A role for lipoprotein lipase during synaptic remodeling in the adult mouse brain
Lipoprotein lipase (LPL) is a member of a lipase family known to hydrolyze triglyceride molecules found in lipoprotein particles. This particular lipase also has a role in the binding of lipoprotein particles to different cell-surface receptors. LPL has been identified in the brain but has no specif...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2004-04-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996103002663 |
| _version_ | 1819163517531455488 |
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| author | Jean-François Blain Eric Paradis Sophie B Gaudreault Danielle Champagne Denis Richard Judes Poirier |
| author_facet | Jean-François Blain Eric Paradis Sophie B Gaudreault Danielle Champagne Denis Richard Judes Poirier |
| author_sort | Jean-François Blain |
| collection | DOAJ |
| description | Lipoprotein lipase (LPL) is a member of a lipase family known to hydrolyze triglyceride molecules found in lipoprotein particles. This particular lipase also has a role in the binding of lipoprotein particles to different cell-surface receptors. LPL has been identified in the brain but has no specific function yet. This study aimed at elucidating the role of LPL in the brain in response to injury. Mice were subjected to hippocampal deafferentation using the entorhinal cortex lesion and mRNA and protein expression were assessed over a time-course of degeneration/reinnervation. Hippocampal LPL levels peaked at 2 days post-lesion (DPL) both at the mRNA and protein levels. No change was observed for receptors of the LDL-receptor family or RAP at DPL 2 in the hippocampus but the glia-specific syndecan-4 was found to be significantly upregulated at DPL 2. These results suggest that LPL is involved in the recycling of cholesterol and lipids released from degenerating terminals after a lesion through a syndecan-4-dependent pathway. |
| first_indexed | 2024-12-22T17:45:23Z |
| format | Article |
| id | doaj.art-05da37dde1ef4ebd8133c8a190200338 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-22T17:45:23Z |
| publishDate | 2004-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-05da37dde1ef4ebd8133c8a1902003382022-12-21T18:18:19ZengElsevierNeurobiology of Disease1095-953X2004-04-01153510519A role for lipoprotein lipase during synaptic remodeling in the adult mouse brainJean-François Blain0Eric Paradis1Sophie B Gaudreault2Danielle Champagne3Denis Richard4Judes Poirier5Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada H4A 2B4; Douglas Hospital Research Center, Verdun, Quebec, Canada H4H 1R3; Department of Anatomy and Physiology, Faculty of Medicine, Laval University, Ste-Foy, Quebec, Canada; McGill Center for Studies in Aging, Verdun, Quebec, Canada H4H 1R3Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada H4A 2B4; Douglas Hospital Research Center, Verdun, Quebec, Canada H4H 1R3; Department of Anatomy and Physiology, Faculty of Medicine, Laval University, Ste-Foy, Quebec, Canada; McGill Center for Studies in Aging, Verdun, Quebec, Canada H4H 1R3Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada H4A 2B4; Douglas Hospital Research Center, Verdun, Quebec, Canada H4H 1R3; Department of Anatomy and Physiology, Faculty of Medicine, Laval University, Ste-Foy, Quebec, Canada; McGill Center for Studies in Aging, Verdun, Quebec, Canada H4H 1R3Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada H4A 2B4; Douglas Hospital Research Center, Verdun, Quebec, Canada H4H 1R3; Department of Anatomy and Physiology, Faculty of Medicine, Laval University, Ste-Foy, Quebec, Canada; McGill Center for Studies in Aging, Verdun, Quebec, Canada H4H 1R3Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada H4A 2B4; Douglas Hospital Research Center, Verdun, Quebec, Canada H4H 1R3; Department of Anatomy and Physiology, Faculty of Medicine, Laval University, Ste-Foy, Quebec, Canada; McGill Center for Studies in Aging, Verdun, Quebec, Canada H4H 1R3Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada H4A 2B4; Douglas Hospital Research Center, Verdun, Quebec, Canada H4H 1R3; Department of Anatomy and Physiology, Faculty of Medicine, Laval University, Ste-Foy, Quebec, Canada; McGill Center for Studies in Aging, Verdun, Quebec, Canada H4H 1R3Lipoprotein lipase (LPL) is a member of a lipase family known to hydrolyze triglyceride molecules found in lipoprotein particles. This particular lipase also has a role in the binding of lipoprotein particles to different cell-surface receptors. LPL has been identified in the brain but has no specific function yet. This study aimed at elucidating the role of LPL in the brain in response to injury. Mice were subjected to hippocampal deafferentation using the entorhinal cortex lesion and mRNA and protein expression were assessed over a time-course of degeneration/reinnervation. Hippocampal LPL levels peaked at 2 days post-lesion (DPL) both at the mRNA and protein levels. No change was observed for receptors of the LDL-receptor family or RAP at DPL 2 in the hippocampus but the glia-specific syndecan-4 was found to be significantly upregulated at DPL 2. These results suggest that LPL is involved in the recycling of cholesterol and lipids released from degenerating terminals after a lesion through a syndecan-4-dependent pathway.http://www.sciencedirect.com/science/article/pii/S0969996103002663Lipoprotein lipaseCholesterolRecyclingApolipoprotein EEntorhinal cortex lesionHippocampus |
| spellingShingle | Jean-François Blain Eric Paradis Sophie B Gaudreault Danielle Champagne Denis Richard Judes Poirier A role for lipoprotein lipase during synaptic remodeling in the adult mouse brain Neurobiology of Disease Lipoprotein lipase Cholesterol Recycling Apolipoprotein E Entorhinal cortex lesion Hippocampus |
| title | A role for lipoprotein lipase during synaptic remodeling in the adult mouse brain |
| title_full | A role for lipoprotein lipase during synaptic remodeling in the adult mouse brain |
| title_fullStr | A role for lipoprotein lipase during synaptic remodeling in the adult mouse brain |
| title_full_unstemmed | A role for lipoprotein lipase during synaptic remodeling in the adult mouse brain |
| title_short | A role for lipoprotein lipase during synaptic remodeling in the adult mouse brain |
| title_sort | role for lipoprotein lipase during synaptic remodeling in the adult mouse brain |
| topic | Lipoprotein lipase Cholesterol Recycling Apolipoprotein E Entorhinal cortex lesion Hippocampus |
| url | http://www.sciencedirect.com/science/article/pii/S0969996103002663 |
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