miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy

GRIN2A is associated with epilepsy (EP); however, its regulatory mechanism involving upstream miRNA (miR-30b-5p) has been overlooked. In this study, we aimed to identify the regulatory mechanism of the miR-30b-5p/GRIN2A axis in EP. Hippocampal neurons isolated from mice were incubated in magnesium-f...

Full description

Bibliographic Details
Main Authors: Zheng Hu, Wu Liuyang, Yuan Huisheng
Format: Article
Language:English
Published: De Gruyter 2023-03-01
Series:Open Medicine
Subjects:
Online Access:https://doi.org/10.1515/med-2023-0675
_version_ 1797848617920233472
author Zheng Hu
Wu Liuyang
Yuan Huisheng
author_facet Zheng Hu
Wu Liuyang
Yuan Huisheng
author_sort Zheng Hu
collection DOAJ
description GRIN2A is associated with epilepsy (EP); however, its regulatory mechanism involving upstream miRNA (miR-30b-5p) has been overlooked. In this study, we aimed to identify the regulatory mechanism of the miR-30b-5p/GRIN2A axis in EP. Hippocampal neurons isolated from mice were incubated in magnesium-free medium for 48 h to establish an in vitro EP model. An in vivo model of EP was constructed by the intraperitoneal injection of atropine into mice. Nissl staining and hematoxylin and eosin staining were used to evaluate pathological injuries in the hippocampal CA1 regions of mice. The CCK8 assay confirmed that miR-30b-5p overexpression restored the suppressed proliferative capacity of hippocampal neurons exposed to magnesium-free conditions. Caspase-3 activity assay revealed that miR-30b-5p overexpression abrogated the increased apoptosis of hippocampal neurons under magnesium-free conditions. In an in vivo model of EP, miR-30b-5p overexpression reversed pathological injuries in the hippocampal CA1 regions of mice and abrogated the increased apoptosis in the EP mouse model. Luciferase assays and western blotting confirmed that miR-30b-5p targeted GRIN2A, thereby inhibiting GRIN2A expression. Overall, miR-30b-5p can protect against cell proliferation and attenuate apoptosis in hippocampal neurons under magnesium-free conditions by targeting GRIN2A.
first_indexed 2024-04-09T18:31:31Z
format Article
id doaj.art-05d6fcfaef534edb9e5ec708df3cd264
institution Directory Open Access Journal
issn 2391-5463
language English
last_indexed 2024-04-09T18:31:31Z
publishDate 2023-03-01
publisher De Gruyter
record_format Article
series Open Medicine
spelling doaj.art-05d6fcfaef534edb9e5ec708df3cd2642023-04-11T17:07:17ZengDe GruyterOpen Medicine2391-54632023-03-0118118112410.1515/med-2023-0675miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsyZheng Hu0Wu Liuyang1Yuan Huisheng2Department of Neurosurgery, Hubei Provincial Hospital of Integrated Chinese & Western Medicine, Wuhan430015, Hubei, ChinaDepartment of Neurosurgery, Hubei Provincial Hospital of Integrated Chinese & Western Medicine, Wuhan430015, Hubei, ChinaDepartment of Neurosurgery, Hubei Provincial Hospital of Integrated Chinese & Western Medicine, No. 11 Lingjiaohu Road, Jianghan District, Wuhan430015, Hubei, ChinaGRIN2A is associated with epilepsy (EP); however, its regulatory mechanism involving upstream miRNA (miR-30b-5p) has been overlooked. In this study, we aimed to identify the regulatory mechanism of the miR-30b-5p/GRIN2A axis in EP. Hippocampal neurons isolated from mice were incubated in magnesium-free medium for 48 h to establish an in vitro EP model. An in vivo model of EP was constructed by the intraperitoneal injection of atropine into mice. Nissl staining and hematoxylin and eosin staining were used to evaluate pathological injuries in the hippocampal CA1 regions of mice. The CCK8 assay confirmed that miR-30b-5p overexpression restored the suppressed proliferative capacity of hippocampal neurons exposed to magnesium-free conditions. Caspase-3 activity assay revealed that miR-30b-5p overexpression abrogated the increased apoptosis of hippocampal neurons under magnesium-free conditions. In an in vivo model of EP, miR-30b-5p overexpression reversed pathological injuries in the hippocampal CA1 regions of mice and abrogated the increased apoptosis in the EP mouse model. Luciferase assays and western blotting confirmed that miR-30b-5p targeted GRIN2A, thereby inhibiting GRIN2A expression. Overall, miR-30b-5p can protect against cell proliferation and attenuate apoptosis in hippocampal neurons under magnesium-free conditions by targeting GRIN2A.https://doi.org/10.1515/med-2023-0675epilepsymirnatargetingproliferation
spellingShingle Zheng Hu
Wu Liuyang
Yuan Huisheng
miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy
Open Medicine
epilepsy
mirna
targeting
proliferation
title miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy
title_full miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy
title_fullStr miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy
title_full_unstemmed miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy
title_short miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy
title_sort mir 30b 5p targeting grin2a inhibits hippocampal damage in epilepsy
topic epilepsy
mirna
targeting
proliferation
url https://doi.org/10.1515/med-2023-0675
work_keys_str_mv AT zhenghu mir30b5ptargetinggrin2ainhibitshippocampaldamageinepilepsy
AT wuliuyang mir30b5ptargetinggrin2ainhibitshippocampaldamageinepilepsy
AT yuanhuisheng mir30b5ptargetinggrin2ainhibitshippocampaldamageinepilepsy