Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere
Abstract Plastic accumulation is a severe threat to the environment due to its resistivity to thermal, mechanical and biological processes. In recent years, microbial degradation of plastic waste disposal is of interest because of its eco-friendly nature. In this study, a total of 33 fungi were isol...
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-59032-4 |
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| author | M. Sathiyabama R. V. Boomija T. Sathiyamoorthy N. Mathivanan R. Balaji |
| author_facet | M. Sathiyabama R. V. Boomija T. Sathiyamoorthy N. Mathivanan R. Balaji |
| author_sort | M. Sathiyabama |
| collection | DOAJ |
| description | Abstract Plastic accumulation is a severe threat to the environment due to its resistivity to thermal, mechanical and biological processes. In recent years, microbial degradation of plastic waste disposal is of interest because of its eco-friendly nature. In this study, a total of 33 fungi were isolated from the plastisphere and out of which 28 fungal species showed halo zone of clearance in agarized LDPE media. The fungus showing highest zone of clearance was further used to evaluate its degradation potential. Based on morphological and molecular technique, the fungus was identified as Cladosporium sphaerospermum. The biodegradation of LDPE by C. sphaerospermum was evaluated by various methods. The exposure of LDPE with C. sphaerospermum resulted in weight loss (15.23%) in seven days, higher reduction rate (0.0224/day) and lower half-life (30.93 days). FTIR analysis showed changes in functional group and increased carbonyl index in LDPE treated with C. sphaerospermum. SEMimages evidenced the formation of pits, surface aberrations and grooves on the LDPE film treated with the fungus whereas the untreated control LDPE film showed no change. AFM analysis confirmed the surface changes and roughness in fungus treated LDPE film. This might be due to the extracellular lignolytic enzymes secreted by C. sphaerospermum grown on LDPE. The degradation of polyethylene by Short chain alkanes such as dodecane, hexasiloxane and silane were identified in the extract of fungus incubated with LDPE film through GC–MS analysis which might be due to the degradation of LDPE film by C. sphaerospermum. This was the first report on the LDPE degradation by C. sphaerospermum in very short duration which enables green scavenging of plastic wastes. |
| first_indexed | 2024-04-24T09:53:53Z |
| format | Article |
| id | doaj.art-05da2ab161e7491c89d07b4f27240b32 |
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| language | English |
| last_indexed | 2024-04-24T09:53:53Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-05da2ab161e7491c89d07b4f27240b322024-04-14T11:15:01ZengNature PortfolioScientific Reports2045-23222024-04-0114111310.1038/s41598-024-59032-4Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphereM. Sathiyabama0R. V. Boomija1T. Sathiyamoorthy2N. Mathivanan3R. Balaji4Department of Botany, School of Life Sciences, Bharathidasan UniversityDepartment of Botany, School of Life Sciences, Bharathidasan UniversityCAS in Botany, University of MadrasCAS in Botany, University of MadrasCAS in Botany, University of MadrasAbstract Plastic accumulation is a severe threat to the environment due to its resistivity to thermal, mechanical and biological processes. In recent years, microbial degradation of plastic waste disposal is of interest because of its eco-friendly nature. In this study, a total of 33 fungi were isolated from the plastisphere and out of which 28 fungal species showed halo zone of clearance in agarized LDPE media. The fungus showing highest zone of clearance was further used to evaluate its degradation potential. Based on morphological and molecular technique, the fungus was identified as Cladosporium sphaerospermum. The biodegradation of LDPE by C. sphaerospermum was evaluated by various methods. The exposure of LDPE with C. sphaerospermum resulted in weight loss (15.23%) in seven days, higher reduction rate (0.0224/day) and lower half-life (30.93 days). FTIR analysis showed changes in functional group and increased carbonyl index in LDPE treated with C. sphaerospermum. SEMimages evidenced the formation of pits, surface aberrations and grooves on the LDPE film treated with the fungus whereas the untreated control LDPE film showed no change. AFM analysis confirmed the surface changes and roughness in fungus treated LDPE film. This might be due to the extracellular lignolytic enzymes secreted by C. sphaerospermum grown on LDPE. The degradation of polyethylene by Short chain alkanes such as dodecane, hexasiloxane and silane were identified in the extract of fungus incubated with LDPE film through GC–MS analysis which might be due to the degradation of LDPE film by C. sphaerospermum. This was the first report on the LDPE degradation by C. sphaerospermum in very short duration which enables green scavenging of plastic wastes.https://doi.org/10.1038/s41598-024-59032-4MycodegradationLDPEWeight lossSurface erosionCladosporium sphaerospermum |
| spellingShingle | M. Sathiyabama R. V. Boomija T. Sathiyamoorthy N. Mathivanan R. Balaji Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere Scientific Reports Mycodegradation LDPE Weight loss Surface erosion Cladosporium sphaerospermum |
| title | Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere |
| title_full | Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere |
| title_fullStr | Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere |
| title_full_unstemmed | Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere |
| title_short | Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere |
| title_sort | mycodegradation of low density polyethylene by cladosporium sphaerospermum isolated from platisphere |
| topic | Mycodegradation LDPE Weight loss Surface erosion Cladosporium sphaerospermum |
| url | https://doi.org/10.1038/s41598-024-59032-4 |
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