Molecular characteristics and risk factors associated with linezolid-resistant Enterococcus faecalis infection in Southwest China
Background: Linezolid is one of the last lines of defence for Gram-positive bacteria, and the increasing emergence of linezolid-resistant enterococci (LRE) has become an important problem in nosocomial infections. Objectives: This study aimed to explore the mechanisms of linezolid resistance and ana...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520300916 |
| _version_ | 1823930945102675968 |
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| author | Jiaqi Zou Yun Xia |
| author_facet | Jiaqi Zou Yun Xia |
| author_sort | Jiaqi Zou |
| collection | DOAJ |
| description | Background: Linezolid is one of the last lines of defence for Gram-positive bacteria, and the increasing emergence of linezolid-resistant enterococci (LRE) has become an important problem in nosocomial infections. Objectives: This study aimed to explore the mechanisms of linezolid resistance and analyse risk factors for the infection with LRE. Methods: The known resistance mechanisms of linezolid were detected by polymerase chain reaction (PCR). Clinical data of patients with LRE infection were collected from the electronic medical record system. Risk factors associated with LRE were determined by comparing two case groups (LRE group and linezolid-susceptible enterococci [LSE] group) with the control group (without enterococci infection) at a ratio of 1:1:2. Multivariate logistic regression models were used to evaluate independent risk factors for acquiring LRE and LSE. Results: A total of 85 (4.5%) LRE isolates were detected during a 5-year period. The main resistance mechanism was acquisition of the transferable gene optrA (98.8%), followed by the L4 protein mutation (37.7%) and L3 protein mutation (4.7%). In our study, 85 LRE cases, 85 LSE cases, and 170 controls were then enrolled; multivariable analyses indicated that transfer from another hospital (odds ratio [OR], 3.2; 95% confidence interval [CI], 1.5–7.3; P = 0.004), malignant tumour (OR, 3.7; 95% CI, 1.6–8.8; P = 0.003), polymicrobial infection (OR, 12.2; 95% CI, 1.4–106.2; P = 0.023), and urinary tract infections (OR, 42.7; 95% CI, 11.4–159.3; P = 0.000) were independent predictors for acquiring LRE. In addition, polymicrobial infections (OR, 43.3; 95% CI, 14.1–132.8; P = 0.000), total length of hospital stay (≥30 days) (OR, 5.7; 95% CI, 1.9–17.3; P = 0.002) and exposure to glycopeptide (OR, 6.6; 95% CI, 1.1–38.8; P = 0.035) were independently associated with LSE infections. Conclusions: Proper antibiotic management programs and monitoring of patient risk factors are necessary to control LRE infections. |
| first_indexed | 2024-12-16T21:31:48Z |
| format | Article |
| id | doaj.art-05dce7b7fdb243a695297ce6da872147 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-16T21:31:48Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-05dce7b7fdb243a695297ce6da8721472022-12-21T22:15:32ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-09-0122504510Molecular characteristics and risk factors associated with linezolid-resistant Enterococcus faecalis infection in Southwest ChinaJiaqi Zou0Yun Xia1Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaCorresponding author at: Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, Chongqing 400016, China.; Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaBackground: Linezolid is one of the last lines of defence for Gram-positive bacteria, and the increasing emergence of linezolid-resistant enterococci (LRE) has become an important problem in nosocomial infections. Objectives: This study aimed to explore the mechanisms of linezolid resistance and analyse risk factors for the infection with LRE. Methods: The known resistance mechanisms of linezolid were detected by polymerase chain reaction (PCR). Clinical data of patients with LRE infection were collected from the electronic medical record system. Risk factors associated with LRE were determined by comparing two case groups (LRE group and linezolid-susceptible enterococci [LSE] group) with the control group (without enterococci infection) at a ratio of 1:1:2. Multivariate logistic regression models were used to evaluate independent risk factors for acquiring LRE and LSE. Results: A total of 85 (4.5%) LRE isolates were detected during a 5-year period. The main resistance mechanism was acquisition of the transferable gene optrA (98.8%), followed by the L4 protein mutation (37.7%) and L3 protein mutation (4.7%). In our study, 85 LRE cases, 85 LSE cases, and 170 controls were then enrolled; multivariable analyses indicated that transfer from another hospital (odds ratio [OR], 3.2; 95% confidence interval [CI], 1.5–7.3; P = 0.004), malignant tumour (OR, 3.7; 95% CI, 1.6–8.8; P = 0.003), polymicrobial infection (OR, 12.2; 95% CI, 1.4–106.2; P = 0.023), and urinary tract infections (OR, 42.7; 95% CI, 11.4–159.3; P = 0.000) were independent predictors for acquiring LRE. In addition, polymicrobial infections (OR, 43.3; 95% CI, 14.1–132.8; P = 0.000), total length of hospital stay (≥30 days) (OR, 5.7; 95% CI, 1.9–17.3; P = 0.002) and exposure to glycopeptide (OR, 6.6; 95% CI, 1.1–38.8; P = 0.035) were independently associated with LSE infections. Conclusions: Proper antibiotic management programs and monitoring of patient risk factors are necessary to control LRE infections.http://www.sciencedirect.com/science/article/pii/S2213716520300916Linezolid resistanceEnterococcus faecalisoptrARisk factor |
| spellingShingle | Jiaqi Zou Yun Xia Molecular characteristics and risk factors associated with linezolid-resistant Enterococcus faecalis infection in Southwest China Journal of Global Antimicrobial Resistance Linezolid resistance Enterococcus faecalis optrA Risk factor |
| title | Molecular characteristics and risk factors associated with linezolid-resistant Enterococcus faecalis infection in Southwest China |
| title_full | Molecular characteristics and risk factors associated with linezolid-resistant Enterococcus faecalis infection in Southwest China |
| title_fullStr | Molecular characteristics and risk factors associated with linezolid-resistant Enterococcus faecalis infection in Southwest China |
| title_full_unstemmed | Molecular characteristics and risk factors associated with linezolid-resistant Enterococcus faecalis infection in Southwest China |
| title_short | Molecular characteristics and risk factors associated with linezolid-resistant Enterococcus faecalis infection in Southwest China |
| title_sort | molecular characteristics and risk factors associated with linezolid resistant enterococcus faecalis infection in southwest china |
| topic | Linezolid resistance Enterococcus faecalis optrA Risk factor |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520300916 |
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