Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta.
The significance of maternal cholesterol transporting to the fetus under normal as well as pathological circumstances is less understood. The objective of this study was to observe the effects of maternal hypercholesterolemia on placental cholesterol transportation. Human full-time placenta, materna...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5310867?pdf=render |
| _version_ | 1818324099217227776 |
|---|---|
| author | Ran Zhang Shan Dong Wei-Wei Ma Xue-Ping Cai Zhi-Yin Le Rong Xiao Qi Zhou Huan-Ling Yu |
| author_facet | Ran Zhang Shan Dong Wei-Wei Ma Xue-Ping Cai Zhi-Yin Le Rong Xiao Qi Zhou Huan-Ling Yu |
| author_sort | Ran Zhang |
| collection | DOAJ |
| description | The significance of maternal cholesterol transporting to the fetus under normal as well as pathological circumstances is less understood. The objective of this study was to observe the effects of maternal hypercholesterolemia on placental cholesterol transportation. Human full-time placenta, maternal and venous cord blood were sampled at delivery from the pregnant women with serum total cholesterol (TC) concentrations at third trimester higher than 7.25 mM (n = 19) and the pregnant women with normal TC concentrations (n = 19). Serum lipids and expression of genes related to cholesterol transportation were measured by western blot or real-time PCR. The results indicated that serum TC, high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) levels were significantly increased, in pregnancies, but decreased in cord blood in hypercholesterolemic group compared to the matched control group. All the subjects were no-drinking, non-smoker, and gestational disease free. The mRNA expression of lipoprotein receptors, including LDLR and VLDLR were significantly increased, while the protein expression of PCSK9 was significantly increased in hypercholesterolemic placenta. In conclusion, maternal hypercholesterolemia might decrease the transportation of cholesterol from mother to fetus because of the high levels of PCSK9 protein expression. |
| first_indexed | 2024-12-13T11:23:12Z |
| format | Article |
| id | doaj.art-05dd047ae0c24871a01215cb2766771c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T11:23:12Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-05dd047ae0c24871a01215cb2766771c2022-12-21T23:48:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017193410.1371/journal.pone.0171934Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta.Ran ZhangShan DongWei-Wei MaXue-Ping CaiZhi-Yin LeRong XiaoQi ZhouHuan-Ling YuThe significance of maternal cholesterol transporting to the fetus under normal as well as pathological circumstances is less understood. The objective of this study was to observe the effects of maternal hypercholesterolemia on placental cholesterol transportation. Human full-time placenta, maternal and venous cord blood were sampled at delivery from the pregnant women with serum total cholesterol (TC) concentrations at third trimester higher than 7.25 mM (n = 19) and the pregnant women with normal TC concentrations (n = 19). Serum lipids and expression of genes related to cholesterol transportation were measured by western blot or real-time PCR. The results indicated that serum TC, high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) levels were significantly increased, in pregnancies, but decreased in cord blood in hypercholesterolemic group compared to the matched control group. All the subjects were no-drinking, non-smoker, and gestational disease free. The mRNA expression of lipoprotein receptors, including LDLR and VLDLR were significantly increased, while the protein expression of PCSK9 was significantly increased in hypercholesterolemic placenta. In conclusion, maternal hypercholesterolemia might decrease the transportation of cholesterol from mother to fetus because of the high levels of PCSK9 protein expression.http://europepmc.org/articles/PMC5310867?pdf=render |
| spellingShingle | Ran Zhang Shan Dong Wei-Wei Ma Xue-Ping Cai Zhi-Yin Le Rong Xiao Qi Zhou Huan-Ling Yu Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta. PLoS ONE |
| title | Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta. |
| title_full | Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta. |
| title_fullStr | Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta. |
| title_full_unstemmed | Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta. |
| title_short | Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta. |
| title_sort | modulation of cholesterol transport by maternal hypercholesterolemia in human full term placenta |
| url | http://europepmc.org/articles/PMC5310867?pdf=render |
| work_keys_str_mv | AT ranzhang modulationofcholesteroltransportbymaternalhypercholesterolemiainhumanfulltermplacenta AT shandong modulationofcholesteroltransportbymaternalhypercholesterolemiainhumanfulltermplacenta AT weiweima modulationofcholesteroltransportbymaternalhypercholesterolemiainhumanfulltermplacenta AT xuepingcai modulationofcholesteroltransportbymaternalhypercholesterolemiainhumanfulltermplacenta AT zhiyinle modulationofcholesteroltransportbymaternalhypercholesterolemiainhumanfulltermplacenta AT rongxiao modulationofcholesteroltransportbymaternalhypercholesterolemiainhumanfulltermplacenta AT qizhou modulationofcholesteroltransportbymaternalhypercholesterolemiainhumanfulltermplacenta AT huanlingyu modulationofcholesteroltransportbymaternalhypercholesterolemiainhumanfulltermplacenta |