccharide-induced inflammation mediated by <i>P. nudicaule</i> extract through nuclear factor-kappa B and signal transducer and activa...
| প্রধান লেখক: | , , , , |
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| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
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MDPI AG
2020-06-01
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| মালা: | Molecules |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://www.mdpi.com/1420-3049/25/11/2636 |
| _version_ | 1827715543825645568 |
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| author | Kwangho Song Jae-Hyeon Oh Min Young Lee Seok-Geun Lee In Jin Ha |
| author_facet | Kwangho Song Jae-Hyeon Oh Min Young Lee Seok-Geun Lee In Jin Ha |
| author_sort | Kwangho Song |
| collection | DOAJ |
| description | <i>Papaver nudicaule</i> L. (Iceland poppy) is widely used for ornamental purposes. A previous study demonstrated the alleviation of lipopolysaccharide-induced inflammation mediated by <i>P. nudicaule</i> extract through nuclear factor-kappa B and signal transducer and activator of transcription 3 inactivation. As isoquinoline alkaloids are chemical markers and bioactive constituents of <i>Papaver</i> species, the present study investigated the alkaloid profile of aerial parts of five <i>P. nudicaule</i> cultivars with different flower colors and a <i>P. rhoeas</i> cropped for two years. A combination of liquid chromatography high-resolution mass spectrometry and molecular networking was used to cluster isoquinoline alkaloids in the species and highlight the possible metabolites. Aside from the 12 compounds, including rotundine, muramine, and allocryptopine, identified from Global Natural Products Social library and reported information, 46 structurally related metabolites were quantitatively investigated. Forty-two and 16 compounds were proposed for chemical profiles of <i>P. nudicaule</i> and <i>P. rhoeas</i>, respectively. Some species-specific metabolites showed similar fragmentation patterns. The alkaloid abundance of <i>P. nudicaule</i> differed depending on the flower color, and the possible chemical markers were proposed. These results show that molecular networking-guided dereplication allows investigation of unidentified metabolites. The derived chemical profile may facilitate evaluation of <i>P. nudicaule</i> quality for pharmacological applications. |
| first_indexed | 2024-03-10T19:20:28Z |
| format | Article |
| id | doaj.art-05d5b7fe0352406d9a3ba3a8a48d0326 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:20:28Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-05d5b7fe0352406d9a3ba3a8a48d03262023-11-20T02:58:46ZengMDPI AGMolecules1420-30492020-06-012511263610.3390/molecules25112636Molecular Network-Guided Alkaloid Profiling of Aerial Parts of <i>Papaver nudicaule</i> L. Using LC-HRMSKwangho Song0Jae-Hyeon Oh1Min Young Lee2Seok-Geun Lee3In Jin Ha4Korean Medicine Clinical Trial Center (K-CTC), Kyung Hee University Korean Medicine Hospital, Seoul 02454, KoreaGene Engineering Division, National institute of Agricultural Sciences, Rural Development administration, Jeonju-si, Jeollabuk-do 54874, KoreaKorean Medicine Clinical Trial Center (K-CTC), Kyung Hee University Korean Medicine Hospital, Seoul 02454, KoreaKorean Medicine Clinical Trial Center (K-CTC), Kyung Hee University Korean Medicine Hospital, Seoul 02454, KoreaKorean Medicine Clinical Trial Center (K-CTC), Kyung Hee University Korean Medicine Hospital, Seoul 02454, Korea<i>Papaver nudicaule</i> L. (Iceland poppy) is widely used for ornamental purposes. A previous study demonstrated the alleviation of lipopolysaccharide-induced inflammation mediated by <i>P. nudicaule</i> extract through nuclear factor-kappa B and signal transducer and activator of transcription 3 inactivation. As isoquinoline alkaloids are chemical markers and bioactive constituents of <i>Papaver</i> species, the present study investigated the alkaloid profile of aerial parts of five <i>P. nudicaule</i> cultivars with different flower colors and a <i>P. rhoeas</i> cropped for two years. A combination of liquid chromatography high-resolution mass spectrometry and molecular networking was used to cluster isoquinoline alkaloids in the species and highlight the possible metabolites. Aside from the 12 compounds, including rotundine, muramine, and allocryptopine, identified from Global Natural Products Social library and reported information, 46 structurally related metabolites were quantitatively investigated. Forty-two and 16 compounds were proposed for chemical profiles of <i>P. nudicaule</i> and <i>P. rhoeas</i>, respectively. Some species-specific metabolites showed similar fragmentation patterns. The alkaloid abundance of <i>P. nudicaule</i> differed depending on the flower color, and the possible chemical markers were proposed. These results show that molecular networking-guided dereplication allows investigation of unidentified metabolites. The derived chemical profile may facilitate evaluation of <i>P. nudicaule</i> quality for pharmacological applications.https://www.mdpi.com/1420-3049/25/11/2636<i>Papaver nudicaule</i>alkaloidsLC-MS/MS chemical profilingGNPS molecular networking |
| spellingShingle | Kwangho Song Jae-Hyeon Oh Min Young Lee Seok-Geun Lee In Jin Ha Molecular Network-Guided Alkaloid Profiling of Aerial Parts of <i>Papaver nudicaule</i> L. Using LC-HRMS Molecules <i>Papaver nudicaule</i> alkaloids LC-MS/MS chemical profiling GNPS molecular networking |
| title | Molecular Network-Guided Alkaloid Profiling of Aerial Parts of <i>Papaver nudicaule</i> L. Using LC-HRMS |
| title_full | Molecular Network-Guided Alkaloid Profiling of Aerial Parts of <i>Papaver nudicaule</i> L. Using LC-HRMS |
| title_fullStr | Molecular Network-Guided Alkaloid Profiling of Aerial Parts of <i>Papaver nudicaule</i> L. Using LC-HRMS |
| title_full_unstemmed | Molecular Network-Guided Alkaloid Profiling of Aerial Parts of <i>Papaver nudicaule</i> L. Using LC-HRMS |
| title_short | Molecular Network-Guided Alkaloid Profiling of Aerial Parts of <i>Papaver nudicaule</i> L. Using LC-HRMS |
| title_sort | molecular network guided alkaloid profiling of aerial parts of i papaver nudicaule i l using lc hrms |
| topic | <i>Papaver nudicaule</i> alkaloids LC-MS/MS chemical profiling GNPS molecular networking |
| url | https://www.mdpi.com/1420-3049/25/11/2636 |
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