Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidate
Background and Objectives: Nontypeable Haemophilus influenzae (NTHi) are major causes of non-invasive infections, including otitis media and sinusitis and it can also contribute to respiratory infections of all ages. Currently, there is no licensed vaccine against NTHi commercially available. Many s...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-10-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/2049 |
| _version_ | 1818384104756871168 |
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| author | Ava Behrouzi Reza Arabi Mianroodi Parviz Afrough Ahmad Ayadi Amirarsalan Serajian |
| author_facet | Ava Behrouzi Reza Arabi Mianroodi Parviz Afrough Ahmad Ayadi Amirarsalan Serajian |
| author_sort | Ava Behrouzi |
| collection | DOAJ |
| description | Background and Objectives: Nontypeable Haemophilus influenzae (NTHi) are major causes of non-invasive infections, including otitis media and sinusitis and it can also contribute to respiratory infections of all ages. Currently, there is no licensed vaccine against NTHi commercially available. Many studies have been conducted on the use of OMV as a vaccine against NTHi. The purpose of this study is to achieve an immunogenic vaccine against NTHi.
Materials and Methods: In this study, standard OMV Haemophilus (ATCC49766) with adjuncts CpG and MPLA was used and after infusion into BALB/c mice, the levels of antibodies and cytokines were measured on serum of immunized mice.
Results: The results showed that total IgG antibody and IgG1 and IgG2a isotypes in OMV immunized mice with mixture of CpG-MPLA adjuvant had a significant increase. Also, the results of cytokines (IL-10, IL-4 and IFN-γ) showed that IL-4 had the highest rate.
Conclusion: These findings indicate that OMVs derived from NTHi strains have a high potential to act as a vaccine against NTHi infections. |
| first_indexed | 2024-12-14T03:16:58Z |
| format | Article |
| id | doaj.art-05d7fe3888b149b8b449b777643588d5 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-14T03:16:58Z |
| publishDate | 2020-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-05d7fe3888b149b8b449b777643588d52022-12-21T23:19:06ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472020-10-0112510.18502/ijm.v12i5.4602Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidateAva Behrouzi0Reza Arabi Mianroodi1Parviz Afrough2Ahmad Ayadi3Amirarsalan Serajian4Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranDepartment of R&D, Research and Production Complex, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranDepartment of Health Education, ACECR, Khuzestan, Ahvaz, IranBackground and Objectives: Nontypeable Haemophilus influenzae (NTHi) are major causes of non-invasive infections, including otitis media and sinusitis and it can also contribute to respiratory infections of all ages. Currently, there is no licensed vaccine against NTHi commercially available. Many studies have been conducted on the use of OMV as a vaccine against NTHi. The purpose of this study is to achieve an immunogenic vaccine against NTHi. Materials and Methods: In this study, standard OMV Haemophilus (ATCC49766) with adjuncts CpG and MPLA was used and after infusion into BALB/c mice, the levels of antibodies and cytokines were measured on serum of immunized mice. Results: The results showed that total IgG antibody and IgG1 and IgG2a isotypes in OMV immunized mice with mixture of CpG-MPLA adjuvant had a significant increase. Also, the results of cytokines (IL-10, IL-4 and IFN-γ) showed that IL-4 had the highest rate. Conclusion: These findings indicate that OMVs derived from NTHi strains have a high potential to act as a vaccine against NTHi infections.https://ijm.tums.ac.ir/index.php/ijm/article/view/2049Non typeable Haemophilus influenza;Outer membrane vesicle;Vaccine |
| spellingShingle | Ava Behrouzi Reza Arabi Mianroodi Parviz Afrough Ahmad Ayadi Amirarsalan Serajian Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidate Iranian Journal of Microbiology Non typeable Haemophilus influenza; Outer membrane vesicle; Vaccine |
| title | Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidate |
| title_full | Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidate |
| title_fullStr | Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidate |
| title_full_unstemmed | Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidate |
| title_short | Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidate |
| title_sort | evaluation of immunological responses against outer membrane vesicles omv of nontypeable haemophilus influenzae using mpla cpg adjuvant as a vaccine candidate |
| topic | Non typeable Haemophilus influenza; Outer membrane vesicle; Vaccine |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/2049 |
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