Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract
Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. viola...
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| Format: | Article |
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MDPI AG
2012-03-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/12/4/4016/ |
| _version_ | 1811187049930162176 |
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| author | Kok-Gan Chan Thiba Krishnan Wai-Fong Yin |
| author_facet | Kok-Gan Chan Thiba Krishnan Wai-Fong Yin |
| author_sort | Kok-Gan Chan |
| collection | DOAJ |
| description | Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N‑hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-L-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs. |
| first_indexed | 2024-04-11T13:56:38Z |
| format | Article |
| id | doaj.art-05dcc3ba78844fb695d666eea63313b8 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:56:38Z |
| publishDate | 2012-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-05dcc3ba78844fb695d666eea63313b82022-12-22T04:20:18ZengMDPI AGSensors1424-82202012-03-011244016403010.3390/s120404016Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) Bud ExtractKok-Gan ChanThiba KrishnanWai-Fong YinQuorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N‑hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-L-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs.http://www.mdpi.com/1424-8220/12/4/4016/AyurvedabioluminescencebiosensorsChromobacterium violaceumlecA::luxN-acylhomoserine lactonesPseudomonas aeruginosapyocyaninSyzygium aromaticumswarming |
| spellingShingle | Kok-Gan Chan Thiba Krishnan Wai-Fong Yin Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract Sensors Ayurveda bioluminescence biosensors Chromobacterium violaceum lecA::lux N-acylhomoserine lactones Pseudomonas aeruginosa pyocyanin Syzygium aromaticum swarming |
| title | Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract |
| title_full | Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract |
| title_fullStr | Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract |
| title_full_unstemmed | Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract |
| title_short | Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract |
| title_sort | inhibition of quorum sensing controlled virulence factor production in pseudomonas aeruginosa pao1 by ayurveda spice clove syzygium aromaticum bud extract |
| topic | Ayurveda bioluminescence biosensors Chromobacterium violaceum lecA::lux N-acylhomoserine lactones Pseudomonas aeruginosa pyocyanin Syzygium aromaticum swarming |
| url | http://www.mdpi.com/1424-8220/12/4/4016/ |
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