Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island
Sponges, a group of marine multicellular animals with a porous body structure, show potential for the production of bioactive compounds. Sponge‐associated bacteria are an alternative antimicrobial producer due to their high content of bioactive compounds. This study aimed to identify the highest‐pot...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2022-09-01
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| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/65943 |
| _version_ | 1811170979481649152 |
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| author | Sipriyadi Sipriyadi Riziq Ilham Nurfahmi Uci Cahlia Risky Hadi Wibowo Welly Darwis Enny Nugraheni |
| author_facet | Sipriyadi Sipriyadi Riziq Ilham Nurfahmi Uci Cahlia Risky Hadi Wibowo Welly Darwis Enny Nugraheni |
| author_sort | Sipriyadi Sipriyadi |
| collection | DOAJ |
| description | Sponges, a group of marine multicellular animals with a porous body structure, show potential for the production of bioactive compounds. Sponge‐associated bacteria are an alternative antimicrobial producer due to their high content of bioactive compounds. This study aimed to identify the highest‐potential antimicrobial‐producing bacteria isolate associated with Jaspis sp. sponges from Enggano island. The isolated bacteria were screened for antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans using cultures, supernatants, pellets, and crude extracts. The study also conducted genetic identification to determine the identity of the isolate with the greatest potency and its closest relationship using the 16S rRNA gene. The antimicrobial activity was determined by monitoring and measuring the diameter of the formed clear zones. The results of the observations of morphological characteristics revealed nine isolates from Jaspis sp. that each consisting of 6 JABS isolates and 3 JABB isolates. Based on isolates that had antimicrobial activity, JABS6 isolates had the best antimicrobial activity, with the diameter of inhibition zones of 24.7, 8.2, 4.6, and 33.7 mm for E. coli, P. aeruginosa, S. aureus and C. albicans, respectively. The genome sequencing of the JABS6 isolate confirmed that it was identical to Bacillus thuringiensis strain USS‐CAP‐1. The study concludes that this finding shows promise for the further development of future antimicrobial agents. |
| first_indexed | 2024-04-10T17:05:49Z |
| format | Article |
| id | doaj.art-05dc4e0349ed4b1a839b757a00b75ba7 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-04-10T17:05:49Z |
| publishDate | 2022-09-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-05dc4e0349ed4b1a839b757a00b75ba72023-02-06T03:00:18ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412022-09-0127316317010.22146/ijbiotech.6594332139Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano IslandSipriyadi Sipriyadi0Riziq Ilham Nurfahmi1Uci Cahlia2Risky Hadi Wibowo3Welly Darwis4Enny Nugraheni5Biology Department, Faculty of Mathematics and Natural Science, Universitas Bengkulu, Jl. W.R Supratman, Kandang Limun, Bengkulu 38125, IndonesiaBiology Department, Faculty of Mathematics and Natural Science, Universitas Bengkulu, Jl. W.R Supratman, Kandang Limun, Bengkulu 38125, IndonesiaBiology Department, Faculty of Mathematics and Natural Science, Universitas Bengkulu, Jl. W.R Supratman, Kandang Limun, Bengkulu 38125, IndonesiaBiology Department, Faculty of Mathematics and Natural Science, Universitas Bengkulu, Jl. W.R Supratman, Kandang Limun, Bengkulu 38125, IndonesiaBiology Department, Faculty of Mathematics and Natural Science, Universitas Bengkulu, Jl. W.R Supratman, Kandang Limun, Bengkulu 38125, IndonesiaMedical Study Program, Faculty of Medicine and Health, Universitas Bengkulu, Jl. W.R Supratman, Kandang Limun, Bengkulu 38125, IndonesiaSponges, a group of marine multicellular animals with a porous body structure, show potential for the production of bioactive compounds. Sponge‐associated bacteria are an alternative antimicrobial producer due to their high content of bioactive compounds. This study aimed to identify the highest‐potential antimicrobial‐producing bacteria isolate associated with Jaspis sp. sponges from Enggano island. The isolated bacteria were screened for antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans using cultures, supernatants, pellets, and crude extracts. The study also conducted genetic identification to determine the identity of the isolate with the greatest potency and its closest relationship using the 16S rRNA gene. The antimicrobial activity was determined by monitoring and measuring the diameter of the formed clear zones. The results of the observations of morphological characteristics revealed nine isolates from Jaspis sp. that each consisting of 6 JABS isolates and 3 JABB isolates. Based on isolates that had antimicrobial activity, JABS6 isolates had the best antimicrobial activity, with the diameter of inhibition zones of 24.7, 8.2, 4.6, and 33.7 mm for E. coli, P. aeruginosa, S. aureus and C. albicans, respectively. The genome sequencing of the JABS6 isolate confirmed that it was identical to Bacillus thuringiensis strain USS‐CAP‐1. The study concludes that this finding shows promise for the further development of future antimicrobial agents.https://jurnal.ugm.ac.id/ijbiotech/article/view/65943antimicrobialbacteriaenggano islandjaspis sp.screening |
| spellingShingle | Sipriyadi Sipriyadi Riziq Ilham Nurfahmi Uci Cahlia Risky Hadi Wibowo Welly Darwis Enny Nugraheni Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island Indonesian Journal of Biotechnology antimicrobial bacteria enggano island jaspis sp. screening |
| title | Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island |
| title_full | Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island |
| title_fullStr | Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island |
| title_full_unstemmed | Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island |
| title_short | Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island |
| title_sort | potential of marine sponge jaspis sp associated bacteria as an antimicrobial producer in enggano island |
| topic | antimicrobial bacteria enggano island jaspis sp. screening |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/65943 |
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